2001
DOI: 10.1002/yea.790
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Cooperative functions of the mannoprotein‐encoding genes in the biogenesis and maintenance of the cell wall in Saccharomyces cerevisiae

Abstract: To elucidate the roles of genes involved in the cell wall biogenesis and function in Saccharomyces cerevisiae, we isolated and characterized mutants that were lethal in a strain in which the SED1 gene encoding a cell wall mannoprotein was disrupted. Thus, double mutants of SED1 and either MNN9 or MNN10 were unable to grow and YOL155c on a multicopy plasmid could suppress their synthetic lethality. A Yol155cp-GFP fusion protein was found to localize to the cell wall, suggesting that it might also be a cell wall… Show more

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Cited by 11 publications
(9 citation statements)
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“…Two other genes related with stress response were considered: HSP26 (coding for a temperature-regulated chaperone (Amoró s and Estruch 2001)) and SPI1 (which encodes an important protein in the structure and biogenesis of the cell wall (Horie and Isono 2001)). In both cases the pattern was similar in MS300 and MS60 (data not shown), with increases mainly related with the entry into the stationary phase as described in previous experiments carried out with this strain (Zuzuarregui and del Olmo 2004b) and others (Backhus et al 2001;Carrasco et al 2003;Rossignol et al 2005;Varela et al 2005).…”
Section: Evolution Of the Arginase Activity During Vinificationmentioning
confidence: 99%
“…Two other genes related with stress response were considered: HSP26 (coding for a temperature-regulated chaperone (Amoró s and Estruch 2001)) and SPI1 (which encodes an important protein in the structure and biogenesis of the cell wall (Horie and Isono 2001)). In both cases the pattern was similar in MS300 and MS60 (data not shown), with increases mainly related with the entry into the stationary phase as described in previous experiments carried out with this strain (Zuzuarregui and del Olmo 2004b) and others (Backhus et al 2001;Carrasco et al 2003;Rossignol et al 2005;Varela et al 2005).…”
Section: Evolution Of the Arginase Activity During Vinificationmentioning
confidence: 99%
“…These mutants are expected to have mutations in genes that code for proteins associated with the function of Spf1p. Screening for synthetic lethality with an spf1 disruption mutant was performed by exploiting the ade2/ade3 colony sectoring assay, using strain ANY101 as the reporter (Bender and Pringle 1991;Horie and Isono 2001). After EMS mutagenesis of ANY101 cells (survival rate 44%), about 40,000 colonies were screened.…”
Section: Resultsmentioning
confidence: 99%
“…Screening for synthetic lethality with the spf1 mutation was performed using ade2/ade3 colony sectoring assay (Bender and Pringle 1991;Horie and Isono 2001). ANY101 cells were mutagenized with 3% ethylmethanesulfonate (EMS) for 30 min at 30°C.…”
Section: Screen For Synthetic Lethal Mutantsmentioning
confidence: 99%
“…The use of strains harbouring the mnn9 allele plus the disruption of a specific GPI-CWP-encoding gene has been used recently to highlight co-operative functions in the biogenesis and maintenance of the cell wall in S. cerevisiae (Horie & Isono, 2001). Changes in the cell wall of the disruptant ylcwp1D and of the double disruptant ylcwp1D ylmnn9D were detected by testing their sensitivities to Calcofluor white and Congo red, substances that disturb the cell wall, aggravating the consequences of cell-wall defects (Elorza et al, 1983;Kopecka & Gabriel, 1992;Ram et al, 1994).…”
Section: Discussionmentioning
confidence: 99%