Beef cattle farming assumes a pivotal role in economic growth, household food security, and poverty alleviation in Eswatini. However, paucity of information dissemination, and competence are drawbacks that accord a steady annual increase in beef imports and a decline in exports. Therefore, the study conducted a gender-based comparative assessment of training needs for beef cattle farmers. Primary data were collected through personal interviews, guided by a reliability-tested questionnaire, from a sample of 397 farmers. The Borich Needs Assessment Model was adopted for data analysis and inferential statistics were employed to evaluate statistically significant differences between the gender groups. On a scale of 5, farmers were found to be less proficient (M = 1.891, SD = 0.529) in cattle production and agribusiness management practices. Female farmers were significantly less proficient than males (t = −6.004, p = 0.000). Statistically significant differences in mean weighted discrepancy scores (t = 5.280, p = 0.000) revealed a strong training need for females compared to men. It is recommended that dissemination of training information should be prioritized as follows: (1) agribusiness management concepts, (2) feed and feeding concepts, (3) cattle health concepts, (4) farmer-organizational concepts, (5) farm structures, and (6) breeding and rearing concepts.