Cooperativity in the two-domain arginine kinase from the sea anemone Anthopleura japonicus. II. Evidence from site-directed mutagenesis studies

Gene profiling and characterization of arginine kinase-1 (MrAK-1) from freshwater giant prawn (Macrobrachium rosenbergii)

“…The molecular mass of protein was about 40 kDa on 12% SDS-PAGE gel (Fig. 4), similar to the earlier reported AK of Abe et al [6], Holt and Kinsey [9], Yao et al [15], Wang et al [16], Yao et al [17], Tada and Suzuki [18], Kinsey and Lee [20], Astrofsky et al [23] and Wang et al [24].…”

“…Recently, a number of cDNA from cucumber Stichopus japonicus [1,3], oyster Crassostrea sp [5], kuruma prawn Marsupenaeus japonicus [6], Callinectes sapidus [11], deep-sea clam Calyptogena kaikoi [9] clams Solen strictus and Corbicula japonica [10] chiton Liolophura japonica and turbanshell Battilus cornutus [12], Cellana grata, Aplysia kurodai [13], horseshoe crab Limulus polyphemus [14], sea Chinese shrimp Fenneropenaeus chinensis [15], greasyback shrimp Metapenaeus ensis [16], Litopenaeus vannamei [17] and sea anemone Anthopleura japonicus [18] encoding AKs in major groups of invertebrate have been made available to investigate AK's structure, catalysis mechanism and evolutionary relationship.…”

Arginine kinase in the cladoceran Daphnia magna: cDNA sequencing and expression is associated with resistance to toxic Microcystis

“…The conserved amino acid residues in all AKs, including the 11 amino acid residues (S63, G64, G66, Y68, F194, E225, C271, T273, N274, E314, H315) that interacted with arginine and 14 arg residues (S122, R124, R126, W221, R229, R280, S282, V283, H284, R309, T311, G313, E314, D324) that interacted with ADP, were all identified in CfAK (Tada and Suzuki, 2010). D62 and R197 residues are suggested to play a key role in stabilizing the substrate-bound structures of AK by forming an ion pair (Suzuki et al, 2000) and are conserved in most AK sequences, including the unusual Stichopus AK, which evolved secondarily from CK (Suzuki et al, 1999).…”

Cold-adapted Features of Arginine Kinase from the Deep-sea Clam Calyptogena kaikoi

“…For the Anthopleura japonicas two-domain AK, pET30 plasmid with insert of open reading frame was used (Tada and Suzuki 2010). It was expressed as His-tagged fusion protein in E. coli BL21 cells by induction with 1 mM IPTG at 18°C and at shaking speed of 100 rpm for 93 h, using BioShaker BR-21FP (TAITEC, Koshigaya, Japan).…”