Coordinated photomorphogenic UV-B signaling network captured by mathematical modeling

Abstract

Long-wavelength and low-fluence UV-B light is an informational signal known to induce photomorphogenic development in plants. Using the model plant Arabidopsis thaliana, a variety of factors involved in UV-B-specific signaling have been experimentally characterized over the past decade, including the UV-B light receptor UV resistance locus 8; the positive regulators constitutive photomorphogenesis 1 and elongated hypocotyl 5; and the negative regulators cullin4, repressor of UV-B photomorphogenesis 1 (RUP1), and RUP2. Individual genetic and molecular studies have revealed that these proteins function in either positive or negative regulatory capacities for the sufficient and balanced transduction of photomorphogenic UV-B signal. Less is known, however, regarding how these signaling events are systematically linked. In our study, we use a systems biology approach to investigate the dynamic behaviors and correlations of multiple signaling components involved in Arabidopsis UV-B-induced photomorphogenesis. We define a mathematical representation of photomorphogenic UV-B signaling at a temporal scale. Supplemented with experimental validation, our computational modeling demonstrates the functional interaction that occurs among different protein complexes in early and prolonged response to photomorphogenic UV-B.light signaling | ordinary differential equation

Two E3 ligases antagonistically regulate the UV-B response in Arabidopsis

“…Antibodies Used for Immunoblotting. The following primary antibodies used in this study are commercially available or were described previously: anti-GST (Sino Biological, Inc.), anti-c-Myc (Thermo Fisher Scientific), anti-FLAG (Sigma-Aldrich), anti-MBP (TransGen Biotech), anti-GFP (Thermo Fisher Scientific), anti-Ubiquitin (Cell Signaling Technology), anti-DDB1 (38), anti-RUP2 (17), anti-UVR8 (12), and anti-HA (Sigma-Aldrich). The anti-RUP1, anti-RPN6, and anti-HY5 antibodies were generated in this study by raising rabbit polyclonal antibodies against His-RUP1, His-RPN6, and His-HY5 recombinant proteins, respectively.…”

“…In addition, the nuclear UVR8 interacts with the transcription factors WRKY DNA-BINDING PROTEIN 36 (WRKY36), BRI1-EMS-SUPPRESSOR 1 (BES1), and BES1-INTERACTING MYC-LIK1 (BIM1) to regulate UV-B responsive gene expression (13,14). On the other hand, two homologous proteins, REPRESSOR OF UV-B PHOTOMORPHOGENESIS 1 (RUP1) and RUP2, function redundantly to negatively regulate UV-B-induced photomorphogenesis by facilitating UVR8 redimerization and disturbing the UVR8-COP1 interaction (15)(16)(17)(18).…”

Two Distinct Domains of the UVR8 Photoreceptor Interact with COP1 to Initiate UV-B Signaling in Arabidopsis

“…UVR8-COP1 complex formation results in the induction of RUP1 and RUP2 expression, forming a negative feedback loop that facilitates UVR8 redimerization and thus disruption of the UVR8-COP1 interaction Ouyang et al, 2014). It was shown previously that RUP1 and RUP2 interact with the C27 domain of UVR8, potentially competing with COP1 (Cloix et al, 2012).…”

Studies on plant responses to environmental change in China: the past and the future

“…术培育抗铝农作物新品种, 从而高效利用酸性土壤资 源奠定了理论基础. [22] , 鉴定了 UVR8信号通路的新因子 [139,140] , 揭示了其核心蛋白复 合体的组装与功能 [141,142] 、UVR8核定位调控基因表 达的分子机制 [143~145] , 并构建了紫外光初级信号网络 的数学模型 [146] ; 发现了CRY调控气孔开放并与phyA 和phyB共同介导光信号促进气孔发育的功能 [147,148] , phyB在远红光下调控光形态建成的新功能 [149] 以及 phyB信号同时调控种子休眠与萌发的双重功能 [150] ;…”