Coordination and fine motor control depend on Drosophila TRPγ

Akitake, Bradley; Ren, Qiuting; Boiko, Nina; Ni, Jinfei D.; Sokabe, Takaaki; Stockand, James D.; Eaton, Benjamin A.; Montell, Craig

doi:10.1038/ncomms8288

Cited by 45 publications

(44 citation statements)

References 57 publications

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“…These include gustatory receptors (GRs), ionotropic receptors (IRs) and transient receptor potential (TRP) channels, several of which are known mechanosensors involved in touch, proprioception and hearing (Akitake et al, 2015; Benton et al, 2009; Effertz et al, 2012; Koh et al, 2014; Walker et al, 2000; Yan et al, 2013). None of these candidates were required for sensing food mechanics (Figure 2A).…”

Section: Resultsmentioning

confidence: 99%

The Basis of Food Texture Sensation in Drosophila

Zhang

Aikin

et al. 2016

Neuron

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104

124

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Summary Food texture has enormous affects on food preferences. However, the mechanosensory cells and key molecules responsible for sensing the physical properties of food are unknown. Here, we show that akin to mammals, the fruit fly, Drosophila melanogaster, prefers food with a specific hardness or viscosity. This food texture discrimination depends upon a previously unknown multidendritic (md-L) neuron, which extends elaborate dendritic arbors innervating the bases of taste hairs. The md-L neurons exhibit directional selectivity in response to mechanical stimuli. Moreover, these neurons orchestrate different feeding behaviors depending on the magnitude of the stimulus. We demonstrate that the single Drosophila transmembrane channel-like (TMC) is expressed in md-L neurons, where it is required for sensing two key textural features of food—hardness and viscosity. We propose that md-L neurons are long-sought-after mechanoreceptor cells through which food mechanics are perceived and encoded by a taste organ, and this sensation depends on TMC.

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Section: Resultsmentioning

confidence: 99%

The Basis of Food Texture Sensation in Drosophila

Zhang

Aikin

et al. 2016

Neuron

Self Cite

104

124

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“…As mentioned above, the TRPC channel TRPγ is required in proprioceptive chordotonal neurons but not in Johnston's organ. 21) While Iav and Nan occur together in chordotonal neurons as subunits of a heteromeric TRPV channel that is activated by pyridine azomethine insecticides, the individual distribution of these TRPs is broader. Iav is expressed without Nan in motor neurons, where it acts as a key regulator of synaptic develop- ment and function by influencing presynaptic resting Ca 2+ concentration.…”

Section: Trpvmentioning

confidence: 99%

Insect TRP channels as targets for insecticides and repellents

Salgado

2017

Journal of Pesticide Science

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This review provides a brief overview of ion channels, then focuses on TRP channels, describing the properties and functions of the seven TRP channel classes found in insects. Finally, recent work showing that a heteromeric channel composed of Nanchung and Inactive vanilloid TRP (TRPV) channel subunits is the target of the selective feeding blockers pymetrozine and pyrifluquinazon is described. The possible utility of other TRP channels as targets of insecticides and repellents is also considered. © Pesticide Science Society of Japan

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“…Trp-γ 1 mutants show only fine motor control defects (3). Interestingly, these flies manifested numerous descending tracks along with the ascending tracks and this was not because of less overall movement of these flies.…”

Section: Discussionmentioning

confidence: 92%

A cost effective high-resolution climbing assay applied to Drosophila Parkinson’s and proprioception mutants reveal novel behavioural phenotypes

Aggarwal

Reichert

VijayRaghavan

2018

Preprint

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Severe locomotor impairment is a common phenotype of neurodegenerative disorders such as Parkinson's disease (PD). Drosophila models of PD, studied from more than a decade, have helped in understanding the interaction between various genetic factors, such as parkin and PINK1, in this disease. To characterize locomotor behavioural phenotypes for these genes, fly climbing assays have been widely used. While these simple current assays for locomotor defects in Drosophila mutants measure some locomotor phenotypes well, it is possible that detection of subtle changes in behaviour is important to understand the early manifestation of locomotor disorders. We introduce a novel climbing behaviour assay which provides such fine-scale behavioural data and tests this proposition for the Drosophila model. We use this inexpensive, fully automated, high resolution assay to quantitatively characterize the parameters of climbing behaviour in three contexts. First, we characterize wild type flies and uncover a hitherto unknown sexual dimorphism in climbing behaviour. Second, we study climbing behaviour of heterozygous mutants of genes implicated in the fly PD model and reveal previously unreported prominent locomotor defects in some of these heterozygous fly lines. Finally, we study locomotor defects in a homozygous proprioceptory mutation (Trp-γ 1 ) known to affect fine motor control in Drosophila.Moreover, we identify aberrant geotactic behaviour in Trp-γ 1 mutants, thereby opening up a finer assay for geotaxis and its genetic basis. Our assay is therefore a cost-effective, general tool for measuring locomotor behaviours of wild type and mutant flies in fine detail and can reveal mild motor defects.Fine control of neuronal activity is required for proper motor output. Severe locomotor impairment is a common result of neurodegenerative disorders such as Parkinson's disease (PD). The fruitfly, Drosophila, has been widely used as a model system to study the genetics of these disorders and simple climbing assays have been used to study the behavioural phenotypes of mutations in these genes. Here we introduce a novel, fully automated, high resolution climbing behaviour assay and use this assay to characterize climbing behaviour in wild type flies and in various fly mutant lines related to PD and defects in proprioception. Our assay is a general tool for measuring locomotor behaviours of flies in fine detail and can reveal very mild motor defects.

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Coordination and fine motor control depend on Drosophila TRPγ

Cited by 45 publications

References 57 publications

The Basis of Food Texture Sensation in Drosophila

The Basis of Food Texture Sensation in Drosophila

Insect TRP channels as targets for insecticides and repellents

A cost effective high-resolution climbing assay applied to Drosophila Parkinson’s and proprioception mutants reveal novel behavioural phenotypes

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