Coordination of erythropoiesis by the transcription factor c-Myb

Vegiopoulos, Alexandros; García, Paloma; Emambokus, Nikla; Frampton, Jon

doi:10.1182/blood-2005-07-2968

Cited by 102 publications

(98 citation statements)

References 42 publications

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“…Ter119 and CD71 double positive erythroblasts were collected using a Beckman Coulter MoFlo high speed cell sorter. For preparation of proerythroblasts from fetal liver, single cell suspensions of fetal liver cells from E12.5 mouse embryos were cultured as described (37). In brief, prepared cells were cultured in Stempro 34 complete medium supplemented with 100 ng/ml murine stem cell factor (PeproTech), 2 units/ml human erythropoietin (Roche Applied Science), and 1 M dexamethasone (Sigma) for 7 days.…”

Section: Methodsmentioning

confidence: 99%

The TspanC8 Subgroup of Tetraspanins Interacts with A Disintegrin and Metalloprotease 10 (ADAM10) and Regulates Its Maturation and Cell Surface Expression

Haining

Yang

Bailey

et al. 2012

Journal of Biological Chemistry

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142

201

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Background: ADAM10 is a transmembrane metalloprotease that regulates development, inflammation, cancer, and Alzheimer disease. Results: The TspanC8 subgroup of tetraspanin membrane proteins interacts with and promotes ADAM10 maturation and cell surface localization. Conclusion: This study defines the TspanC8 tetraspanins as essential regulators of ADAM10. Significance: Focusing on specific TspanC8-ADAM10 complexes may allow ADAM10 therapeutic targeting in a cell typeand/or substrate-specific manner.

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Section: Methodsmentioning

confidence: 99%

The TspanC8 Subgroup of Tetraspanins Interacts with A Disintegrin and Metalloprotease 10 (ADAM10) and Regulates Its Maturation and Cell Surface Expression

Haining

Yang

Bailey

et al. 2012

Journal of Biological Chemistry

Self Cite

142

201

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“…miR-150 (decreased in PV cells) is predicted to target cMYB proto-oncogene. This gene plays an important role in erythropoiesis by maintaining proliferation at early stages and it may inhibit terminal erythroid differentiation [31]. Expression of cMYB is also associated with c-KIT expression in erythroid cells [31].…”

Section: In Silico Search For Target Genes Of Studied Mirnasmentioning

confidence: 99%

Regulated expression of microRNAs in normal and polycythemia vera erythropoiesis

Bruchova

Yoon

Agarwal

et al. 2007

Experimental Hematology

189

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Objective-Polycythemia vera (PV) is a myeloproliferative disorder, arising from the acquired mutation(s) of a hematopoietic stem cell. The JAK2 V617F somatic mutation is found in most PV patients; however, it is not the disease-initiating mutation. Since microRNAs (miRNAs) play a regulatory role in hematopoiesis, we studied miRNA expressions in PV and normal erythropoiesis.Methods-The peripheral blood mononuclear cells were cultured in a three-phase liquid system resulting in synchronized expansion of erythroid progenitors. Using gene expression profiling by CombiMatrix MicroRNArray, we searched for PV specific changes at day1, 14 and 21. Twelve miRNA candidates were then reevaluated by quantitative RealTime PCR in a larger number of samples obtained from progenitors at the same stage of differentiation.Results-A significant difference of miR-150 expression was found in PV. In normal erythropoiesis, three expression patterns of miRNAs were observed: progressive down-regulation of miR-150, miR-155, miR-221, miR-222; up-regulation of miR-451, miR-16 at late stages of erythropoiesis; and biphasic regulation of miR-339, miR-378. The miR-451 appears to be erythroid specific.Conclusions-We identified the miRNAs with regulated expression in erythropoiesis; one appeared to be PV specific. Their miRNA expression levels define early, intermediate, and late stages of erythroid differentiation. The validity of our findings was confirmed in non-expanded peripheral blood cells.

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“…Mice with a non-functional c-myb gene suffer from severe anaemia and die by embryonic day 15 (Mucenski et al, 1991). More recent studies have uncovered that distinct threshold levels of c-Myb are required at different stages of hematopoiesis (Emambokus et al, 2003;Sakamoto et al, 2006;Vegiopoulos et al, 2006). Two independent genetic screenings in mice have identified three point mutations in c-myb affecting specific stages of hematopoiesis causing a range of hematopoietic defects (Carpinelli et al, 2004;Sandberg et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

FLASH acts as a co-activator of the transcription factor c-Myb and localizes to active RNA polymerase II foci

et al. 2008

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The c-Myb oncoprotein is a DNA-binding transcription factor with a key role in early stages of hematopoiesis. To expand our knowledge of partners cooperating with c-Myb, we performed a yeast two-hybrid screening with full-length c-Myb as bait. Here, we report FLICEassociated huge protein (FLASH)/CASP8AP2 as a novel Myb-interacting protein. We show that FLASH interacts with the DNA-binding domain of c-Myb and enhances c-Myb-dependent reporter activity and expression of endogenous c-Myb target genes. Chromatin immunoprecipitation assays revealed that FLASH and c-Myb both associate with the MYC promoter region as well as with the intronic enhancer of the c-Myb target gene ADA. Furthermore, siRNA knock-down of FLASH or c-Myb both result in a reduction of MYC and ADA expression. The co-activator effect is mediated through the C-terminal part of FLASH, which binds c-Myb. The FLASH-induced enhancement is comparable with the increase seen with the c-Myb co-activator p300. We find FLASH localized in discrete nuclear speckles in several cell lines, co-localized with c-Myb in active RNA polymerase II foci. These results imply a novel molecular mechanism of regulation of c-Myb activity. We propose that c-Myb cooperates with FLASH in foci associated with active RNA polymerase II, leading to enhancement of Myb-dependent gene activation.

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Coordination of erythropoiesis by the transcription factor c-Myb

Cited by 102 publications

References 42 publications

The TspanC8 Subgroup of Tetraspanins Interacts with A Disintegrin and Metalloprotease 10 (ADAM10) and Regulates Its Maturation and Cell Surface Expression

The TspanC8 Subgroup of Tetraspanins Interacts with A Disintegrin and Metalloprotease 10 (ADAM10) and Regulates Its Maturation and Cell Surface Expression

Regulated expression of microRNAs in normal and polycythemia vera erythropoiesis

FLASH acts as a co-activator of the transcription factor c-Myb and localizes to active RNA polymerase II foci

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