Coordination of insulin and Notch pathway activities by microRNA miR-305 mediates adaptive homeostasis in the intestinal stem cells of the <i>Drosophila</i> gut

Foronda, David; Weng, Ruifen; Verma, Pushpa; Chen, Yawen; Cohen, Stephen M.

doi:10.1101/gad.241588.114

Cited by 71 publications

(70 citation statements)

References 35 publications

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“…This probably leads to elevated levels of InR protein in nutrient-deprived cells, which sensitizes them to insulin and thereby ensures a rapid response when growth conditions are restored. Lin-28 might also regulate InR via a 3′UTR mechanism, as it was recently shown that the microRNA miR-305 negatively regulates InR levels in ISCs via its 3′UTR (Foronda et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Lin-28 promotes symmetric stem cell division and drives adaptive growth in the adult Drosophila intestine

2015

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Stem cells switch between asymmetric and symmetric division to expand in number as tissues grow during development and in response to environmental changes. The stem cell intrinsic proteins controlling this switch are largely unknown, but one candidate is the Lin-28 pluripotency factor. A conserved RNA-binding protein that is downregulated in most animals as they develop from embryos to adults, Lin-28 persists in populations of adult stem cells. Its function in these cells has not been previously characterized. Here, we report that Lin-28 is highly enriched in adult intestinal stem cells in the Drosophila intestine. lin-28 null mutants are homozygous viable but display defects in this population of cells, which fail to undergo a characteristic food-triggered expansion in number and have reduced rates of symmetric division as well as reduced insulin signaling. Immunoprecipitation of Lin-28-bound mRNAs identified Insulin-like Receptor (InR), forced expression of which completely rescues lin-28-associated defects in intestinal stem cell number and division pattern. Furthermore, this stem cell activity of lin-28 is independent of one well-known lin-28 target, the microRNA let-7, which has limited expression in the intestinal epithelium. These results identify Lin-28 as a stem cell intrinsic factor that boosts insulin signaling in intestinal progenitor cells and promotes their symmetric division in response to nutrients, defining a mechanism through which Lin-28 controls the adult stem cell division patterns that underlie tissue homeostasis and regeneration.

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Section: Discussionmentioning

confidence: 99%

Lin-28 promotes symmetric stem cell division and drives adaptive growth in the adult Drosophila intestine

2015

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“…Furthermore, 20E is a major regulator of reproductive events PBM; however, its role in regulating digestive enzymes is not understood. Several insect miRNAs have been shown to be regulated by 20E [20][21][22][23][24] and insulin [25][26][27] signaling pathways. Because mature miR-1890 levels are enhanced PBM, we investigated the role of insulin and 20E signaling pathways in regulating miR-1890 expression.…”

Section: Discussionmentioning

confidence: 99%

Mosquito-specific microRNA-1890 targets the juvenile hormone-regulated serine protease JHA15 in the female mosquito gut

et al. 2015

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S Raikhel (2015) Mosquito-specific microRNA-1890 targets the juvenile hormone-regulated serine protease JHA15 in the female mosquito gut, RNA Biology, 12:12, 1383-1390, DOI: 10.1080/15476286.2015 Females of the hematophagous mosquito species require a vertebrate blood meal to supply amino acids and other nutrients necessary for egg development, serving as the driving force for the spread of many vector-borne diseases in humans. Blood digestion utilizes both early and late phase serine proteases (SPs) that are differentially regulated at the transcriptional and post-transcriptional level. To uncover the regulatory complexity of SPs in the female mosquito midgut, we investigated involvement of miRNAs in regulating the juvenile hormone (JH)-controlled chymotrypsin-like SP, JHA15. We identified regulatory regions complementary to the mosquito-specific miRNA, miR-1890, within the 3 0 UTR of JHA15 mRNA. The level of the JHA15 transcript is highest post eclosion and drastically declines post blood meal (PBM), exhibiting an opposite trend to miR-1890 that peaks at 24 h PBM. Depletion of miR-1890 results in defects in blood digestion, ovary development and egg deposition. JHA15 mRNA and protein levels are elevated in female mosquitoes with miR-1890 inhibition. JHA15 RNA interference in the miR-1890 depletion background alleviates miR-1890 depletion phenotypes. The miR-1890 gene is activated by the 20-hydroxyecdysone pathway that involves the ecdysone receptor and the early genes, E74B and Broad Z2. Our study suggests that miR-1890 controls JHA15 mRNA stability in a stage-and tissue-specific manner.

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“…The mir-317 influences reproductive responses by females to male sex peptide (Fricke et al 2014) and was involved in cell cycle control in association with G2/M cyclin B in Drosophila (Pushpavalli et al 2014). miR-305 regulates the Notch and insulin pathways in the intestinal stem cells, which are required for adaptive homeostasis in the Drosophila gut (Foronda et al 2014). mir-2788a plays a potential role in butterfly wing development in Heliconius (Surridge et al 2011).…”

Section: Discussionmentioning

confidence: 99%

The identification and characteristics of salinity-related microRNAs in gills of Portunus trituberculatus

Liu

Gao

et al. 2016

Cell Stress and Chaperones

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MicroRNAs (miRNAs) are small noncoding RNAs that post-transcriptionally regulate gene expression in organisms. To understand the underlying mechanisms behind the molecular response of the crab to low salt-stress, highthroughput Illumina/Solexa deep sequencing technology was used to investigate the expression profiles of miRNAs under low salinity challenged. Two mixed RNA pool libraries of gill tissues from low salinity challenged (LC) and the control groups (NC) were sequenced on the Illumina platform. A total of 6,166,057 and 7,032,973 high-quality reads were obtained from the NC and LC libraries, respectively. Sixty-seven miRNAs consisting of 16 known and 51 novel ones were identified, among which, 12 miRNAs were differentially expressed in LC compared to NC. Thirty-four of the target genes predicted were differentially expressed in the opposite direction to the miRNAs, which were involved in crucial processes related to osmoregulation by gene ontology (GO) functional enrichment analysis, such as anion transport processes (GO:0006820) and chitin metabolic process (GO:0006030). These results provide a basis for further investigation of the miRNA-modulating networks in osmoregulation of Portunus trituberculatus.

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Coordination of insulin and Notch pathway activities by microRNA miR-305 mediates adaptive homeostasis in the intestinal stem cells of the Drosophila gut

Cited by 71 publications

References 35 publications

Lin-28 promotes symmetric stem cell division and drives adaptive growth in the adult Drosophila intestine

Lin-28 promotes symmetric stem cell division and drives adaptive growth in the adult Drosophila intestine

Mosquito-specific microRNA-1890 targets the juvenile hormone-regulated serine protease JHA15 in the female mosquito gut

The identification and characteristics of salinity-related microRNAs in gills of Portunus trituberculatus

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