Copine-I represses NF-κB transcription by endoproteolysis of p65

Ramsey, Catherine S.; Yeung, Fan; Stoddard, Paul B; Li, D; Creutz, Carl E.; Mayo, Marty W.

doi:10.1038/sj.onc.1211030

Cited by 45 publications

(44 citation statements)

References 42 publications

(53 reference statements)

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“…The vWA domain mediates proteineprotein interaction [8]. Moreover, it has been demonstrated that copines translocate from cytoplasm into the nucleus (CPNE1, 2, 3, and 7) [9], regulate NF-kB gene expression (CPNE1) [10], and interact with the protein nucleolin (CPNE3) [11]. However, there have been few studies about whether Cpne7 can regulate odontoblastic differentiation of mesenchymal cells.…”

Section: Introductionmentioning

confidence: 98%

CPNE7, a preameloblast-derived factor, regulates odontoblastic differentiation of mesenchymal stem cells

Choung

Lee

et al. 2015

Biomaterials

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Section: Introductionmentioning

confidence: 98%

CPNE7, a preameloblast-derived factor, regulates odontoblastic differentiation of mesenchymal stem cells

Choung

Lee

et al. 2015

Biomaterials

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“…Plasmid and siRNA transfections and luciferase assays were performed as described (25). QRT-PCR experiments were carried out as described (48). PCR primers are shown in Table S1.…”

Section: Methodsmentioning

confidence: 99%

Modification of RelA by O - linked N -acetylglucosamine links glucose metabolism to NF-κB acetylation and transcription

Allison¹,

Wamsley²,

Kumar³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

100

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The molecular mechanisms linking glucose metabolism with active transcription remain undercharacterized in mammalian cells. Using nuclear factor-κB (NF-κB) as a glucose-responsive transcription factor, we show that cells use the hexosamine biosynthesis pathway and O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) to potentiate gene expression in response to tumor necrosis factor (TNF) or etoposide. Chromatin immunoprecipitation assays demonstrate that, upon induction, OGT localizes to NF-κB-regulated promoters to enhance RelA acetylation. Knockdown of OGT abolishes p300-mediated acetylation of RelA on K310, a posttranslational mark required for full NF-κB transcription. Mapping studies reveal T305 as an important residue required for attachment of the O-GlcNAc moiety on RelA. Furthermore, p300 fails to acetylate a full-length RelA(T305A) mutant, linking O-GlcNAc and acetylation events on NF-κB. Reconstitution of RelA null cells with the RelA(T305A) mutant illustrates the importance of this residue for NF-κB-dependent gene expression and cell survival. Our work provides evidence for a unique regulation where attachment of the O-GlcNAc moiety to RelA potentiates p300 acetylation and NF-κB transcription.p65 | NF kappa B | apoptosis

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“…Ramsey et al [11] showed that copine I inhibits NF-κB regulated transcription in response to TNFα in prostate cancer cell lines by inducing the proteolysis of the N-terminus of p65, one of the subunits of NF-κB. These authors suggest the copine I may associate through its A domain with protease-containing protein complexes to regulate proteolysis of NF-κB subunits.…”

Section: Introductionmentioning

confidence: 99%

Copine A plays a role in the differentiation of stalk cells and the initiation of culmination in Dictyostelium development

et al. 2010

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BackgroundCopines are calcium-dependent phospholipid-binding proteins found in diverse eukaryotic organisms. We are studying the function of copines in Dictyostelium discoideum, a single-celled amoeba that undergoes cell differentiation and morphogenesis to form multicellular fruiting bodies when placed in starvation conditions. Previously, we showed that Dictyostelium cells lacking the copine A (cpnA) gene are not able to complete the developmental cycle, arresting at the slug stage. The aim of this study is to further characterize the developmental defect of the cpnA- cells.ResultsTime-lapse imaging revealed that cpnA- cells exhibited delayed aggregation and made large mounds that formed one large slug as compared to the smaller slugs of the wild-type cells. While the prespore cell patterning appeared to be normal within the cpnA- slugs, the prestalk cell patterning was different from wild-type. When cpnA- cells were mixed with a small percentage of wild-type cells, chimeric fruiting bodies with short stalks formed. When a small percentage of cpnA- cells was mixed with wild-type cells, the cpnA- cells labeled with GFP were found located throughout the chimeric slug and in both the stalk and sporehead of the fruiting bodies. However, there appeared to be a small bias towards cpnA- cells becoming spore cells. When cpnA- cells were developed in buffer containing EGTA, they were also able to differentiate into either stalk or spore cells to form fruiting bodies with short stalks.ConclusionsOur results indicate that CpnA is involved in the regulation of aggregation, slug size, and culmination during Dictyostelium development. More specifically, CpnA appears to be involved in the function and differentiation of prestalk cells and plays a role in a calcium-regulated signaling pathway critical to triggering the initiation of culmination.

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Copine-I represses NF-κB transcription by endoproteolysis of p65

Cited by 45 publications

References 42 publications

CPNE7, a preameloblast-derived factor, regulates odontoblastic differentiation of mesenchymal stem cells

CPNE7, a preameloblast-derived factor, regulates odontoblastic differentiation of mesenchymal stem cells

Modification of RelA by O - linked N -acetylglucosamine links glucose metabolism to NF-κB acetylation and transcription

Copine A plays a role in the differentiation of stalk cells and the initiation of culmination in Dictyostelium development

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