Copolymers and enzymes blended as LB films changing the bioelectronics properties of interfaces

Barbosa, Camila Gouveia; La-Scalea, Mauro Aquiles; Caseli, Luciano; Péres, Laura O.

doi:10.1016/j.colcom.2018.10.001

Cited by 6 publications

(4 citation statements)

References 37 publications

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“…Biosensors containing urease, algal polysaccharides, CNT, and DODAB were used to detect urea with capacitance–voltage and constant capacitance measurements . Semiconducting polymers can also be used in flexible matrices providing stable enzyme activity in LB films, e.g., for phytase to detect phytic acid and urease to detect urea. , The cartoon in Figure illustrates the procedure to fabricate urease-containing LB films for detecting urea where the organized nature of the film facilitated analyte diffusion and electron transfer.…”

Section: Lb Films Used In Molecular (Organic) Electronics and Sensorsmentioning

confidence: 99%

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The Past and the Future of Langmuir and Langmuir–Blodgett Films

2022

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The Langmuir–Blodgett (LB) technique, through which monolayers are transferred from the air/water interface onto a solid substrate, was the first method to allow for the controlled assembly of organic molecules. With its almost 100 year history, it has been the inspiration for most methods to functionalize surfaces and produce nanocoatings, in addition to serving to explore concepts in molecular electronics and nanoarchitectonics. This paper provides an overview of the history of Langmuir monolayers and LB films, including the potential use in devices and a discussion on why LB films are seldom considered for practical applications today. Emphasis is then given to two areas where these films offer unique opportunities, namely, in mimicking cell membrane models and exploiting nanoarchitectonics concepts to produce sensors, investigate molecular recognitions, and assemble molecular machines. The most promising topics for the short- and long-term prospects of the LB technique are also highlighted.

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Section: Lb Films Used In Molecular (Organic) Electronics and Sensorsmentioning

confidence: 99%

Section: Lb Films Used In Molecular (Organic) Electronics and Sensorsmentioning

confidence: 99%

The Past and the Future of Langmuir and Langmuir–Blodgett Films

2022

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“…23−25 The use of this polymer has reported to serve as a matrix to immobilize the enzyme urease, which motivated this work. 26,27 In particular, Langmuir and LB films have been used not only for basic studies 28,29 but also for bionanotechnology, 30−34 which justifies this strategy to build sensors with optimized performance.…”

Section: ■ Introductionmentioning

confidence: 99%

“…These films were then characterized and the viability as biosensors was evaluated by catalytic activity measurements. Particularly, conjugated polymers are interesting because they present several applications, such as sensors, devices, and solar cells. − The use of this polymer has reported to serve as a matrix to immobilize the enzyme urease, which motivated this work. , In particular, Langmuir and LB films have been used not only for basic studies , but also for bionanotechnology, − which justifies this strategy to build sensors with optimized performance.…”

Section: Introductionmentioning

confidence: 99%

Conjugated Polymers Blended with Lipids and Galactosidase as Langmuir–Blodgett Films To Control the Biosensing Properties of Nanostructured Surfaces

2019

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The structure of enzymes must be conserved when incorporated in nanoelectronic devices because their activity determines the function of the device as sensors. Among the systems that can retain their conformational structures, Langmuir–Blodgett (LB) films can be useful to exploit the construction of bioelectronic devices organized at the molecular level because biological and polymeric materials can be coupled as ultrathin films for biosensors and actuators. In this paper, we immobilized a β-galactosidase enzyme in the LB films of stearic acid and the conjugated polymer poly[(9,9-dioctylfluorene)-co-thiophene]. After the characterization of the floating films using tensiometry, vibrational spectroscopy, and Brewster angle microscopy, they were transferred to solid supports as LB films, and the catalytic activity of the enzyme could be preserved as analyzed using UV–vis spectroscopy. We noted that the presence of a supramolecular structure formed in the LB films not only conserved the enzyme activity but also exhibited regular and distinctive output signals in all molecular architectures employed in this work. These results are related to the synergism between the compounds on the active layer associated with a surface morphology that facilitated the analyte diffusion because of an adequate molecular accommodation of all components. This work then demonstrates the viability of employing LB films composed of lipids, enzymes, and synthetic polymers as devices for biosensing applications.

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