Copper amine oxidase 8 regulates arginine-dependent nitric oxide production in Arabidopsis thaliana

Groß, Felicitas; Rudolf, Eva-Esther; Thiele, Björn; Durner, Jörg; Astier, Jérémy

doi:10.1093/jxb/erx105

Cited by 54 publications

(28 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The up-regulation of both genes in the sensitive genotype can be linked to the positive phenotypic effect of the allele from Syn86 in the variation of kernel weight under salt stress [ 27 ]. Studies in Arabidopsis have shown the involvement of copper amine oxidases in the biosynthesis of nitric oxide, which is a signaling molecule that participates in adaptive responses to biotic or abiotic stresses [ 69 – 71 ]. On the other hand, amino acid transporters up-regulated by salt stress can be involved in the transport of amino acids, such as proline, which accumulates under stress to act as an osmolyte for osmotic adjustment [ 72 , 73 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling at osmotic and ionic phases of salt stress response in bread wheat uncovers trait-specific candidate genes

et al. 2020

View full text Add to dashboard Cite

Background Bread wheat is one of the most important crops for the human diet, but the increasing soil salinization is causing yield reductions worldwide. Improving salt stress tolerance in wheat requires the elucidation of the mechanistic basis of plant response to this abiotic stress factor. Although several studies have been performed to analyze wheat adaptation to salt stress, there are still some gaps to fully understand the molecular mechanisms from initial signal perception to the onset of responsive tolerance pathways. The main objective of this study is to exploit the dynamic salt stress transcriptome in underlying QTL regions to uncover candidate genes controlling salt stress tolerance in bread wheat. The massive analysis of 3′-ends sequencing protocol was used to analyze leave samples at osmotic and ionic phases. Afterward, stress-responsive genes overlapping QTL for salt stress-related traits in two mapping populations were identified. Results Among the over-represented salt-responsive gene categories, the early up-regulation of calcium-binding and cell wall synthesis genes found in the tolerant genotype are presumably strategies to cope with the salt-related osmotic stress. On the other hand, the down-regulation of photosynthesis-related and calcium-binding genes, and the increased oxidative stress response in the susceptible genotype are linked with the greater photosynthesis inhibition at the osmotic phase. The specific up-regulation of some ABC transporters and Na+/Ca2+ exchangers in the tolerant genotype at the ionic stage indicates their involvement in mechanisms of sodium exclusion and homeostasis. Moreover, genes related to protein synthesis and breakdown were identified at both stress phases. Based on the linkage disequilibrium blocks, salt-responsive genes within QTL intervals were identified as potential components operating in pathways leading to salt stress tolerance. Furthermore, this study conferred evidence of novel regions with transcription in bread wheat. Conclusion The dynamic transcriptome analysis allowed the comparison of osmotic and ionic phases of the salt stress response and gave insights into key molecular mechanisms involved in the salt stress adaptation of contrasting bread wheat genotypes. The leveraging of the highly contiguous chromosome-level reference genome sequence assembly facilitated the QTL dissection by targeting novel candidate genes for salt tolerance.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling at osmotic and ionic phases of salt stress response in bread wheat uncovers trait-specific candidate genes

et al. 2020

View full text Add to dashboard Cite

show abstract

“…These have been shown to enhance the expression of important Chl biosynthesis genes, including HEMA1, CAO1, YGL1, and PORAl, to increase rRNA processing in chloroplasts, and to promote chloroplast development (Wu et al, 2007;Romani et al, 2012;Tiller et al, 2012;Gong et al, 2013;Liu et al, 2014;Tiller and Bock, 2014). In addition, isoprene-responsive gene expression favored enhanced CK signaling (KFB20), reduction of premature senescing (SAUL1 and TEM1), and polyamine biosynthesis genes in favor of spermidine and spermine synthesis (ADC1, ADC2, and CuAO3), all of which can promote Chl biosynthesis and retention (Capell et al, 2004;Mason et al, 2005;Raab et al, 2009;Woo et al, 2010;Liu et al, 2015;Groß et al, 2017).…”

Section: Isoprene Has a Positive Effect On Chlorophyll And Carotenoidmentioning

confidence: 99%

“…Alterations in putrescine and spermidine content enhance thermotolerance, alleviate heat-induced damage, increase antioxidant enzyme activity, and reduce the accumulation of H 2 O 2 (Sagor et al, 2013;Kumar et al, 2014;Liu et al, 2015). Enhanced spermine and spermidine helps retain Chl and protects plants against salt and drought stress (Capell et al, 2004;Zarei et al, 2016;Groß et al, 2017;Montilla-Bascón et al, 2017). NAD(H) kinase1 remedies oxidative damage under a variety of stresses, including H 2 O 2 and ozone (Berrin et al, 2005;Chai et al, 2006;Hashida et al, 2009).…”

Section: Isoprene Executes Its Protective Functions By Altering Exprementioning

confidence: 99%

Isoprene Acts as a Signaling Molecule in Gene Networks Important for Stress Responses and Plant Growth

et al. 2019

View full text Add to dashboard Cite

Isoprene synthase converts dimethylallyl diphosphate to isoprene and appears to be necessary and sufficient to allow plants to emit isoprene at significant rates. Isoprene can protect plants from abiotic stress but is not produced naturally by all plants; for example, Arabidopsis (Arabidopsis thaliana) and tobacco (Nicotiana tabacum) do not produce isoprene. It is typically present at very low concentrations, suggesting a role as a signaling molecule; however, its exact physiological role and mechanism of action are not fully understood. We transformed Arabidopsis with a Eucalyptus globulus isoprene synthase. The regulatory mechanisms of photosynthesis and isoprene emission were similar to those of native emitters, indicating that regulation of isoprene emission is not specific to isoprene-emitting species. Leaf chlorophyll and carotenoid contents were enhanced by isoprene, which also had a marked positive effect on hypocotyl, cotyledon, leaf, and inflorescence growth in Arabidopsis. By contrast, leaf and stem growth was reduced in tobacco engineered to emit isoprene. Expression of genes belonging to signaling networks or associated with specific growth regulators (e.g. gibberellic acid that promotes growth and jasmonic acid that promotes defense) and genes that lead to stress tolerance was altered by isoprene emission. Isoprene likely executes its effects on growth and stress tolerance through direct regulation of gene expression. Enhancement of jasmonic acid-mediated defense signaling by isoprene may trigger a growth-defense tradeoff leading to variations in the growth response. Our data support a role for isoprene as a signaling molecule.

show abstract

“…Their genes have been identified in several species, as, for example, Arabidopsis (Møller and McPherson, 1998; Planas-Portell et al., 2013), chickpea (Rea et al., 1998), pea (Tipping and McPherson, 1995), tobacco (Paschalidis and Roubelakis-Angelakis, 2005b; Naconsie et al., 2014), apple (Zarei et al., 2015), grapevine (Paschalidis et al., 2009b), and sweet orange (Wang et al., 2017). Arabidopsis has at least ten recognized CuAO genes, however, only five of them ( AtAO1 , AtCuAO1 , AtCuAO2 , AtCuAO3 , and AtCuAO8 ) have been characterized at protein level (Møller and McPherson, 1998; Planas-Portell et al., 2013; Ghuge et al., 2015; Groβ et al., 2017). The apple genome contains five putative CuAO genes with two of them ( MdAO1 and MdAO2 ) being identified at protein level (Zarei et al., 2015) and, recently, eight putative CuAO genes were reported in sweet orange (Wang et al., 2017).…”

Section: Advance In Polyamine Catabolism Researchmentioning

confidence: 99%

Polyamine Catabolism in Plants: A Universal Process With Diverse Functions

et al. 2019

View full text Add to dashboard Cite

Polyamine (PA) catabolic processes are performed by copper-containing amine oxidases (CuAOs) and flavin-containing PA oxidases (PAOs). So far, several CuAOs and PAOs have been identified in many plant species. These enzymes exhibit different subcellular localization, substrate specificity, and functional diversity. Since PAs are involved in numerous physiological processes, considerable efforts have been made to explore the functions of plant CuAOs and PAOs during the recent decades. The stress signal transduction pathways usually lead to increase of the intracellular PA levels, which are apoplastically secreted and oxidized by CuAOs and PAOs, with parallel production of hydrogen peroxide (H 2 O 2 ). Depending on the levels of the generated H 2 O 2 , high or low, respectively, either programmed cell death (PCD) occurs or H 2 O 2 is efficiently scavenged by enzymatic/nonenzymatic antioxidant factors that help plants coping with abiotic stress, recruiting different defense mechanisms, as compared to biotic stress. Amine and PA oxidases act further as PA back-converters in peroxisomes, also generating H 2 O 2 , possibly by activating Ca 2+ permeable channels. Here, the new research data are discussed on the interconnection of PA catabolism with the derived H 2 O 2 , together with their signaling roles in developmental processes, such as fruit ripening, senescence, and biotic/abiotic stress reactions, in an effort to elucidate the mechanisms involved in crop adaptation/survival to adverse environmental conditions and to pathogenic infections.

show abstract

Copper amine oxidase 8 regulates arginine-dependent nitric oxide production in Arabidopsis thaliana

Abstract: HighlightCopper amine oxidase in polyamine metabolism modulates arginine-dependent nitric oxide production by affecting arginase activity in Arabidopsis thaliana.

Cited by 54 publications

References 67 publications

Transcriptome profiling at osmotic and ionic phases of salt stress response in bread wheat uncovers trait-specific candidate genes

Transcriptome profiling at osmotic and ionic phases of salt stress response in bread wheat uncovers trait-specific candidate genes

Isoprene Acts as a Signaling Molecule in Gene Networks Important for Stress Responses and Plant Growth

Polyamine Catabolism in Plants: A Universal Process With Diverse Functions

Contact Info

Product

Resources

About