Copper and molybdenum absorption by rats given ammonium tetrathiomolybdate

Mills, Colin F.; El-Gallad, T.T.; Bremner, I.; Wenham, Gordon J.

doi:10.1016/s0162-0134(00)80037-9

Cited by 74 publications

(26 citation statements)

References 7 publications

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“…4). (Dick et al 1975) and is also the most potent of the TM species in inhibiting Cu absorption (Mills et al 1981;Suttle and Field 1983) and in producing systemic effects (Mason 1986 (Henkin 1974), whereas administration of corticosteroids to sheep (Caple and Heath 1978) and rats (Evans 1973) results in an increase in bile Cu. In this connection, it appears that the effect of corticosteroids on bile Cu excretion in bovine is similar to that of the cat but not sheep and the rat.…”

Section: Plasma Cumentioning

confidence: 99%

Effects of dietary copper, molybdenum and sulfur on biliary copper and zinc excretion in Simmental and Angus cattle

Gooneratne¹,

Christensen²,

Bailey³

et al. 1994

Can. J. Anim. Sci.

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Section: Plasma Cumentioning

confidence: 99%

Effects of dietary copper, molybdenum and sulfur on biliary copper and zinc excretion in Simmental and Angus cattle

Gooneratne¹,

Christensen²,

Bailey³

et al. 1994

Can. J. Anim. Sci.

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“…The mechanism of action of TM involves forming a stable tripartite complex with copper and protein that is unavailable for cellular uptake (Mills et al, 1981). Given with food, TM binds copper in food and endogenously secreted copper with protein in the alimentary tract, and it prevents copper absorption.…”

mentioning

confidence: 99%

Tetrathiomolybdate Protects against Bile Duct Ligation-Induced Cholestatic Liver Injury and Fibrosis

Song¹,

Song²,

Barve³

et al. 2008

J Pharmacol Exp Ther

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“…On the other hand, El Gallad et al (1983) maintain that localized high ratios of su1phide:Mo in the rumen, and therefore TTM formation, cannot be ruled out. The controversy over the presence or absence of the tetra-species is significant, because this is the only form that has been shown to affect rats and sheep to the extent of inducing signs of Cu deficiency or of decreasing Cu absorption (Mills et al 1981 ;Bremner et al 1982; Suttle & Field, 1983). Dithiomolybdate was not effective in either species, and trithiomolybdate was ineffective in rats, but has not been tested in sheep.…”

mentioning

confidence: 99%

Involvement of the solid phase of rumen digesta in the interaction between copper, molybdenum and sulphur in sheep

Allen

Gawthorne

1987

Br J Nutr

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1. Merino sheep fed on a diet of chopped wheaten hay, chopped lucerne (Medicago saliva) hay and oat grain were the source of rumen contents for the study. The diet contained (mg/kg dry weight) 3.3 copper, 024 molybdenum and 2-8 sulphur. The effects of adding between 5 and 25 mg Mo/kg as ammonium molybdate (AM) or tetrathiomolybdate (TTM) on the distribution and forms of Cu and Mo in rumen contents were investigated in vivo and in vitro. 2.Approximately 88 YO of the Cu and 94% of the Mo in rumen contents were associated with the solid phase.When AM or TTM was added to rumen contents in vivo or in vitro the proportion of these elements in the solid phase was increased at the expense of the fluid phase.3. The addition of AM and TTM to rumen contents also decreased the proportion of Cu that was soluble in trichloroacetic acid (50 g/l ; TCA) and increased the proportion of Cu that was not extractable by sequential treatment with TCA and neutral detergent.4. Column chromatography of neutral-detergent extracts of rumen contents revealed that TTM treatment caused Cu to be strongly bound to proteins of high molecular weight.5. Addition of sulphide to rumen contents did not result in significant changes in the distribution of Cu between the fluid and solid phases, or in the solubility of Cu in TCA.6. It is postulated that constant removal of TTM from the fluid phase via reaction with proteins and other macromolecules in the solid phase results in greater formation of TTM in vivo than would be expected from solution chemistry. The molybdo-proteins so formed are strong chelators of Cu and may be the agents responsible for the decrease in Cu absorption in animals that consume diets containing high concentrations of Mo.When relatively high concentrations of molybdenum and sulphur are simultaneously present in the diet of ruminants the availability of copper to the animal is seriously compromised (Dick, 1956). The production of thiomolybdates in the rumen, and the subsequent decrease in the availability of Cu due to the formation of insoluble Cu thiomolybdates has been suggested as a hypothesis for the interaction (Dick et al. 1975). Confirmation of the hypothesis via spectrophotometric evidence of the presence of these compounds in rumen contents has been hampered by interference from other compounds, and by the low concentrations of Mo normally present. However, at moderately high molybdate concentrations, absorption spectra typical of thiomolybdates have been found in washed suspensions of rumen micro-organisms (Dick et al. 1975;El Gallad et al. 1983), in an artificial rumen apparatus (Bray et al. 1982), and in whole rumen contents (El Gallad et al. 1983), thereby indicating that the potential for thiomolybdate formation does exist .Interest has also centred on the particular species of thiomolybdates that may occur in vivo. Based on observations of solution chemistry, Clarke 8z Laurie (1980) concluded that tetrathiomolybdate (TTM) would not form unless the sulphide : Mo ratio was very high, and that at the lower ratios mor...

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Copper and molybdenum absorption by rats given ammonium tetrathiomolybdate

Cited by 74 publications

References 7 publications

Effects of dietary copper, molybdenum and sulfur on biliary copper and zinc excretion in Simmental and Angus cattle

Effects of dietary copper, molybdenum and sulfur on biliary copper and zinc excretion in Simmental and Angus cattle

Tetrathiomolybdate Protects against Bile Duct Ligation-Induced Cholestatic Liver Injury and Fibrosis

Involvement of the solid phase of rumen digesta in the interaction between copper, molybdenum and sulphur in sheep

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