Copper and zinc impact on stress biomarkers and growth parameters in a model organism, Galleria mellonella larvae

Coşkun, Mustafa; Kayış, Tamer; Yılmaz, Mehmet; Dursun, Osman; Emre, İskender

doi:10.1007/s10534-021-00341-w

Cited by 14 publications

(3 citation statements)

References 66 publications

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“…The massive influence of CuSO 4 exposure on the larval development and survival rate of Galleria mellonella due to oxidative stress has already been described. For example, it has been verified that CuSO 4 is a source of oxidative stress for invertebrate models [100,101]. Tuncsoy et al [102] also observed a modulation on the antioxidant enzyme activity for G. mellonella larvae exposed to copper oxide nanoparticles.…”

Section: Discussionmentioning

confidence: 98%

Exploring the Antioxidant Potential of Talisia esculenta Using In Vitro and In Vivo Approaches

da Silva Cordeiro,

de Queiroz Aquino-Martins,

da Silva

et al. 2023

Nutrients

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Medicinal plants, such as Talisia esculenta, are rich in antioxidant biomolecules, which are used in the treatment and prevention of many diseases. The antioxidant potential of T. esculenta extracts obtained from leaves and fruit peels was investigated using biochemical and 3T3 cell line assays as well as in vivo assays using an organism model Tenebrio molitor. Four extracts were tested: hydroethanolic extracts from leaves (HF) and from fruit peels (HC), and infusion extracts from leaves (IF) and from fruit peels (IC). The biochemical assays demonstrated an antioxidant capacity verified by TAC, reducing power, DPPH, and copper chelating assays. None of the extracts exhibited cytotoxicity against 3T3 cells, instead offering a protection against CuSO4-induced oxidative stress. The antioxidant activity observed in the extracts, including their role as free radical scavengers, copper chelators, and stress protectors, was further confirmed by T. molitor assays. The CLAE-DAD analysis detected phenolic compounds, including gallic acid, rutin, and quercitrin, as the main constituents of the samples. This study highlights that leaf and fruit peels extracts of T. esculenta could be effective protectors against ROS and copper-induced stress in cellular and invertebrate models, and they should be considered as coadjutants in the treatment and prevention of diseases related to oxidative stress and for the development of natural nutraceutical products.

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Section: Discussionmentioning

confidence: 98%

Exploring the Antioxidant Potential of Talisia esculenta Using In Vitro and In Vivo Approaches

da Silva Cordeiro,

de Queiroz Aquino-Martins,

da Silva

et al. 2023

Nutrients

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“…The levels of circulating hemocytes and PO enzyme activity are generally very sensitive biological parameters for metal concentrations [ 161 ]. Essential and nonessential elements play dual roles in reproduction and immunity in insects [ 162 , 163 ]. For example, the exposure of grasshoppers to a high concentration of Zn (100–1000 µg g −1 ) during diapause causes a high frequency of apoptosis and necrosis in adult tissues and the aging of females evident in eggs laid, and also affects the duration of embryogenesis [ 164 ].…”

Section: Discussionmentioning

confidence: 99%

Triazine Herbicide and NPK Fertilizer Exposure: Accumulation of Heavy Metals and Rare Earth Elements, Effects on Cuticle Melanization, and Immunocompetence in the Model Species Tenebrio molitor

Naccarato

Vommaro

Amico

et al. 2023

Toxics

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The increasing use of agrochemicals, including fertilizers and herbicides, has led to worrying metal contamination of soils and waters and raises serious questions about the effects of their transfer to different levels of the trophic web. Accumulation and biomagnification of essential (K, Na, Mg, Zn, Ca), nonessential (Sr, Hg, Rb, Ba, Se, Cd, Cr, Pb, As), and rare earth elements (REEs) were investigated in newly emerged adults of Tenebrio molitor exposed to field-admitted concentrations of a metribuzin-based herbicide and an NPK blend fertilizer. Chemical analyses were performed using inductively coupled plasma tandem mass spectrometry (ICP-MS/MS) supported by unsupervised pattern recognition techniques. Physiological parameters such as cuticle melanization, cellular (circulating hemocytes), and humoral (phenoloxidase enzyme activity) immune responses and mass loss were tested as exposure markers in both sexes. The results showed that NPK fertilizer application is the main cause of REE accumulation in beetles over time, besides toxic elements (Sr, Hg, Cr, Rb, Ba, Ni, Al, V, U) also present in the herbicide-treated beetles. The biomagnification of Cu and Zn suggested a high potential for food web transfer in agroecosystems. Gender differences in element concentrations suggested that males and females differ in element uptake and excretion. Differences in phenotypic traits show that exposure affects metabolic pathways involving sequestration and detoxification during the transition phase from immature-to-mature beetles, triggering a redistribution of resources between sexual maturation and immune responses. Our findings highlight the importance of setting limits for metals and REEs in herbicides and fertilizers to avoid adverse effects on species that provide ecosystem services and contribute to soil health in agroecosystems.

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“…Many studies indicated that heavy metals would promote the formation of ROS (reactive oxygen species) [ 27 , 28 , 29 ]. The balance between production and elimination of ROS is kept under normal circumstances, in which extra heavy metals would strike due to the variation in antioxidant enzyme activities [ 30 ]. Cd influenced antioxidant enzyme activity under long-term stress in our study, which supports the results in previous research that showed changes in the activity of SOD, POD, CAT and GST.…”

Section: Discussionmentioning

confidence: 99%

Effects of Long-Term Exposure to Cadmium on Development, Reproduction and Antioxidant Enzymes of Aleuroglyphus ovatus (Acari: Acaridae)

Xiong

Yang

et al. 2022

Insects

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Grain contaminated by cadmium (Cd) has become a serious food security problem, and it is necessary to determine and evaluate the toxic effect and defense mechanism of long-term heavy metal pollution in grain. In order to evaluate the effects of long-term heavy metal Cd stress on the stored grain pests, Aleuroglyphus ovatus were fed with an artificial diet supplemented with different concentrations of Cd (0, 5, 10, 20 mg/kg). The development, fecundity and detoxification enzymes of A. ovatus were analyzed and observed. In this study, the immature duration of A. ovatus was significantly prolonged under long-term Cd stress. Moreover, the survival duration of female adults was significantly shortened. The total number of eggs laid and the daily number laid per female adult decreased significantly. There were significant differences in protein content at protonymph and tritonymph stages when the concentration of Cd exceeded 10 mg/kg. The protein content of female adults was higher than that of male adults. The activity of detoxification enzymes showed differences in different conditions, such as development stage, Cd concentration and gender. These findings confirmed that A. ovatus were sensitive to Cd, and their offspring were severely affected under long-term Cd stress. Therefore, A. ovatus is a good model for evaluating the toxicity of long-term heavy metal Cd stress. The study provides the basis and enriches the research content of heavy metal pollution on mites, contributing to the harmonious and healthy development between the environment and human beings.

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Copper and zinc impact on stress biomarkers and growth parameters in a model organism, Galleria mellonella larvae

Cited by 14 publications

References 66 publications

Exploring the Antioxidant Potential of Talisia esculenta Using In Vitro and In Vivo Approaches

Exploring the Antioxidant Potential of Talisia esculenta Using In Vitro and In Vivo Approaches

Triazine Herbicide and NPK Fertilizer Exposure: Accumulation of Heavy Metals and Rare Earth Elements, Effects on Cuticle Melanization, and Immunocompetence in the Model Species Tenebrio molitor

Effects of Long-Term Exposure to Cadmium on Development, Reproduction and Antioxidant Enzymes of Aleuroglyphus ovatus (Acari: Acaridae)

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