Copper excess promotes propagation and induces proteomic change in root cultures of Hyoscyamus albus L.

Abstract

Hyoscyamus albus L. seedlings respond positively to copper (Cu) excess. In the present study, to understand how roots cope with Cu excess, propagation and proteome composition in the presence of Cu were examined using a root culture system. When H. albus roots were cultured in a medium without Cu, root growth deteriorated. However, in the presence of Cu, root growth increased in a concentration-dependent manner, and vigorous lateral root development was observed at 200 μM Cu. Cu accumulation in the roots increased with the Cu supply. Subcellular fractionation revealed that the highest amount of Cu was present in the cell wall-containing fraction, followed by the soluble fraction. However, the highest specific incorporation of Cu, in terms of fresh weight, was in the mitochondria-rich fraction. High Cu levels enhanced respiration activity. Comparative proteomic analysis revealed that proteins involved in carbohydrate metabolism, de novo protein synthesis, cell division, and ATP synthesis increased in abundance, whereas the proteasome decreased. These results indicate that Cu promotes propagation of H. albus roots through the activation of the energy supply and anabolism. Newly propagated root tissues and newly generated proteins that bind to Cu may provide space and reservoirs for deposition of additional Cu.

Can pesticides, copper and seasonal water temperature explain the seagrass Zostera noltei decline in the Arcachon bay?

“…Indeed, the number of mitochondria (12S gene) as well as the expression levels of cox1 (complex IV of the mitochondrial respiratory chain, located on the inner membrane of mitochondria) and nad5 (complex I) increased, probably to maintain a sufficient pool of ATP and viability of cells. This result was in agreement with recent report of Sako et al (2016) where Cu was demonstrated to highly accumulated in mitochondria of the plant Hyoscyamus albus. Moreover, they evidenced that high Cu levels enhanced respiration activity and comparative proteomic analysis revealed that proteins involved in carbohydrate metabolism and ATP synthesis increased in abundance..…”

“…Contamination with the pesticide cocktail led to an increase in mitochondria (12S gene), and an overexpression of cox1 and nad5 genes at 28 °C. This would allow maintaining a sufficient pool ATP and cells viability, offsetting the deleterious effect of the contaminant (Sako et al 2016). Moreover, 20% mortality was observed at the same temperature after 14 days exposure and correlates with the reduced growth induced by the pesticide.…”

“…All these cellular functions were chosen to investigate the molecular impacts on mitochondria and chloroplasts which were previously described as putative primary target of pesticides and Cu in cells (Sako et al, 2016;Gomes et al, 2017). Results obtained during this experimental approach were completed by a field survey, from March to July 2014, of the health status of Arcahon Bay eelgrass.…”