Copper(II)-neocuproine as colour reagent for some biologically active thiols: Spectrophotometric determination of cysteine, penicillamine, glutathione, and 6-mercaptopurine

Besada, Amir; Tadros, Nabil B.; Gawargious, Y. A.

doi:10.1007/bf01242800

Cited by 47 publications

(24 citation statements)

References 6 publications

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“…[9]. GSH can now be detected by some methods such as photonic [10], high performance liquid chromatography (HPLC) [11][12][13], spectrofluorimetry [14], spectrophotometry [15,16], electrochemistry [17], and capillary electrophoresis combined with electrochemical detection [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Novel sensor based on carbon paste/Nafion® modified with gold nanoparticles for the determination of glutathione

2012

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Several problems for the direct electrochemical oxidation of reduced glutathione (GSH) challenge the usage of electroanalytical techniques for its determination. In this work, the electrochemical oxidation of GSH catalyzed by gold nanoparticles electrodeposited on Nafion modified carbon paste electrode in 0.04 mol L(-1) universal buffer solution (pH 7.4) is proved successful. The effect of various experimental parameters including pH, scan rate and stability on the voltammetric response of GSH was investigated. At the optimum conditions, the concentration of GSH was determined using differential pulse voltammetry (DPV) in two concentration ranges: 0.1 × 10(-7) to 1.6 × 10(-5) mol L(-1) and 2.0 × 10(-5) to 2.0 × 10(-4) mol L(-1) with correlation coefficients 0.9988, 0.9949 and the limit of detections (LOD) are 3.9 × 10(-9) mol L(-1) and 8.2 × 10(-8) mol L(-1), respectively, which confirmed the sensitivity of the electrode. The high sensitivity, wide linear range, good stability and reproducibility, and the minimal surface fouling make this modified electrode useful for the determination of spiked GSH in urine samples and in tablet with excellent recovery results obtained.

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Section: Introductionmentioning

confidence: 99%

Novel sensor based on carbon paste/Nafion® modified with gold nanoparticles for the determination of glutathione

2012

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“…27 The molar ratio of neocuprione and Cu II in the reaction mixture was determined in the optimization part of the experiment. According to these results the copper(II)-neocuproine reagent was prepared as follows: 25.0 mg of copper sulphate pentahydrate and 50.0 mg of neocuproine were dissolved in the 100.0 mL of deionised water.…”

Section: Methodsmentioning

confidence: 99%

Kinetic Spectrophotometric Determination of N-acetyl-L-cysteine Based on the Reduction of Copper(II)-neocuproine Reagent

Radić¹,

Kukoc-Modun²,

Biocic³

2013

Croat. Chem. Acta

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“…The conventional methods [14][15][16][17], and amperometric determination following liquid chromatography [18] were reproted for cysteine and glutathione. The electrocatalytic oxidation of cysteine at a phthalocyanine tetrasulfonate mediated graphite electrode was reported by Zagal et al, [19].…”

Section: Introductionmentioning

confidence: 99%

Electrocatalytic Oxidation of Sulfhydryl Compounds at Ruthenium(III) Diphenyldithiocarbamate Modified Carbon Paste Electrode

Nalini

Narayanan

1998

Electroanalysis

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An aqueous insoluble new redox mediator ruthenium(III)-diphenyldithiocarbamate has been used as a modifier to prepare a carbon paste electrode. The modified electrode was characterized by cyclic voltammetry by varying the pH of the medium and also the scan rate. The electrode was scanned between 0.0 V and þ0.8 V in 0.1 M KNO 3 medium. Anodic and cathodic peaks were observed at þ0.38 V and þ0.22 V, respectively, for a scan rate of 100 mV/s. The modified electrode is found to oxidize electrocatalytically cysteine and glutathione. Conditions such as pH and scan rate have been optimized for the determination of both compounds. Linear response for cysteine is in the range of 0.00-15.20 mg/L, with a correlation coefficient (r), of 0.9992. Standard deviation for 10 repetitive determinations was found to be 0.0756. The linear range for glutathione is 0.00-30.40 mg/L, with a value of 0.9995 for r, and the standard deviation is 0.1449. The stability and reproducibility of the electrode for the determination of cysteine and glutathione were also discussed. Hydrodynamic voltammogram was also recorded for the electrocatalytic oxidation of the sulfhydryl compounds. Amperometric determination and studies on the stability of the electrode for a period of 10 days were also carried out.

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Copper(II)-neocuproine as colour reagent for some biologically active thiols: Spectrophotometric determination of cysteine, penicillamine, glutathione, and 6-mercaptopurine

Cited by 47 publications

References 6 publications

Novel sensor based on carbon paste/Nafion® modified with gold nanoparticles for the determination of glutathione

Novel sensor based on carbon paste/Nafion® modified with gold nanoparticles for the determination of glutathione

Kinetic Spectrophotometric Determination of N-acetyl-L-cysteine Based on the Reduction of Copper(II)-neocuproine Reagent

Electrocatalytic Oxidation of Sulfhydryl Compounds at Ruthenium(III) Diphenyldithiocarbamate Modified Carbon Paste Electrode

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