Copper-induced intracellular calcium release requires extracellular calcium entry and activation of L-type voltage-dependent calcium channels in Ulva compressa

González, Alberto; Cabrera, Maria de; Mellado, Macarena; Cabello, Susana; Márquez, Seabastián; Morales, Bernardo; Moenne, Alejandra

doi:10.4161/psb.20355

Cited by 19 publications

(21 citation statements)

References 23 publications

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“…However, in another study, copper excess rapidly increased in vivo DAG accumulation in roots of B. napus that could reflect PI-PLC activation [131]. Moreover, copper-induced release of intracellular calcium in marine alga Ulvia compressa seems to be dependent on PI-PLC activity [132].…”

Section: Heavy Metal Stressmentioning

confidence: 88%

Plant phosphoinositide-dependent phospholipases C: Variations around a canonical theme

et al. 2014

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Phosphoinositide-specific phospholipase C (PI-PLC) cleaves, in a Ca(2+)-dependent manner, phosphatidylinositol-4,5-bisphosphate (PI-4,5-P2) into diacylglycerol (DAG) and inositol triphosphate (IP3). PI-PLCs are multidomain proteins that are structurally related to the PI-PLCζs, the simplest animal PI-PLCs. Like these animal counterparts, they are only composed of EF-hand, X/Y and C2 domains. However, plant PI-PLCs do not have a conventional EF-hand domain since they are often truncated, while some PI-PLCs have no EF-hand domain at all. Despite this simple structure, plant PI-PLCs are involved in many essential plant processes, either associated with development or in response to environmental stresses. The action of PI-PLCs relies on the mediators they produce. In plants, IP3 does not seem to be the sole active soluble molecule. Inositol pentakisphosphate (IP5) and inositol hexakisphosphate (IP6) also transmit signals, thus highlighting the importance of coupling PI-PLC action with inositol-phosphate kinases and phosphatases. PI-PLCs also produce a lipid molecule, but plant PI-PLC pathways show a peculiarity in that the active lipid does not appear to be DAG but its phosphorylated form, phosphatidic acid (PA). Besides, PI-PLCs can also act by altering their substrate levels. Taken together, plant PI-PLCs show functional differences when compared to their animal counterparts. However, they act on similar general signalling pathways including calcium homeostasis and cell phosphoproteome. Several important questions remain unanswered. The cross-talk between the soluble and lipid mediators generated by plant PI-PLCs is not understood and how the coupling between PI-PLCs and inositol-kinases or DAG-kinases is carried out remains to be established.

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Section: Heavy Metal Stressmentioning

confidence: 88%

Plant phosphoinositide-dependent phospholipases C: Variations around a canonical theme

et al. 2014

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“…As a secondary messenger of signal transduction within the cell of algae, Ca 2+ was closely related to stress adjustment mechanism of algae cell (Maksymiec 2007). Studies have shown that Ca 2+ permeable cationic channel of the algae had been affected by different types and concentrations of heavy metal ions (González et al 2012;Batistič and Kudla 2010). The reason for this may be that Ca 2+ combined with receptor within the algae to resist the low concentration heavy metal stress (Boyd 2010).…”

Section: Effects Of Cu 2+ and Zn 2+ On The Calcium Transduction Of CLmentioning

confidence: 96%

“…In marine algae, it has been shown that Ulva compressa (Chlorophyta) exposed to 10 M copper showed a triphasic increase of intracellular calcium with maximal levels (González et al 2012). As a secondary messenger of signal transduction within the cell of algae, Ca 2+ was closely related to stress adjustment mechanism of algae cell (Maksymiec 2007).…”

Section: Effects Of Cu 2+ and Zn 2+ On The Calcium Transduction Of CLmentioning

confidence: 99%

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Effects of Cu2+ and Zn2+ on growth and physiological characteristics of green algae, Cladophora

Cao

Xie

Deng

et al. 2015

Environ Sci Pollut Res

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Effects of various concentrations of Cu(2+) and Zn(2+) (0.0, 0.1, 0.25, 0.5, or 1.0 mg/L) on the growth, malondialdehyde (MDA), the intracellular calcium, and physiological characteristics of green algae, Cladophora, were investigated. Low Zn(2+) concentrations accelerated the growth of Cladophora, whereas Zn(2+) concentration increases to 0.25 mg/L inhibited its growth. Cu(2+) greatly influences Cladophora growth. The photosynthesis of Cladophora decreased under Zn(2+) and Cu(2+) stress. Cu(2+) and Zn(2+) treatment affected the content of total soluble sugar in Cladophora and has small increases in its protein content. Zn(2+) induced the intracellular calcium release, and copper induced the intracellular calcium increases in Cladophora. Exposure to Cu(2+) and Zn(2+) induces MDA in Cladophora. The stress concent of Cu(2+) was strictly correlated with the total soluble sugar content, Chla+Chlb, and MDA in Cladophora, and the stress concent of Zn(2+) was strictly correlated with the relative growth rate (RGR) and MDA of Cladophora.

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“…With respect to biochemical responses to Cu stress, the most comprehensive studies, to date, have been on the green seaweed Ulva compressa (Ulvophyceae) and brown seaweed Scytosiphon lomentaria (Phaeophyceae), two prominent members of the seaweed community in Cu-enriched coastal waters (over 1000 g L −1 ) of northern Chile (Lee and Correa, 2005;Ramirez et al, 2005). Chronic exposure of U. compressa to Cu induced an oxidative stress condition buffered mainly through the activation of the antioxidant enzyme APX, and synthesis of ASC whereas in S. lomentaria protection from oxidative stress occurs via increased activities of mainly CAT, GP and APX, and production of ASC (Contreras et al, 2005(Contreras et al, , 2009González et al, 2012b;Mellado et al, 2012;Ratkevicius et al, 2003). In both these species it was concluded that tolerance to Cu is a constitutive trait (Contreras et al, 2005;Ratkevicius et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Copper-induced intra-specific oxidative damage and antioxidant responses in strains of the brown alga Ectocarpus siliculosus with different pollution histories

et al. 2015

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Inter- and intra-specific variation in metal resistance has been observed in the ecologically and economically important marine brown macroalgae (Phaeophyceae), but the mechanisms of cellular tolerance are not well elucidated. To investigate inter-population responses of brown seaweeds to copper (Cu) pollution, the extent of oxidative damage and antioxidant responses were compared in three strains of the filamentous brown seaweed Ectocarpus siliculosus, the model organism for the algal class Phaeophyceae that diverged from other major eukaryotic groups over a billion year ago. Strains isolated from locations with different pollution histories (i.e. LIA, from a pristine site in Scotland; REP and Es524 from Cu-contaminated sites in England and Chile, respectively) were exposed to total dissolved Cu concentrations (CuT) of up to 2.4 μM (equivalent to 128 nM Cu(2+)) for 10 d. LIA exhibited oxidative stress, with increases in hydrogen peroxide (H2O2) and lipid peroxidation (measured as TBARS levels), and decreased concentrations of photosynthetic pigments. Es524 presented no apparent oxidative damage whereas in REP, TBARS increased, revealing some level of oxidative damage. Adjustments to activities of enzymes and antioxidant compounds concentrations in Es524 and REP were strain and treatment dependent. Mitigation of oxidative stress in Es524 was by increased activities of superoxide dismutases (SOD) at low CuT, and catalase (CAT) and ascorbate peroxidase (APX) at all CuT, accompanied by higher levels of antioxidants (ascorbate, glutathione, phenolics) at higher CuT. In REP, only APX activity increased, as did the antioxidants. For the first time evidence is presented for distinctive oxidative stress defences under excess Cu in two populations of a species of brown seaweed from environments contaminated by Cu.

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Copper-induced intracellular calcium release requires extracellular calcium entry and activation of L-type voltage-dependent calcium channels in Ulva compressa

Cited by 19 publications

References 23 publications

Plant phosphoinositide-dependent phospholipases C: Variations around a canonical theme

Plant phosphoinositide-dependent phospholipases C: Variations around a canonical theme

Effects of Cu2+ and Zn2+ on growth and physiological characteristics of green algae, Cladophora

Copper-induced intra-specific oxidative damage and antioxidant responses in strains of the brown alga Ectocarpus siliculosus with different pollution histories

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