Copper-induced metabolic variation of oysters overwhelmed by salinity effects

Cao, Chen; Wang, Wen‐Xiong

doi:10.1016/j.chemosphere.2017.01.150

Cited by 21 publications

(12 citation statements)

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“…As branched chain amino acids like leucine are involved in haemocyanin synthesis and transportation [50], the increased level of leucine found in the present study may be linked to a decrease of the respiratory protein upon copper exposure. This trend was observed by Cao and collaborators when studying the combined effect of salinity and copper exposure in oysters [36]: An increased level of leucine was observed in 15 g/kg seawater plus Cu compared with 10 g/kg plus Cu waters.…”

Section: Energy Metabolism and Oxygen Transportsupporting

confidence: 61%

“…Such a decrease is indeed observed in this case, which would therefore confirm the use of anaerobic metabolism by scallops exposed to Cu. Cao and collaborators also observed a decrease in several amino acids including aspartate, when studying copper-induced metabolic variation in oysters [36]. They attributed this phenomenon to a mobilization of amino acids to produce energy for maintaining function of pathways in these Cu-exposed organisms.…”

Section: Energy Metabolism and Oxygen Transportmentioning

confidence: 90%

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Untargeted Metabolomics Reveals a Complex Impact on Different Metabolic Pathways in Scallop Mimachlamys varia (Linnaeus, 1758) after Short-Term Exposure to Copper at Environmental Dose

et al. 2021

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Ports are a good example of how coastal environments, gathering a set of diverse ecosystems, are subjected to pollution factors coming from human activities both on land and at sea. Among them, trace element as copper represents a major factor. Abundant in port ecosystem, copper is transported by runoff water and results from diverse port features (corrosion of structures, fuel, anti-fouling products, etc.). The variegated scallop Mimachlamys varia is common in the Atlantic port areas and is likely to be directly influenced by copper pollution, due to its sessile and filtering lifestyle. Thus, the aim of the present study is to investigate the disruption of the variegated scallop metabolism, under a short exposure (48 h) to a copper concentration frequently encountered in the waters of the largest marina in Europe (82 μg/L). For this, we chose a non-targeted metabolomic approach using ultra-high performance liquid chromatography coupled to high resolution mass spectrometry (UHPLC-HRMS), offering a high level of sensitivity and allowing the study without a priori of the entire metabolome. We described 28 metabolites clearly modulated by copper. They reflected the action of copper on several biological functions such as osmoregulation, oxidative stress, reproduction and energy metabolism.

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Section: Energy Metabolism and Oxygen Transportsupporting

confidence: 61%

Section: Energy Metabolism and Oxygen Transportmentioning

confidence: 90%

Untargeted Metabolomics Reveals a Complex Impact on Different Metabolic Pathways in Scallop Mimachlamys varia (Linnaeus, 1758) after Short-Term Exposure to Copper at Environmental Dose

et al. 2021

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“…It has been reported that glycogen levels are massively reduced under hypo-osmotic stress [ 53 ], and that when the glycogen is exhausted, the osmotic imbalance caused by salinity stress is more severe [ 54 ]. It has also been reported that glycogen could be a potential biomarker for Cu-induced toxic effects in the Pacific oyster [ 55 ]. Although it has been widely recognized that glycogen participates in stress resistance in the oyster, the molecular mechanism behind this has not been identified.…”

Section: Discussionmentioning

confidence: 99%

Integrated application of transcriptomics and metabolomics provides insights into glycogen content regulation in the Pacific oyster Crassostrea gigas

Song

Meng

et al. 2017

BMC Genomics

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BackgroundThe Pacific oyster Crassostrea gigas is an important marine fishery resource, which contains high levels of glycogen that contributes to the flavor and the quality of the oyster. However, little is known about the molecular and chemical mechanisms underlying glycogen content differences in Pacific oysters. Using a homogeneous cultured Pacific oyster family, we explored these regulatory networks at the level of the metabolome and the transcriptome.ResultsOysters with the highest and lowest natural glycogen content were selected for differential transcriptome and metabolome analysis. We identified 1888 differentially-expressed genes, seventy-five differentially-abundant metabolites, which are part of twenty-seven signaling pathways that were enriched using an integrated analysis of the interaction between the differentially-expressed genes and the differentially-abundant metabolites. Based on these results, we found that a high expression of carnitine O-palmitoyltransferase 2 (CPT2), indicative of increased fatty acid degradation, is associated with a lower glycogen content. Together, a high level of expression of phosphoenolpyruvate carboxykinase (PEPCK), and high levels of glucogenic amino acids likely underlie the increased glycogen production in high-glycogen oysters. In addition, the higher levels of the glycolytic enzymes hexokinase (HK) and pyruvate kinase (PK), as well as of the TCA cycle enzymes malate dehydrogenase (MDH) and pyruvate carboxylase (PYC), imply that there is a concomitant up-regulation of energy metabolism in high-glycogen oysters. High-glycogen oysters also appeared to have an increased ability to cope with stress, since the levels of the antioxidant glutathione peroxidase enzyme 5 (GPX5) gene were also increased.ConclusionOur results suggest that amino acids and free fatty acids are closely related to glycogen content in oysters. In addition, oysters with a high glycogen content have a greater energy production capacity and a greater ability to cope with stress. These findings will not only provide insights into the molecular mechanisms underlying oyster quality, but also promote research into the molecular breeding of oysters.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-4069-8) contains supplementary material, which is available to authorized users.

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“…gigas. Glycogen is essential to the reproduction and stress response , of oysters, and it is the main molecular contributor to flavor and other critical quality traits. − However, the molecular mechanisms of glycogenin activity in mollusks have not been reported. In the present study, we identified a gene encoding glycogenin in C.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, glucose polymerization is a universal mechanism for energy storage in nature. For the Pacific oyster ( Crassostrea gigas ), a worldwide cultivated marine species, glycogen is closely related to reproduction, stress response, , and gonadal development. , Additionally, glycogen content and fatty acid content strongly affects oyster flavor; thus, both are critical to oyster quality. A previous study has described the polyunsaturated fatty acid (PUFA) biosynthesis pathway in noble scallops ( Chlamys nobilis ), and several in vivo , and in vitro studies had been conducted to investigate glycogen metabolisms in oysters.…”

Section: Introductionmentioning

confidence: 99%

Identification and Functional Characterization of the Glycogen Synthesis Related Gene Glycogenin in Pacific Oysters (Crassostrea gigas)

Meng

et al. 2017

J. Agric. Food Chem.

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High glycogen levels in the Pacific oyster (Crassostrea gigas) contribute to its flavor, quality, and hardiness. Glycogenin (CgGN) is the priming glucosyltransferase that initiates glycogen biosynthesis. We characterized the full sequence and function of C. gigas CgGN. Three CgGN isoforms (CgGN-α, β, and γ) containing alternative exon regions were isolated. CgGN expression varied seasonally in the adductor muscle and gonadal area and was the highest in the adductor muscle. Autoglycosylation of CgGN can interact with glycogen synthase (CgGS) to complete glycogen synthesis. Subcellular localization analysis showed that CgGN isoforms and CgGS were located in the cytoplasm. Additionally, a site-directed mutagenesis experiment revealed that the Tyr200Phe and Tyr202Phe mutations could affect CgGN autoglycosylation. This is the first study of glycogenin function in marine bivalves. These findings will improve our understanding of glycogen synthesis and accumulation mechanisms in mollusks. The data are potentially useful for breeding high-glycogen oysters.

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Copper-induced metabolic variation of oysters overwhelmed by salinity effects

Cited by 21 publications

References 50 publications

Untargeted Metabolomics Reveals a Complex Impact on Different Metabolic Pathways in Scallop Mimachlamys varia (Linnaeus, 1758) after Short-Term Exposure to Copper at Environmental Dose

Untargeted Metabolomics Reveals a Complex Impact on Different Metabolic Pathways in Scallop Mimachlamys varia (Linnaeus, 1758) after Short-Term Exposure to Copper at Environmental Dose

Integrated application of transcriptomics and metabolomics provides insights into glycogen content regulation in the Pacific oyster Crassostrea gigas

Identification and Functional Characterization of the Glycogen Synthesis Related Gene Glycogenin in Pacific Oysters (Crassostrea gigas)

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