Copper-induced overexpression of genes encoding antioxidant system enzymes and metallothioneins involve the activation of CaMs, CDPKs and MEK1/2 in the marine alga Ulva compressa

Laporte, Daniel; Valdés, Natalia; González, Alberto; Sáez, Claudio A.; Zúñiga, Antonio; Navarrete, Axel; Meneses, Claudio; Moenne, Alejandra

doi:10.1016/j.aquatox.2016.06.017

Cited by 34 publications

(30 citation statements)

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“…The nano-equivalents of thiol groups present in glutathione and phytochelatins correlated with the level of intracellular accumulated copper [15]. In addition, the level of transcripts of encoding MTs also increased in response to increasing concentration of copper [16]. Thus, cysteine-rich peptides and proteins are involved in copper accumulation and probably participate in the inhibition of copper-induced oxidative stress in U. compressa by sequestering copper ions [12].…”

Section: The Importance Of S Assimilation In U Compressa In Responsementioning

confidence: 99%

“…In addition, U. compressa accumulate copper in its tissue, reaching 620 μg g − 1 when cultivated with 10 μM copper for 12 days [15]. Copper accumulation correlates with the synthesis of GSH, and phytochelatins (PCs), which are peptides produced through condensation of GSH units, and with the increase in expression of metallothioneins (MTs), which are small size cysteine-rich proteins that bind monovalent and divalent metal ions [16]. Initial transcriptomic analyses using RNAseq performed in U. compressa cultivated with 10 μM copper for 0 and 24 h allowed the identification of 7 potential MTs as well as transcripts that encode antioxidant enzymes, and enzymes involved in ASC and GSH synthesis [16].…”

Section: Introductionmentioning

confidence: 99%

“…Copper accumulation correlates with the synthesis of GSH, and phytochelatins (PCs), which are peptides produced through condensation of GSH units, and with the increase in expression of metallothioneins (MTs), which are small size cysteine-rich proteins that bind monovalent and divalent metal ions [16]. Initial transcriptomic analyses using RNAseq performed in U. compressa cultivated with 10 μM copper for 0 and 24 h allowed the identification of 7 potential MTs as well as transcripts that encode antioxidant enzymes, and enzymes involved in ASC and GSH synthesis [16]. The levels of transcripts encoding MTs, antioxidant enzymes, and enzymes involved in ASC and GSH synthesis, were increased in the alga exposed to copper excess [16].…”

Section: Introductionmentioning

confidence: 99%

“…Initial transcriptomic analyses using RNAseq performed in U. compressa cultivated with 10 μM copper for 0 and 24 h allowed the identification of 7 potential MTs as well as transcripts that encode antioxidant enzymes, and enzymes involved in ASC and GSH synthesis [16]. The levels of transcripts encoding MTs, antioxidant enzymes, and enzymes involved in ASC and GSH synthesis, were increased in the alga exposed to copper excess [16]. Finally, transcripts encoding three MTs, MT1, MT2 and MT3, were cloned from U. compressa and their overexpression in bacteria allowed the accumulation of copper and zinc in vivo [17].…”

Section: Introductionmentioning

confidence: 99%

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Copper-induced concomitant increases in photosynthesis, respiration, and C, N and S assimilation revealed by transcriptomic analyses in Ulva compressa (Chlorophyta)

et al. 2020

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Background: The marine alga Ulva compressa is the dominant species in copper-polluted coastal areas in northern Chile. It has been shown that the alga tolerates micromolar concentrations of copper and accumulates copper at the intracellular level. Transcriptomic analyses were performed using total RNA of the alga cultivated with 10 μ M copper for 0, 1, 3 and 5 days using RNA-seq in order to identify processes involved in copper tolerance. Results: The levels of transcripts encoding proteins belonging to Light Harvesting Complex II (LHCII), photosystem II (PSII), cytochrome b6f, PSI, LHCI, ATP synthase and proteins involved in repair of PSII and protection of PSI were increased in the alga cultivated with copper. In addition, the level of transcripts encoding proteins of mitochondrial electron transport chain, ATP synthase, and enzymes involved in C, N and S assimilation were also enhanced. The higher percentages of increase in the level of transcripts were mainly observed at days 3 and 5. In contrast, transcripts involved protein synthesis and degradation, signal transduction, and replication and DNA repair, were decreased. In addition, net photosynthesis and respiration increased in the alga cultivated with copper, mainly at days 1 to 3. Furthermore, the activities of enzymes involved in C, N and S assimilation, rubisco, glutamine synthase and cysteine synthase, respectively, were also increased, mainly at days 1 and 3. Conclusions: The marine alga U. compressa tolerates copper excess through a concomitant increase in expression of proteins involved in photosynthesis, respiration, and C, N and S assimilation, which represents an exceptional mechanism of copper tolerance.

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Section: The Importance Of S Assimilation In U Compressa In Responsementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Copper-induced concomitant increases in photosynthesis, respiration, and C, N and S assimilation revealed by transcriptomic analyses in Ulva compressa (Chlorophyta)

et al. 2020

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“…Thus, it is possible that U. compressa can accumulate copper mediated by MTs. A transcriptomic analysis performed with the alga cultivated with 10 µM copper for 0 and 24 h allowed the identification of 7 potential MTs in U. compressa and their levels increased from days 3 to 12 of metal exposure [29].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Copper- and Zinc- Binding Metallothioneins from the Marine Alga Ulva compressa (Chlorophyta)

Zúñiga

Laporte

González

et al. 2019

IJMS

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In this work, transcripts encoding three metallothioneins from Ulva compressa (UcMTs) were amplified: The 5′and 3′ UTRs by RACE-PCR, and the open reading frames (ORFs) by PCR. Transcripts encoding UcMT1.1 (Crassostrea-like), UcMT2 (Mytilus-like), and UcMT3 (Dreissena-like) showed a 5′UTR of 61, 71, and 65 nucleotides and a 3′UTR of 418, 235, and 193 nucleotides, respectively. UcMT1.1 ORF encodes a protein of 81 amino acids (MW 8.2 KDa) with 25 cysteines (29.4%), arranged as three motifs CC and nine motifs CXC; UcMT2 ORF encode a protein of 90 amino acids (9.05 kDa) with 27 cysteines (30%), arranged as three motifs CC, nine motifs CXC, and one motif CXXC; UcMT3 encode a protein of 139 amino acids (13.4 kDa) with 34 cysteines (24%), arranged as seven motifs CC and seven motifs CXC. UcMT1 and UcMT2 were more similar among each other, showing 60% similarity in amino acids; UcMT3 showed only 31% similarity with UcMT1 and UcMT2. In addition, UcMTs displayed structural similarity with MTs of marine invertebrates MTs and the terrestrial invertebrate Caenorhabtidis elegans MTs, but not with MTs from red or brown macroalgae. The ORFs fused with GST were expressed in bacteria allowing copper accumulation, mainly in MT1 and MT2, and zinc, in the case of the three MTs. Thus, the three MTs allowed copper and zinc accumulation in vivo. UcMTs may play a role in copper and zinc accumulation in U. compressa.

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Gracilaria tenuistipitata (Rhodophyta) tolerance to cadmium and copper exposure observed through gene expression and photosynthesis analyses

et al. 2018

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Copper-induced overexpression of genes encoding antioxidant system enzymes and metallothioneins involve the activation of CaMs, CDPKs and MEK1/2 in the marine alga Ulva compressa

Cited by 34 publications

References 33 publications

Copper-induced concomitant increases in photosynthesis, respiration, and C, N and S assimilation revealed by transcriptomic analyses in Ulva compressa (Chlorophyta)

Copper-induced concomitant increases in photosynthesis, respiration, and C, N and S assimilation revealed by transcriptomic analyses in Ulva compressa (Chlorophyta)

Isolation and Characterization of Copper- and Zinc- Binding Metallothioneins from the Marine Alga Ulva compressa (Chlorophyta)

Gracilaria tenuistipitata (Rhodophyta) tolerance to cadmium and copper exposure observed through gene expression and photosynthesis analyses

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