Copper-induced oxidative stress, transcriptome changes, intestinal microbiota, and histopathology of common carp (Cyprinus carpio)

Zhang, Lixia; Yang, Zi; Yang, Mengxiao; Yang, Fan; Wang, Gege; Liu, Dandan; Li, Xuejun; Yang, Lianlian; Wang, Zhanqi

doi:10.1016/j.ecoenv.2022.114136

Cited by 31 publications

(8 citation statements)

References 98 publications

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“…Nevertheless, excessive oxidative stress can lead to tissue damage. Previous studies have demonstrated that oxidoreductase activity, iron ion binding, and copper ion transport are closely related to oxidative stress [33,34]. Thus, il-6 mutation may markedly affect the liver redox state during A. hydrophila infection.…”

Section: Discussionmentioning

confidence: 98%

IL-6 Mutation Attenuates Liver Injury Caused by Aeromonas hydrophila Infection by Reducing Oxidative Stress in Zebrafish

Zhai,

Wang,

et al. 2023

IJMS

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Interleukin-6 (IL-6), a pleiotropic cytokine, plays a crucial role in acute stress induced by bacterial infection and is strongly associated with reactive oxygen species (ROS) production. However, the role of IL-6 in the liver of fish after Aeromonas hydrophila infection remains unclear. Therefore, this study constructed a zebrafish (Danio rerio) il-6 knockout line by CRISPR/Cas9 to investigate the function of IL-6 in the liver post bacterial infection. After infection with A. hydrophila, pathological observation showed that il-6−/− zebrafish exhibited milder liver damage than wild-type (WT) zebrafish. Moreover, liver transcriptome sequencing revealed that 2432 genes were significantly up-regulated and 1706 genes were significantly down-regulated in il-6−/− fish compared with WT fish after A. hydrophila infection. Further, gene ontology (GO) analysis showed that differentially expressed genes (DEGs) were significantly enriched in redox-related terms, including oxidoreductase activity, copper ion transport, etc. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that DEGs were significantly enriched in pathways such as the PPAR signaling pathway, suggesting that il-6 mutation has a significant effect on redox processes in the liver after A. hydrophila infection. Additionally, il-6−/− zebrafish exhibited lower malondialdehyde (MDA) levels and higher superoxide dismutase (SOD) activities in the liver compared with WT zebrafish following A. hydrophila infection, indicating that IL-6 deficiency mitigates oxidative stress induced by A. hydrophila infection in the liver. These findings provide a basis for further studies on the role of IL-6 in regulating oxidative stress in response to bacterial infections.

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Section: Discussionmentioning

confidence: 98%

IL-6 Mutation Attenuates Liver Injury Caused by Aeromonas hydrophila Infection by Reducing Oxidative Stress in Zebrafish

Zhai,

Wang,

et al. 2023

IJMS

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“…In fish, glutathione peroxidase (GSH-PX) is a common antioxidant enzyme that can remove excess damaging reactive oxygen species (ROS) and protect cells from damage caused by ROS through lipid peroxidation [ [36] , [37] , [38] ]. Malondialdehyde (MDA) is used as a biomarker of lipid peroxidation in tissues, which can reflect the degree of oxidative damage in vivo, and high levels of MDA lead to high cytotoxicity [ 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Bacillus halophilus on growth, intestinal flora and metabolism of Larimichthys crocea

Huang,

Shui,

Wang

et al. 2023

Biochemistry and Biophysics Reports

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“…[ 79 , 80 ] Exposure to copper in mammals resulted in alterations in the composition, abundance, and diversity of the gut microbiota [ 81 , 82 ] and was accompanied by structural abnormalities in the intestinal epithelium, with marked vacuolization and enlargement of the lamina propria and nuclear shrinkage. [ 83 ]…”

Section: Mechanisms By Which Copper Overload Affects Cognitive Functionmentioning

confidence: 99%

Copper neurotoxicity: Induction of cognitive dysfunction: A review

Feng,

Zhao,

et al. 2023

Medicine

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Cognitive dysfunction occurs mainly in certain diseases and in the pathological process of aging. In addition to this, it is also widespread in patients undergoing anesthesia, surgery, and cancer chemotherapy. Neuroinflammation, oxidative stress, mitochondrial dysfunction, impaired synaptic plasticity, and lack of neurotrophic support are involved in copper-induced cognitive dysfunction. In addition, recent studies have found that copper mediates cuproptosis and adversely affects cognitive function. Cuproptosis is a copper-dependent, lipoylated mitochondrial protein-driven, non-apoptotic mode of regulated cell death, which provides us with new avenues for identifying and treating related diseases. However, the exact mechanism by which cuproptosis induces cognitive decline is still unclear, and this has attracted the interest of many researchers. In this paper, we analyzed the pathological mechanisms and therapeutic targets of copper-associated cognitive decline, mainly in the context of neurodegenerative diseases, psychiatric and psychological disorders, and diabetes mellitus.

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Copper-induced oxidative stress, transcriptome changes, intestinal microbiota, and histopathology of common carp (Cyprinus carpio)

Cited by 31 publications

References 98 publications

IL-6 Mutation Attenuates Liver Injury Caused by Aeromonas hydrophila Infection by Reducing Oxidative Stress in Zebrafish

IL-6 Mutation Attenuates Liver Injury Caused by Aeromonas hydrophila Infection by Reducing Oxidative Stress in Zebrafish

Effects of Bacillus halophilus on growth, intestinal flora and metabolism of Larimichthys crocea

Copper neurotoxicity: Induction of cognitive dysfunction: A review

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