Copper Pyrovanadate Nanoribbons as Efficient Multienzyme Mimicking Nanozyme for Biosensing Applications

Abstract

Development of nanozymes with intrinsic multienzyme mimetic activity has attracted tremendous research attention as nanozymes offer several advantages over multimodal natural enzymes and are a key toward multifunctional biomedical applications. Shape-selective ternary metal oxides with multicatalytic sites can be developed as potential multienzyme mimetic nanomaterials taking advantage of the variable valence states of the metal constituents and their synergy along with the added advantage offered by the exposed surface for enhanced catalytic activity. Herein, we have explored the multicatalytic activity of Cu 3 V 2 O 7 (OH) 2 •2H 2 O nanoribbons for peroxidase, oxidase, and laccase mimicking activity. The fabrication of Cu 3 V 2 O 7 (OH) 2 nanoribbons was performed using the polyol-based hydrothermal pathway, and the choice of precursor metal salts and their molar ratio was found to be instrumental in the shape, size, and phase selective evolution of copper pyrovanadates. The as-synthesized nanoribbons showed excellent peroxidase-like activity with K m value of 0.004 mM for H 2 O 2 , which is considerably smaller than those reported for HRP and other nanozymes. The efficient oxidase mimicking behavior of these Cu 3 V 2 O 7 (OH) 2 nanoribbons could be used for the colorimetric detection of glutathione with a limit of detection of 0.08 μM. Further, the nanoribbons also showed laccase-mimetic behavior and were used for the colorimetric detection of epinephrine, which is a useful hormone and neurotransmitter.

Robust and Reusable Laccase Mimetic Copper Oxide Nanozyme for Phenolic Oxidation and Biosensing

“…28−30 Therefore, a robust laccase mimetic nanozyme using cheaper materials would offer a great opportunity in realizing the practical applications otherwise inaccessible by the native enzyme. Different transition-metal oxides such as CeO 2 , γ-MnO 2 , 31,32 and Cu 3 V 2 O 7 (OH) 2 28 were demonstrated to exhibit laccase-like catalytic activity, catalyzing the oxidation of phenolic compounds in the absence of hydrogen peroxide. Fe 3 O 4 nanoparticle-templated materials including Fe 3 O 4 @ MnO x 33 and Fe 3 O 4 @Cu/GMP 18 provided magnetically separable laccase mimetics.…”

A galvanic replacement reaction and the Kirkendall effect in the room-temperature synthesis of tubular NiSe2: a nanozyme catalyst with peroxidase-like activity

“…Hence, different analytical techniques have been developed for the detection of GSH. 27–29 Here it is worth mentioning that the H 2 O 2 -mediated facile oxidation of TMB in the presence of the NiSe 2 nanozyme was found to be arrested by GSH in succession and, thus, becomes an intriguing platform for the colorimetric detection of this important biomolecule, GSH.…”

Current research progress on laccase-like nanomaterials

“…The active centers of natural laccase are copper ions. Recently, a number of copper-based nanomaterials, such as copper-nucleotide/DNA coordination compounds, 46–51 copper-amino acid/peptide/protein coordination compounds, 52–56 and other copper-based nanomaterials, 57–62 have been developed to simulate laccase activity. Besides copper-containing nanomaterials, other metal-based nanomaterials have been found to have laccase activity, for example, platinum nanoparticles, 63 manganese oxides, 64 etc.…”

“…The laccase-mimetic activity of this nanozyme could be applied to the assay of adrenaline. 58 Liu and co-workers obtained “raisin pudding”-type ZIF-67/Cu 0.76 Co 2.24 O 4 nanospheres with multi-enzyme activity by adjusting the synthesis ratio of the raw materials. A real-time online electrochemical assay of 3,4-dihydroxyphenylacetic acid was established based on the laccase-like activity of this nanozyme.…”