Copper removal ability by Streptomyces strains with dissimilar growth patterns and endowed with cupric reductase activity

Albarracín, Virginia Helena; Avila, Ana Lucía; Amoroso, Maria Julia del R.; Abate, Carlos Mauricio

doi:10.1016/j.jbiotec.2008.07.1639

Cited by 13 publications

(19 citation statements)

References 25 publications

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“…Moreover, a similar approach was used to determine cellular cupric reductase activity in Streptomyces sp. using the chelator bicinchoninic acid reagent (Albarracín et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Cu(II)-reduction by Escherichia coli cells is dependent on respiratory chain components

Volentini

Farı́as

Rodrı́guez-Montelongo

et al. 2011

Biometals

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Copper is both an essential nutrient and a toxic element able to catalyze free radicals formation which damage lipids and proteins. Although the available copper redox species in aerobic environment is Cu(II), proteins that participate in metal homeostasis use Cu(I). With isolated Escherichia coli membranes, we have previously shown that electron flow through the respiratory chain promotes cupric ions reduction by NADH dehydrogenase-2 and quinones. Here, we determined Cu(II)-reductase activity by whole cells using strains deficient in these respiratory chain components. Measurements were done by the appearance of Cu(I) in the supernatants of cells exposed to sub-lethal Cu(II) concentrations. In the absence of quinones, the Cu(II)-reduction rate decreased ~70% in respect to the wild-type strain, while this diminution was about 85% in a strain lacking both NDH-2 and quinones. The decrease was ~10% in the absence of only NDH-2. In addition, we observed that quinone deficient strains failed to grow in media containing either excess or deficiency of copper, as we have described for NDH-2 deficient mutants. Thus, the Cu(II)-reduction by E. coli intact cells is mainly due to quinones and to a lesser extent to NDH-2, in a quinone-independent way. To our knowledge, this is the first in vivo demonstration of the involvement of E. coli respiratory components in the Cu(II)-reductase activity which contributes to the metal homeostasis.

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“…Moreover, a similar approach was used to determine cellular cupric reductase activity in Streptomyces sp. using the chelator bicinchoninic acid reagent (Albarracín et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Cu(II)-reduction by Escherichia coli cells is dependent on respiratory chain components

Volentini

Farı́as

Rodrı́guez-Montelongo

et al. 2011

Biometals

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“…Intracellular localization of chromium was examined ultracytochemically using a modified procedure of Timm's reagent method for metal staining (Albarracín et al 2008a). Pellets of Streptomyces sp.…”

Section: Morphological Studies and Ultrastructural Determination Of Cmentioning

confidence: 99%

“…MC1 cells cultivated with and without Cr(VI) were processed without staining: fixation (glutaraldehyde 3% in phosphate buffer 0.1 M pH 7.4) for 3 h at 4°C followed by osmium tetroxide (1% in the same buffer). Samples were then dehydrated in an alcohol series transferred to acetone and embedded in Spurr resin (Albarracín et al 2008a) 2.4 Scanning Electron Microscopy and EDXA Bacteria cells were harvested with centrifugation (10,000 rpm, 4°C, 30 min). Cell pellets were washed with distilled water, fixed in Karnovsky's formaldehyde (8%), glutaraldehyde (16%), and phosphate buffer (pH 7) over night at 4°C, the fixed samples were washed three times with phosphate buffer and CaCl for 10 min.…”

Section: Morphological Studies and Ultrastructural Determination Of Cmentioning

confidence: 99%

“…Metal-resistant actinobacteria, and their potential use for bioremediation strategies, have been described before (Amoroso et al 1998;Albarracín et al 2005Albarracín et al , 2008aKothe et al 2005;Schmidt et al 2005;Polti et al 2007Polti et al , 2009). Among the soil filamentous microorganisms, streptomycetes represent up to 20% of soil bacteria .…”

Section: Introductionmentioning

confidence: 99%

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Intracellular chromium accumulation by Streptomyces sp. MC1

Polti

Amoroso

Abate

2010

Water Air Soil Pollut

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Streptomyces sp. MC1, previously isolated from sugar cane, has shown ability to reduce Cr(VI) in liquid minimal medium and soil samples. The objective of this work was to demonstrate the intracellular chromium accumulation by Streptomyces sp. MC1 under different culture conditions. This strain was able to accumulate up to 3.54 mg of Cr (III) per gram of wet biomass, reducing the 98% of Cr (VI) and removing 13.9% of chromium from the culture medium supernatants. Streptomyces sp. MC1 chromium bioaccumulation ability was corroborated by using Timm's reagent technique, a low-cost method, which has been used by first time to detect chromium deposits in bacteria. The results of atomic absorption spectrometry, scanning electron microscopy, and energy dispersive X-ray analysis suggest that the mechanism of Cr(VI) resistance observed in Streptomyces sp. MC1 includes adsorption coupled with reduction to Cr(III), and finally, Cr(III) bioaccumulation. This mechanism have special relevance to remediation of Cr(VI) contaminated environments by Streptomyces sp. MC1.

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“…They are of special interest for biotechnology and bioremediation because of their enormous metabolic versatility and several known metal resistance patterns (Albarracin et al, 2008;Benimeli et al, 2011;Haferburg and Kothe, 2010).…”

Section: Introductionmentioning

confidence: 99%

Live and death of streptomyces in soil—what happens to the biomass?

Schütze

Miltner

Nietzsche

et al. 2013

Z. Pflanzenernähr. Bodenk.

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The formation of soil organic matter (SOM) has been proposed to depend on fragmentation of biomass after cell death. However, this is hard to mimic in laboratory experiments showing the process directly. We used heavy metal contamination in order to provide an environment in which one Streptomyces strain, the heavy metal resistant S. mirabilis P16B-1, could survive while the sensitive strain S. lividans TK24 was expected to die and disintegrate; the necromass fragments would then contribute to SOM formation. Both strains were grown for 30 d in sterile mesocosms containing either highly metal-contaminated soil from a former uranium-mining site in Ronneburg, Germany, or control soil from a municipal park, Jena, Germany. The fate and morphology of living and dead bacterial biomass (necromass) was observed using scanning electron microscopy. Attachment of soil particles to the intact mycelium as well as decay of dead biomass was observed. Dead bacterial biomass was identified in form of patchy fragments while the superordinate filamentous structure of the hyphae was still visible and obviously stabilized in soil. The fate of cytosolic compounds was followed using the example of a nickel-containing superoxide dismutase (NiSOD) which was found to be released after death of cells grown in liquid soil-extract medium. Activity of the enzyme was proven for concentrated media supernatant by a gel-based qualitative activity assay. This indicates that NiSOD remains active in soil after cell death. Hence, bacterial cell death results in the release of cytosolic compounds, e.g., intact proteins, as well as the formation of residual cell-envelope fragments contributing to SOM formation.

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Copper removal ability by Streptomyces strains with dissimilar growth patterns and endowed with cupric reductase activity

Cited by 13 publications

References 25 publications

Cu(II)-reduction by Escherichia coli cells is dependent on respiratory chain components

Cu(II)-reduction by Escherichia coli cells is dependent on respiratory chain components

Intracellular chromium accumulation by Streptomyces sp. MC1

Live and death of streptomyces in soil—what happens to the biomass?

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