Copper resistance in Anabaena variabilis: Effects of phosphate nutrition and polyphosphate bodies

Hashemi, Forough Sadat; Leppard, Gary G.; Kushnert, D.J.

doi:10.1007/bf00165815

Cited by 41 publications

(24 citation statements)

References 34 publications

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“…47,48 Investigations with a strain of Cu-sensitive and Cu-tolerant freshwater cyanobacterium, Anabaena variabilis, indicated that the tolerant strain contained more polyphosphate bodies than the sensitive strain and, even more polyphosphate bodies were observed after exposure to Cu. 47 However, X-ray microanalyses showed that Cu did not co-localise with these polyphosphate bodies (identified after staining with toluidine blue and microscopy), with only P and Ca detected. 47 Instead, Cu was detected in the cytoplasm (co-located with P, S, Ca, Mg, Si and S).…”

Section: Discussionmentioning

confidence: 99%

Copper Uptake, Intracellular Localization, and Speciation in Marine Microalgae Measured by Synchrotron Radiation X-ray Fluorescence and Absorption Microspectroscopy

Adams

Dillon

Vogt

et al. 2016

Environ. Sci. Technol.

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Metal toxicity to aquatic organisms depends on the speciation of the metal and its binding to the critical receptor site(s) (biotic ligand) of the organism. The intracellular nature of the biotic ligand for Cu in microalgal cells was investigated using the high elemental sensitivity of microprobe synchrotron radiation X-ray fluorescence (SR-XRF) and X-ray absorption near-edge spectroscopy (XANES). The marine microalgae, Ceratoneis closterium, Phaeodactylum tricornutum, and Tetraselmis sp. were selected based on their varying sensitivities to Cu (72-h 50% population growth inhibitions of 8–47 μg Cu/L). Intracellular Cu in control cells was similar for all three species (2.5–3.2 × 10–15 g Cu/cell) and increased 4-fold in C. closterium and Tetraselmis sp. when exposed to copper, but was unchanged in P. tricornutum (72-h exposure to 19, 40, and 40 μg Cu/L, respectively). Whole cell microprobe SR-XRF identified endogenous Cu in the central compartment (cytoplasm) of control (unexposed) cells. After Cu exposure, Cu was colocated with organelles/granules dense in P, S, Ca, and Si and this was clearly evident in thin sections of Tetraselmis sp. XANES indicated coexistence of Cu(I) and Cu(II) in control and Cu-exposed cells, with the Cu ligand (e.g., phytochelatin) in P. tricornutum different from that in C. closterium and Tetraselmis sp. This study supports the hypothesis that Cu(II) is reduced to Cu(I) and that polyphosphate bodies and phytochelatins play a significant role in the internalization and detoxification of Cu in marine microalgae.

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Section: Discussionmentioning

confidence: 99%

Copper Uptake, Intracellular Localization, and Speciation in Marine Microalgae Measured by Synchrotron Radiation X-ray Fluorescence and Absorption Microspectroscopy

Adams

Dillon

Vogt

et al. 2016

Environ. Sci. Technol.

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“…Twiss and Nalewajko [46] observed that increasing the amount of cellular polyphosphate in UTEX 72 was very effective in reducing Cu toxicity to photosynthesis but was relatively insignificant in B4 and Cu-Tol. Polyphosphate bodies may have some effect on Cu resistance in Anabaena variabilis but are not completely responsible for this resistance [8].…”

Section: Discussionmentioning

confidence: 99%

Comparative study of nickel toxicity to growth and photosynthesis in nickel-resistant and -sensitive strains of Scenedesmus acutus f. alternans (Chlorophyceae)

1996

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Nickel (Ni) toxicity to growth and photosynthesis was studied in four strains of Scenedesmus acutus f. alternans. Effects of Ni dosage and duration of exposure on growth and photosynthesis were strain specific. Large differences in responses of both growth and photosynthesis to Ni were detected between three resistant strains (B4, Cu-Tol, and Ni-Tol) and one sensitive strain (UTEX 72). Growth of UTEX 72 was ≥ 18 times more sensitive to Ni than those of the three resistant strains. The order of Ni dosages (fmol Ni/pg cell dry weight) causing 50% inhibition (D150) of growth rates in the four strains was Ni-Tol (10.5) > B4 (8.19) > Cu-Tol (4.60) > UTEX 72 (0.25). The effect of Ni dosage on photosynthetic rate as percentage of control corresponded to a saturation curve and was a strong function of duration of exposure. The DI50s of photosynthetic rates were ≥3.5 times lower in UTEX 72 than in the three resistant strains, and in all four strains they decreased sharply with the increase in duration of exposure. The order of the four strains in DI50s of photosynthetic rate was B4 (58.2) > Cu-Tol (38.0) > Ni-Tol (28.9) > UTEX 72 (8.24) for 6-h exposure and Ni-Tol (2.88) > Cu-Tol (1.30) > B4 (1.01) > UTEX 72 (0.15) for 24-h exposure. The DI50s of photosynthetic rate for 6-h exposure were higher than those of growth rate in all four strains, and for 24-h exposure they were lower, except in UTEX 72. Thus, the relative Ni sensitivity of growth and photosynthesis of the four strains depends on the duration of exposure. The results of factorial analysis of variance suggested that Ni toxicity to photosynthesis is a consequence of a strong interaction among strain, Ni dosage, and duration of exposure.

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“…Acidocalcisomal poly P stores are important for resistance to heavy metals in some eukaryotes and prokaryotes (Hashemi et al 1994;Alvarez & Jerez 2004;Andrade et al 2004;Remonsellez et al 2006;Nagasaka & Yoshimura 2008). Thus, phosphate and heavy metal storage may represent an ancestral characteristic that was important for their evolutionary development.…”

Section: Introductionmentioning

confidence: 99%

Evolution of acidocalcisomes and their role in polyphosphate storage and osmoregulation in eukaryotic microbes

Docampo

Ulrich

Moreno

2010

Phil. Trans. R. Soc. B

104

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Acidocalcisomes are acidic electron-dense organelles, rich in polyphosphate (poly P) complexed with calcium and other cations. While its matrix contains enzymes related to poly P metabolism, the membrane of the acidocalcisomes has a number of pumps (Ca 2+ -ATPase, V-H + -ATPase, H + -PPase), exchangers (Na + /H + , Ca 2+ /H + ), and at least one channel (aquaporin). Acidocalcisomes are present in both prokaryotes and eukaryotes and are an important storage of cations and phosphorus. They also play an important role in osmoregulation and interact with the contractile vacuole complex in a number of eukaryotic microbes. Acidocalcisomes resemble lysosome-related organelles (LRO) from mammalian cells in many of their properties. They share similar morphological characteristics, acidic properties, phosphorus contents and a system for targeting of their membrane proteins through adaptor complex-3 (AP-3). Storage of phosphate and cations may represent the ancestral physiological function of acidocalcisomes, with cation and pH homeostasis and osmoregulatory functions derived following the divergence of prokaryotes and eukaryotes.

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Copper resistance in Anabaena variabilis: Effects of phosphate nutrition and polyphosphate bodies

Cited by 41 publications

References 34 publications

Copper Uptake, Intracellular Localization, and Speciation in Marine Microalgae Measured by Synchrotron Radiation X-ray Fluorescence and Absorption Microspectroscopy

Copper Uptake, Intracellular Localization, and Speciation in Marine Microalgae Measured by Synchrotron Radiation X-ray Fluorescence and Absorption Microspectroscopy

Comparative study of nickel toxicity to growth and photosynthesis in nickel-resistant and -sensitive strains of Scenedesmus acutus f. alternans (Chlorophyceae)

Evolution of acidocalcisomes and their role in polyphosphate storage and osmoregulation in eukaryotic microbes

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