Copper toxicity leads to accumulation of free amino acids and polyphenols in Phaeodactylum tricornutum diatoms

Santiago-Díaz, Paula; Rivero, Argimiro; Rico, Milagros; González, Aridane G.; González‐Dávila, Melchor; Santana-Casiano, J. Magdalena

doi:10.1007/s11356-023-25939-0

Cited by 10 publications

(16 citation statements)

References 67 publications

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“…High concentrations of metals such as copper induce stress in phytoplankton that changes their growth patterns as well as their interaction with the environment. Figure 1 summarizes the effects of Cu toxicity on P. tricornutum diatom growth, previously reported by Santiago-Díaz et al 25 In brief, a reduction in both the growth curve and the biomass generated was observed. The mean absolute growth rate was 2.11 × 10 7 cell L −1 day −1 achieving a maximum cell density of 33.6 × 10 7 cell L −1 during the stationary phase, which ended after 16 days.…”

Section: Resultsmentioning

confidence: 52%

“…A lack of an exponential phase of growth and cell density decreases of 82 and 91% were observed at the highest Cu concentrations (0.79 and 1.57 µM, respectively), indicating that these Cu levels were lethal for P. tricornutum under our experimental conditions. A more detailed description is given by Santiago-Díaz et al 25 .…”

Section: Resultsmentioning

confidence: 99%

“…Santiago-Díaz et al 25 reported the accumulation of intracellular free amino acids and polyphenols after 18 days of exposure to lethal levels of Cu (Table 1 ). Under these conditions, the total sum of all polyphenols identified in the cells increased up to 11.3 times compared to the control (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The aim of this work was to evaluate the content of polyphenols and carbohydrates released by the diatom P. tricornutum grown for 12 and 18 days in natural seawater as a control, and in seawater enriched with three copper concentrations lower than those found in polluted coastal areas 13 , 15 , two lethal and one sublethal (1.57, 0.79 and 0.31 µM, respectively). The effects of Cu toxicity on the growth and intracellular productivity of these diatoms have been previously reported 25 . The present study is focused on the change in the characterization of both polyphenols and carbohydrates exuded by the diatoms grown under the same conditions in cultures, where the only variable modified was the copper concentration.…”

Section: Introductionmentioning

confidence: 97%

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Characterization of polyphenols and carbohydrates exuded by Phaeodactylum tricornutum diatom grown under Cu stress

Rico,

Santiago-Díaz,

Rivero

et al. 2024

Sci Rep

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This study is focused on analysing polyphenols and carbohydrates released by Phaeodactylum tricornutum (P. tricornutum) diatoms cultured in natural seawater enriched with sublethal and lethal Cu doses. Cu concentrations of 0.31, 0.79 and 1.57 µM reduced cell densities by 37, 82 and 91%, respectively, compared to the control. The total sum of all identified polyphenols and total carbohydrates released by cells grown under lethal Cu levels increased up to 18.8 and 107.4 times, respectively, compared to data from a control experiment. Four different in vitro assays were used to estimate the antioxidant activities of the extracellular compounds: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical inhibition, cupric ion reducing antioxidant capacity (CUPRAC), ferric reducing antioxidant power and Cu complexing ability (CCA). The highest antioxidant activities were observed in the Cu lethal treatments, where the CCA assay exhibited a greater increase (up to 32.2 times higher than that found in the control experiment) to reduce the concentration of free Cu in the medium and its toxicity. The presence of Cu stimulated the release of polyphenols and carbohydrates to the medium as a detoxification mechanism to survive under lethal levels of Cu regulating its speciation.

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Section: Resultsmentioning

confidence: 52%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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Characterization of polyphenols and carbohydrates exuded by Phaeodactylum tricornutum diatom grown under Cu stress

Rico,

Santiago-Díaz,

Rivero

et al. 2024

Sci Rep

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“…Diatoms in general are rich sources of polyphenolic compounds exhibiting antioxidant properties [56]. Studies show that diatom Phaeodactylum tricornutum under copper stress, releases high concentrations of polyphenols such as 4-hydroxycinnamic acid and chlorogenic acid [57, 58].…”

Section: Discussionmentioning

confidence: 99%

Untargeted Metabolomics to Expand the Chemical Space of the Marine DiatomSkeletonema marinoi

Zulfiqar,

Stettin,

Schmidt

et al. 2023

Preprint

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Diatoms (Bacillariophyceae) are aquatic photosynthetic microalgae with an ecological role as primary producers in the aquatic food web. They account substantially for global carbon, nitrogen, and silicon cycling. Elucidating the chemical space of diatoms is crucial to understanding their physiology and ecology. To expand the known chemical space of a cosmopolitan marine diatom,Skeletonema marinoi, we performed High-Resolution Liquid Chromatography-Tandem Mass Spectrometry (LC-MS2) for untargeted metabolomics data acquisition. The spectral data from LC-MS2was used as input for the Metabolome Annotation Workflow (MAW) to obtain putative annotations for all measured features. A suspect list of metabolites previously identified in theSkeletonemaspp. was generated to verify the results. These known metabolites were then added to the putative candidate list from LC-MS2data to represent an expanded catalogue of 1970 metabolites estimated to be produced byS. marinoi. The most prevalent chemical superclasses, based on the ChemONT ontology in this expanded dataset, were “Organic acids and derivatives”, “Organoheterocyclic compounds”, “Lipids and lipid-like molecules”, and “Organic oxygen compounds”. The metabolic profile from this study can aid the bioprospecting of marine microalgae for medicine, biofuel production, agriculture, and environmental conservation. The proposed analysis can be applicable for assessing the chemical space of other microalgae, which can also provide molecular insights into the interaction between marine organisms and their role in the functioning of ecosystems.ImportanceDiatoms are abundant marine phytoplankton members and have great ecological importance and biochemical potential. The cosmopolitan diatomSkeletonema marinoihas become an ecological and environmental research model organism. In this study, we used untargeted metabolomics to acquire a general metabolic profile ofS. marinoito assess its chemical diversity and expand the known metabolites produced by this diatom.S. marinoiproduces a chemically diverse set of secondary metabolites with potential therapeutic properties, such as anti-cancer, antioxidant, and anti-inflammatory. Such metabolites are highly significant due to their potential role in drug discovery and bioeconomy. Lipids fromS. marinoialso have potential in the biofuel industry. Furthermore, the environmental fluctuations in the water bodies directly affect the production of different secondary metabolites from diatoms, which can be key indicators of climate change.

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Application of high throughput in vitro metabolomics for hepatotoxicity mode of action characterization and mechanistic-anchored point of departure derivation: a case study with nitrofurantoin

Ramirez-Hincapie,

Birk,

Ternes

et al. 2023

Arch Toxicol

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Omics techniques have been increasingly recognized as promising tools for Next Generation Risk Assessment. Targeted metabolomics offer the advantage of providing readily interpretable mechanistic information about perturbed biological pathways. In this study, a high-throughput LC–MS/MS-based broad targeted metabolomics system was applied to study nitrofurantoin metabolic dynamics over time and concentration and to provide a mechanistic-anchored approach for point of departure (PoD) derivation. Upon nitrofurantoin exposure at five concentrations (7.5 µM, 15 µM, 20 µM, 30 µM and 120 µM) and four time points (3, 6, 24 and 48 h), the intracellular metabolome of HepG2 cells was evaluated. In total, 256 uniquely identified metabolites were measured, annotated, and allocated in 13 different metabolite classes. Principal component analysis (PCA) and univariate statistical analysis showed clear metabolome-based time and concentration effects. Mechanistic information evidenced the differential activation of cellular pathways indicative of early adaptive and hepatotoxic response. At low concentrations, effects were seen mainly in the energy and lipid metabolism, in the mid concentration range, the activation of the antioxidant cellular response was evidenced by increased levels of glutathione (GSH) and metabolites from the de novo GSH synthesis pathway. At the highest concentrations, the depletion of GSH, together with alternations reflective of mitochondrial impairments, were indicative of a hepatotoxic response. Finally, a metabolomics-based PoD was derived by multivariate PCA using the whole set of measured metabolites. This approach allows using the entire dataset and derive PoD that can be mechanistically anchored to established key events. Our results show the suitability of high throughput targeted metabolomics to investigate mechanisms of hepatoxicity and derive point of departures that can be linked to existing adverse outcome pathways and contribute to the development of new ones.

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Copper toxicity leads to accumulation of free amino acids and polyphenols in Phaeodactylum tricornutum diatoms

Cited by 10 publications

References 67 publications

Characterization of polyphenols and carbohydrates exuded by Phaeodactylum tricornutum diatom grown under Cu stress

Characterization of polyphenols and carbohydrates exuded by Phaeodactylum tricornutum diatom grown under Cu stress

Untargeted Metabolomics to Expand the Chemical Space of the Marine DiatomSkeletonema marinoi

Application of high throughput in vitro metabolomics for hepatotoxicity mode of action characterization and mechanistic-anchored point of departure derivation: a case study with nitrofurantoin

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