Copper, zinc superoxide dismutase from Caragana jubata : A thermostable enzyme that functions under a broad pH and temperature window

Kumar, Arun; Sharma, Meetal; Bhardwaj, Pardeep Kumar; Vats, Surender Kumar; Singh, Dharam; Kumar, Sanjay

doi:10.1016/j.procbio.2016.06.025

Cited by 10 publications

(5 citation statements)

References 50 publications

(65 reference statements)

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“…SOD activity was measured in units per mg of protein. One unit of the activity of an enzyme is the amount of enzyme required to suppress NBT reduction by 50% at room temperature in 10 min as per the following formula: Percent inhibition = [( A 560 control − A 560 sample)/ A 560 control] × 100 ( Kumar et al., 2016 ).…”

Section: Methodsmentioning

confidence: 99%

Synergistic effect of graphene oxide and silver nanoparticles as biostimulant improves the postharvest life of cut flower bird of paradise (Strelitzia reginae L.)

et al. 2022

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The bird of paradise (Strelitzia reginae L.) is one of the important tropical cut flowers. Generally, flowers like bird of paradise (BOP) grown for the commercial ornamental market must be of high pre and postharvest quality. Thus, to improve the postharvest longevity and increase marketability, the relative efficacy of two different biologically synthesized nanoparticles (NPs) was evaluated. The novel proprietary stimulants were graphene oxide (GO) and silver nanoparticles (SNPs). The NP treatments were applied as a vase (lower concentrations) solutions. Among all the applied treatments, the synergistic effect of GO + SNPs at 1 µL L−1 vase solution significantly (p =0.05) prolongs the post-harvest life of cut flowers of BOP. Increased vase life over the deionized water (DI) control was associated with better maintenance of relative water uptake, relative fresh weight, suppressed microbial density at stem-end and delay of stem blockage, reduced electrolyte leakage, malondialdehyde (MDA), SOD, and POD activity. In contrast to control, administration of NPs gave better results for all analyzed parameters. Application of biologically synthesized NPs in combination (GO + SNPs at 1 µL L−1) extended the vase life of cut flowers by 6 days compared with control flowers, and overall, showed better results than the control. The findings of the studies revealed that the standardized NPs could have more potential in prolonging the postharvest life of cut flowers in BOP. Thus, this technique can be used as a novel postharvest technology for commercial application in cut flowers.

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Section: Methodsmentioning

confidence: 99%

Synergistic effect of graphene oxide and silver nanoparticles as biostimulant improves the postharvest life of cut flower bird of paradise (Strelitzia reginae L.)

et al. 2022

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“…limon (AF318938, Lin et al, 2002 ), C . jubata (EF530044, Kumar et al, 2016b ), and A . marina (ACA50531.1, Fesharaki-Esfahani et al, 2021 ) by the CFSSP online tool.…”

Section: Resultsmentioning

confidence: 99%

“…However, OsCSD4 displayed higher specific activity, and the sequence variations seem associated with its stability at higher pH and temperature. The effects on characteristics due to non-critical amino acid changes might be associated with the observed local structural variations in SODs ( Lin et al, 2002 ; Kumar et al, 2012 , 2014 , 2016b ; Mahanty et al, 2012 ; Mishra et al, 2014 ; Tuteja et al, 2015 ; Sanyal et al, 2018 ; Fesharaki-Esfahani et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…Eukaryotic CSDs, with high β-sheet and low α-helical content, exhibit conservation of structurally/functionally important sites ( Khanna-Chopra and Sabarinath, 2004 ). However, variations in non-critical regions often affect important characteristics (e.g., specific activity, stability, subunit interaction, pH range), as reported in certain plant CSDs ( Lin et al, 2002 ; Kumar et al, 2012 , 2014 , 2016b ; Mahanty et al, 2012 ; Mishra et al, 2014 ; Tuteja et al, 2015 ; Sanyal et al, 2018 ; Fesharaki-Esfahani et al, 2021 ). The response of plant CSDs to different conditions have been extensively studied ( Gupta et al, 1993 ; Gill et al, 2015 ; Negi et al, 2015 ; Guan et al, 2017 ; Lightfoot et al, 2017 ; Del Río et al, 2018 ; Sanyal et al, 2018 ; Tyagi et al, 2019 ), where few other candidates/mechanisms are also involved in modulation of their expression/function, viz.…”

Section: Introductionmentioning

confidence: 99%

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Molecular and Biochemical Analysis of Duplicated Cytosolic CuZn Superoxide Dismutases of Rice and in silico Analysis in Plants

et al. 2022

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Superoxide dismutases (SODs, EC 1.15.1.1) are ubiquitous antioxidant metalloenzymes important for oxidative stress tolerance and cellular redox environment. Multiple factors have contributed toward the origin and diversity of SOD isoforms among different organisms. In plants, the genome duplication events, responsible for the generation of multiple gene copies/gene families, have also contributed toward the SOD diversity. However, the importance of such molecular events on the characteristics of SODs has not been studied well. This study investigated the effects of divergence on important characteristics of two block-duplicated rice cytosolic CuZn SODs (OsCSD1, OsCSD4), along with in silico assessment of similar events in other plants. The analysis revealed heterogeneity in gene length, regulatory regions, untranslated regions (UTRs), and coding regions of two OsCSDs. An inconsistency in the database-predicted OsCSD1 gene structure was also identified and validated experimentally. Transcript analysis showed differences in the basal levels and stress responsiveness of OsCSD1 and OsCSD4, and indicated the presence of two transcription start sites in the OsCSD1. At the amino acid level, the two OsCSDs showed differences at 18 sites; however, both exist as a homodimer, displaying typical CuZn SOD characteristics, and enhancing the oxidative stress tolerance of Escherichia coli cells. However, OsCSD4 showed higher specific activity as well as stability. The comparison of the two OsCSDs with reported thermostable CSDs from other plants identified regions likely to be associated with stability, while the homology modeling and superposition highlighted structural differences. The two OsCSDs displayed heteromeric interaction capability and forms an enzymatically active heterodimer (OsCSD1:OsCSD4) on co-expression, which may have significance as both are cytosolic. In silico analysis of 74 plant genomes revealed the prevalence of block duplications for multiple CSD copies (mostly cytosolic). The divergence and clustering analysis of CSDs suggested the possibility of an ancestral duplication event in monocots. Conserved SOD features indicating retention of SOD function among CSD duplicates were evident in few monocots and dicots. In most other species, the CSD copies lacked critical features and may not harbor SOD function; however, other feature-associated functions or novel functions might be present. These aspects of divergent CSD copies encoding co-localized CSDs may have implications in plant SOD functions in the cytosol and other organelles.

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“…While the zinc ion was found to be responsible for stabilizing the SOD dimer, the copper ion, via an alternate oxidation-reduction mechanism, helps this enzyme to catalyze a two-step superoxide dismutation reaction 6 . Certain Cu, Zn-SODs were also found to exhibit unaltered catalytic activity even in the presence of ionic detergents, chaotropic agents, extreme pH, and high temperature 7 – 9 . Because of its cellular abundance, diverse organellar distribution, high kinetic stability, and oxidative stress tolerance property Cu, Zn-SOD has become a good candidate for biochemical studies and subsequent biotechnological applications.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive characterization and molecular insights into the salt tolerance of a Cu, Zn-superoxide dismutase from an Indian Mangrove, Avicennia marina

Sarkar

Bhowmik

Sarkar

et al. 2022

Sci Rep

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Superoxide dismutases are important group of antioxidant metallozyme and play important role in ROS homeostasis in salinity stress. The present study reports the biochemical properties of a salt-tolerant Cu, Zn-superoxide from Avicennia marina (Am_SOD). Am_SOD was purified from the leaf and identified by mass-spectrometry. Recombinant Am_SOD cDNA was bacterially expressed as a homodimeric protein. Enzyme kinetics revealed a high substrate affinity and specific activity of Am_SOD as compared to many earlier reported SODs. An electronic transition in 360–400 nm spectra of Am_SOD is indicative of Cu2+-binding. Am_SOD activity was potentially inhibited by diethyldithiocarbamate and H2O2, a characteristic of Cu, Zn-SOD. Am_SOD exhibited conformational and functional stability at high NaCl concentration as well in alkaline pH. Introgression of Am_SOD in E. coli conferred tolerance to oxidative stress under highly saline condition. Am_SOD was moderately thermostable and retained functional activity at ~ 60 °C. In-silico analyses revealed 5 solvent-accessible N-terminal residues of Am_SOD that were less hydrophobic than those at similar positions of non-halophilic SODs. Substituting these 5 residues with non-halophilic counterparts resulted in > 50% reduction in salt-tolerance of Am_SOD. This indicates a cumulative role of these residues in maintaining low surface hydrophobicity of Am_SOD and consequently high salt tolerance. The molecular information on antioxidant activity and salt-tolerance of Am_SOD may have potential application in biotechnology research. To our knowledge, this is the first report on salt-tolerant SOD from mangrove.

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Copper, zinc superoxide dismutase from Caragana jubata : A thermostable enzyme that functions under a broad pH and temperature window

Cited by 10 publications

References 50 publications

Synergistic effect of graphene oxide and silver nanoparticles as biostimulant improves the postharvest life of cut flower bird of paradise (Strelitzia reginae L.)

Synergistic effect of graphene oxide and silver nanoparticles as biostimulant improves the postharvest life of cut flower bird of paradise (Strelitzia reginae L.)

Molecular and Biochemical Analysis of Duplicated Cytosolic CuZn Superoxide Dismutases of Rice and in silico Analysis in Plants

Comprehensive characterization and molecular insights into the salt tolerance of a Cu, Zn-superoxide dismutase from an Indian Mangrove, Avicennia marina

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