Copper/Zinc Superoxide Dismutases in Dictyostelium discoideum : Amino Acid Sequences and Expression Kinetics

Tsuji, Atsushi B.; Akaza, Yuya; Kodaira, Ken-Ichi; Yasukawa, Hiroo

doi:10.1080/10258140290018711

Cited by 5 publications

(3 citation statements)

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“…They are expressed during both mitotic growth and multicellular development. One study showed that sodA was induced by H2O2 but not UV, while sodB was induced by both H2O2 and UV (Tsuji et al, 2002). In contrast another study showed that sodA was not induced by either H2O2 or UV (Garcia et al, 2000).…”

Section: Antioxidant Defense Mechanisms In D Discoideummentioning

confidence: 99%

“…SOD in D. discoideum. D. discoideum encodes and expresses a number of SODs (Akaza et al, 2002;Garcia et al, 2000;Tsuji et al, 2002) corresponding to the SODs found in humans (Table 1.1).…”

Section: Antioxidant Defense Mechanisms In D Discoideummentioning

confidence: 99%

“…The sequences for the sodA, sodB, (Tsuji et al, 2002) and sodC (Tsuji et al, 2003) genes suggest that they are Cu/Zn enzymes. The sodA and sodB proteins both contain charged amino acids and hydrophobic stretches in the N-termini suggesting that they are extracellular.…”

Section: Antioxidant Defense Mechanisms In D Discoideummentioning

confidence: 99%

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Curcumin regulates transcription in Dictyostelium discoideum

Swatson¹,

William²

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Botanicals are widely used as dietary supplements and for the prevention and treatment of a myriad of diseases ranging from the common cold to depression. One in four Americans uses a botanical as part of their primary healthcare with billions of dollars spent annually. Curcumin, the active ingredient in turmeric, has gained a lot attention in recent years for its therapeutic uses and has been linked to a wide spectrum of pharmacological effects including anti-carcinogenic, anti-inflammatory, Alzheimer's prevention and antioxidant activity. Despite the large and growing interest in the use of botanicals such as curcumin in disease treatment and prevention, there is little evidence regarding their efficacy, safety and long-term effects. Importantly, the fundamental mechanisms associated with the cellular response to botanicals are generally not clearly understood, and there are often unknown off-target effects. This is supported by the bafflingly large number of effects associated with curcumin. The use of suitable model systems in pharmacogenetic analysis allows for the subsequent characterization of genes and their protein products to explain the mechanisms of drug action. Understanding the complex mechanisms associated with drug response is compounded by the use of mammalian models. In addition, the maintenance and care of such models, the large numbers of animals required for experimental study as well as ethical concerns have necessitated the use of simple and genetically tractable non-mammalian models. The social amoeba, Dictyostelium discoideum, has proven to be an excellent model for the molecular and genetic study of the mechanisms of action of drugs and their effects on the cell. D discoideum has been successfully used to find targets to improve efficacy of drugs used in psychiatry and cancer treatment. Those studies were subsequently validated in human cells. By taking advantage of this simple but powerful biological model and the molecular genetic tools available to us, we have started to investigate the complex effects of the botanical compound curcumin on cell growth, cell physiology and the underlying molecular mechanisms of action. Results from our studies revealed a rather complex pleiotropic response to curcumin including effects on proliferation, oxidative stress and a global regulation of gene expression at the transcriptional level. The relative lack of conclusive scientific evidence regarding the therapeutic benefits of curcumin, coupled with curcumin's possible deleterious effects as revealed by our research findings, underscore the importance of further research to establish curcumin's risks and benefits. Ascertaining the risk-benefits of curcumin with more conclusive scientific evidence would better equip consumers to make decisions about using curcumin, and in fact other botanicals, as part of their primary healthcare.

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Section: Antioxidant Defense Mechanisms In D Discoideummentioning

confidence: 99%

“…SOD in D. discoideum. D. discoideum encodes and expresses a number of SODs (Akaza et al, 2002;Garcia et al, 2000;Tsuji et al, 2002) corresponding to the SODs found in humans (Table 1.1).…”

Section: Antioxidant Defense Mechanisms In D Discoideummentioning

confidence: 99%

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Curcumin regulates transcription in Dictyostelium discoideum

Swatson¹,

William²

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Methylglyoxal upregulates Dictyostelium discoideum slug migration by triggering glutathione reductase and methylglyoxal reductase activity

Lee

Seo

Kwak

et al. 2017

The International Journal of Biochemistry & Cell Biology

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Molecular characterization of two superoxide dismutases from Hydra vulgaris

Dash

Metz

Huebner

et al. 2007

Gene

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Apparent full-length cDNA sequences coding for manganese superoxide dismutase (HvMnSOD) and extracellular superoxide dismutase (HvEC-SOD) were isolated from Hydra vulgaris in order to understand their expression and 3D structures; and explore their possibility of being used as for biomarkers for environmental stress and toxicity. The deduced HvMnSOD protein consists of 219 amino acids of which first 21 amino acids constitute a presumed mitochondria-targeting signal peptide whereas HvEC-SOD protein consists of 189 amino acids of which first 19 amino acids constitute a presumed signal peptide. Molecular model generated for HvMnSOD displayed the N-terminal long alpha antiparallel hairpin and the C-terminal mixed alpha/beta fold characteristic of MnSODs and that for HvEC-SOD displayed the characteristic CuZnSOD â-barrel fold. Hydrae subjected to thermal, starvation, metal and oxidative stress responded by regulating MnSOD and EC-SOD mRNA transcription. These results indicated that these genes are involved in the cellular stress response and (anti)oxidative processes triggered by stressor and contaminant exposure. Hence the expression of these SODs in hydra may have potential as molecular biomarkers for assessing stress, toxicity and pro-oxidant quality of chemicals and aquatic environmental quality.

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Copper/Zinc Superoxide Dismutases in Dictyostelium discoideum : Amino Acid Sequences and Expression Kinetics

Cited by 5 publications

References 0 publications

Curcumin regulates transcription in Dictyostelium discoideum

Curcumin regulates transcription in Dictyostelium discoideum

Methylglyoxal upregulates Dictyostelium discoideum slug migration by triggering glutathione reductase and methylglyoxal reductase activity

Molecular characterization of two superoxide dismutases from Hydra vulgaris

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