Coprecipitation of cytochrome C with calcium phosphate on hydroxyapatite ceramic

Sogo, Yu; Ito, Atsuo; Fukasawa, Koshiro; Kondo, Nao; Ishikawa, Yusuke; Ichinose, Noboru; Yamazaki, Atsushi

doi:10.1016/j.cap.2005.01.025

Cited by 32 publications

(26 citation statements)

References 15 publications

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“…19 Using the same technique, we coprecipitated cytochrome C with calcium phosphate on a hydroxyapatite ceramic, and cytochrome C was released from the ceramic for 10 days. 20 Cytochrome C has a molecular weight and an isoelectric point nearly equivalent to those of fibroblast growth factor-2 (FGF-2). FGF-2 accelerates wound healing and has clinical applications.…”

Section: Introductionmentioning

confidence: 99%

Fibroblast growth factor‐2‐apatite composite layers on titanium screw to reduce pin tract infection rate

et al. 2008

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Fibroblast growth factor-2 (FGF-2)-apatite composite layers were formed on anodically oxidized titanium screws to improve bone-screw interface strength and to reduce pin tract infection rate through enhanced skin tissue healing in external fixation. A calcium-containing solution supplemented with FGF-2, a phosphate-containing solution, and a sodium bicarbonate solution were mixed at a Ca/P molar ratio of 2.0 to prepare a calcium phosphate solution supersaturated with respect to calcium phosphates. Screws were individually immersed in 10 mL of the calcium phosphate solution at 37 degrees C for 2 days. Low-crystalline apatite layers incorporating FGF-2 were formed on the screw surface at FGF-2 concentrations in the supersaturated calcium phosphate solution equal to or lower than 10 mug/mL. The amounts of FGF-2 immobilized on the screws ranged from 2.3- to 2.4-mug per screw. The immobilized FGF-2 retained biological activity, as demonstrated by NIH3T3 cell proliferation. Titanium screws with the composite layer were percutaneously implanted into the bilateral proximal tibial metaphyses in rabbits for 4 weeks. The titanium screws with the composite layer formed at the optimum FGF-2 concentration showed a significantly higher bone-screw interface strength and a lower pin tract infection rate than those without the composite layer: the extraction torque and infection rates were respectively 0.230 +/- 0.073 Nm and 43.8% for the screws with the composite layer, and 0.170 +/- 0.056 Nm and 93.8% for those without the composite layer. Therefore, titanium screws with the FGF-2-apatite composite layer are useful for improving bone-screw interface strength and infection resistance in external skeletal fixation.

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Section: Introductionmentioning

confidence: 99%

Fibroblast growth factor‐2‐apatite composite layers on titanium screw to reduce pin tract infection rate

et al. 2008

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“…Recently, soaking the implants in supersaturated calcium phosphate solutions containing some biologically active molecules at physiological temperatures has been developed to form the coprecipitate of active molecules and apatite on the surface of the implants [11][12][13][14]. When implanted, these loaded molecules will release in the body and be of great value for the intergradation of the implants with the host tissue.…”

Section: Discussionmentioning

confidence: 98%

“…Sogo et al [14] has incorporated cyt C, in the homogeneously bone-like apatite layer by immersing hydroxyapatite disk in supersaturated calcium phosphates solution containing cyt C.…”

Section: Introductionmentioning

confidence: 99%

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Incorporation of cytochrome C with thin calcium phosphate film formed by electron-beam evaporation

Lee

Cui

et al. 2008

Surface and Coatings Technology

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“…The ability to hold and release various kinds of proteins, including FGF-2, has been studied [10,11]. In studies using cytochrome C (cyt C) as a harmless dummy protein, cyt C was effectively coated to a hydroxyapatite (HAP) ceramic, forming a cyt C/calcium phosphate composite layer in supersaturated calcium phosphate solutions prepared by mixing several types of infusion fluids [10].…”

Section: Introductionmentioning

confidence: 99%

Enhanced bone formation using hydroxyapatite ceramic coated with fibroblast growth factor-2

et al. 2010

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Our objective was to develop a bone substitute coated with fibroblast growth factor-2 (FGF-2) that subsequently releases FGF-2. We investigated the use of our system of bone substitutes to induce bone formation. Hydroxyapatite ceramic buttons (HAP-CBs) were coated with FGF-2 by precipitation in supersaturated calcium phosphate solution. HAP-CBs were coated with high or low doses of FGF-2, denoted as FGF-H and FGF-L. The release of FGF-2 from FGF-H and FGF-L was evaluated using its release profile and bioactivity. The efficacy of the subsequent bone formation was quantified using rats with round-shaped bone defects (5mm in diameter) of the right parietal bone. Group 1 was treated only with HAP-CBs, group 2 with HAP-CBs and drops of FGF-2 solution, group 3 with FGF-L and group 4 with FGF-H. To detect the release of FGF-2 in vivo, the expression of bone morphogenic protein-2 (BMP-2) was measured in the defective bone tissue. FGF-2 was released in vitro from FGF-H and FGF-L, and maintained its bioactivity. Rats treated with FGF-L showed better bone formation than rats from the other groups. BMP-2 expression was detected in the defective bone tissues of group 3 at 14 days, which might indicate in vivo FGF-2 release during this period. A specific FGF-2 concentration may be needed for bone formation, and our system can release FGF-2 at adequate concentrations to induce bone formation.

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Coprecipitation of cytochrome C with calcium phosphate on hydroxyapatite ceramic

Cited by 32 publications

References 15 publications

Fibroblast growth factor‐2‐apatite composite layers on titanium screw to reduce pin tract infection rate

Fibroblast growth factor‐2‐apatite composite layers on titanium screw to reduce pin tract infection rate

Incorporation of cytochrome C with thin calcium phosphate film formed by electron-beam evaporation

Enhanced bone formation using hydroxyapatite ceramic coated with fibroblast growth factor-2

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