Copro-PCR prevalence of <i>Echinococcus granulosus</i> infection in dogs in Kerman, south-eastern Iran

Mirbadie, Seyed Reza; Kamyabi, Hossein; Mohammadi, Mohsen; Shamsaddini, Saeedeh; Harandi, Majid Fasihi

doi:10.1017/s0022149x17000074

Cited by 11 publications

(12 citation statements)

References 22 publications

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“…It has been reported that there is no cross-amplification with Taenia hydatigena , T. ovis , Dipylidium caninum , or E. multilocularis (1). In previous studies from around the world, the prevalence of E. granulosus was found to be between 3.6% and 25.9% by molecular biological techniques (21). The present study using the copro-PCR technique revealed that E. granulosus infection is present in 4% of dog faecal samples in Van Province.…”

Section: Discussionmentioning

confidence: 96%

“…The present study using the copro-PCR technique revealed that E. granulosus infection is present in 4% of dog faecal samples in Van Province. Although extensive studies have been carried out in the world on the determination of the species of canine echinococcosis at the molecular level (3, 9, 21, 22), few researchers have used the copro-PCR test in Turkey.…”

Section: Discussionmentioning

confidence: 99%

“…Several methods have been applied for the accurate diagnosis of E. granulosus -infected dogs. Among these methods, copro-PCR for distinguishing species of taeniid eggs in faecal or environmental samples has begun to be used commonly (1, 3, 9, 20, 21, 23). Although several PCR studies have indicated the prevalence of canine echinococcosis in Turkey (12, 16, 25, 33, 34), PCR-based molecular analysis of canine echinococcosis is still rather limited (2, 19, 23).…”

Section: Introductionmentioning

confidence: 99%

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Preliminary studies on the prevalence and genotyping of Echinococcus granulosus infection in stray dogs in Van Province, Turkey

Oğuz

Özdal

Kılınç

et al. 2018

Journal of Veterinary Research

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IntroductionEchinococcus granulosus is a zoonotic helminth of the Taeniidae family living in the small intestines of dogs. The hydatid cyst, which is the larval form of this parasite, is observed in sheep, goat, cattle, and many other organisms including humans. It causes a disease called cystic echinococcosis. Identification of strains of E. granulosus in dogs is critical in parasite control and eradication where possible. This study aims to determine the genotype of E. granulosus eggs and prevalence of this parasite in the faeces of dogs in the Van Province using the copro-PCR method.Material and MethodsThis study was conducted between 2015 and 2016 on the faeces obtained from 100 stray dogs from different parts of the Van Province. The coprological examination was conducted using the formalin-ether concentration method.ResultsTaeniidae eggs were found in 10 (10%) out of 100 faecal samples. E. granulosus was detected in 4 out of 10 of these (40%) infected samples. Sequence analysis of positive amplicons obtained from PCR showed that there were sheep strains (G1).ConclusionDogs in Van area are primarily infected with the livestock genotype of E. granulosus, which is thought to be a potential zoonotic threat to humans.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Preliminary studies on the prevalence and genotyping of Echinococcus granulosus infection in stray dogs in Van Province, Turkey

Oğuz

Özdal

Kılınç

et al. 2018

Journal of Veterinary Research

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“…Cystic echinococcosis (CE), a major zoonotic helminth infection, imposes a socioeconomic burden in many countries all over the world, including Iran [1][2][3][4][5]. The metacestode of Echinococcus granulosus harbors in the viscera of wide variety of herbivores, including sheep, goats, cattle, and camel.…”

Section: Introductionmentioning

confidence: 99%

Epidemiological and molecular studies on Echinococcus granulosus from free-roaming dogs in Southeast Iran

et al. 2020

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Background and Aim: Cystic echinococcosis (CE), as a major zoonotic helminth infection, imposes remarkable socioeconomic burden on many endemic countries across the world, including Iran. Due to the high importance of free-roaming dogs in the transmission of CE, epidemiological and molecular studies in this type of hosts are required in the endemic regions. This study aimed to investigate the epidemiology and genotyping of Echinococcus granulosus isolated from stray dogs in Kerman, Southeast Iran. Materials and Methods: Eighty-four samples were isolated from stray dogs in the city and suburbs of Kerman in coordination with the health authorities and the municipality office for rabies control and dog population management. Dog demographic data, including age and sex were collected. The worm was isolated by necropsy and genomic DNA was extracted and partial cytochrome c oxidase subunit 1 gene was amplified using specific primers. Phylogenetic and Templeton-Crandall-Sing (TCS) network analyses were carried out on the sequence data. Results: The overall prevalence of CE in the surveyed dogs was 10.7% (9/84 cases). Out of 84 stray dogs, 33 (39.3%) and 51 (60.7%) cases were male and female, respectively. There was not a statistically significant difference between the infection and gender of dogs. However, infection is shown more in dogs under one year of age with a statistically significant difference (p<0.05). The results of molecular studies indicated E. granulosus G1 genotype for all isolates. The high presence of free-roaming dogs in urban and peri-urban areas and high frequency of parasite in this animal is a risk factor for humans in the region. Haplotype sequence analysis on the dog isolates revealed a close relationship with other E. granulosus isolates in Kerman. Conclusion: The findings of this study provide evidence-based data about the epidemiological and molecular characteristics of CE in dog definitive hosts of Southeast Iran. Further studies are required to understand the prevalence and parasite genotypes in dogs in Iran.

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“…Dogs have been found frequently infected with these tapeworm species. For E. granulosus , the prevalence ranges from 3.6% to 25.9% in Asia, Europe and Africa using molecular tools 4 . It has been shown that T. hydatigena is one of the most prevalent Taenia species in dogs and sheep in Iran, reported in 28.5-53.0% of dogs, 9.1% of foxes, 10% of jackals and 12.8% of sheep in different parts of the country 5 - 7 .…”

Section: Introductionmentioning

confidence: 99%

A conventional PCR for differentiating common taeniid species of dogs based on in silico microsatellite analysis

Shamsaddini

Mohammadi

Mirbadie

et al. 2017

Rev. Inst. Med. trop. S. Paulo

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Canine taeniids are among the major tapeworms with remarkable medical and economic significance. Reliable diagnosis and differentiation of dog taeniids using simple and sensitive tools are of paramount importance for establishing an efficient surveillance system. Microsatellites as abundant unique tandem repeats of short DNA motifs are useful genetic markers for molecular epidemiological studies. The purpose of the present study was to find a primer pair for rapid differentiation of major tapeworms of dogs, Taenia hydatigena, T. multiceps, T. ovis and Echinococcus granulosus, by screening existing nucleotide data. All the mitochondrial genome records as well as non-coding ITS1 sequences of Taeniidae species were downloaded from Nucleotide database from NCBI. For prediction and analysis of potential loci of STR/SSR in ITS1 as well as mitochondrial regions, we used ChloroMitoSSRDB 2.0 and GMATo v1.2. software. Different tapeworm species were categorized according to different motif sequences and type and size of each microsatellite locus. Three primer sets were designed and tested for differentiating taeniid species and evaluated in a conventional PCR system. Four taeniid species were successfully differentiated using a primer pair in a simple conventional PCR system. We predicted 2-19 and 1-4 microsatellite loci in ITS1 and mitochondrial genome, respectively. In ITS1, 41 Di and 21 Tri motifs were found in the taeniids while the majority of the motifs in the mitochondrial genome were Tetra (89) and Tri (70). It is documented that the number and diversity of microsatellite loci is higher in nuclear ITS1 region than mostly coding mitochondrial genome.

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Copro-PCR prevalence of Echinococcus granulosus infection in dogs in Kerman, south-eastern Iran

Cited by 11 publications

References 22 publications

Preliminary studies on the prevalence and genotyping of Echinococcus granulosus infection in stray dogs in Van Province, Turkey

Preliminary studies on the prevalence and genotyping of Echinococcus granulosus infection in stray dogs in Van Province, Turkey

Epidemiological and molecular studies on Echinococcus granulosus from free-roaming dogs in Southeast Iran

A conventional PCR for differentiating common taeniid species of dogs based on in silico microsatellite analysis

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