Coproduction of protease and mannanase from Bacillus nealsonii PN-11 in solid state fermentation and their combined application as detergent additives

David, Aditi; Chauhan, Prakram Singh; Kumar, Aditya; Angural, Steffy; Kumar, Deepak; Puri, Neena; Gupta, Naveen

doi:10.1016/j.ijbiomac.2017.09.037

Cited by 56 publications

(27 citation statements)

References 23 publications

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“…Proteases are among the first enzymes used by humans in biotechnological food processing [1,2]. Nowadays, proteases are used in many different areas, for example in the production of active peptides from inexpensive proteins, to improve the organoleptic or functional properties of foods [3], although perhaps its main use is as detergent components [4][5][6][7]. In some instances, a chemical alternative exists [8] but proteases have clear advantages, as the process will be more specific, avoiding the production of by-products or destruction of some amino acids [9].…”

Section: Introductionmentioning

confidence: 99%

Immobilization/Stabilization of Ficin Extract on Glutaraldehyde-Activated Agarose Beads. Variables That Control the Final Stability and Activity in Protein Hydrolyses

et al. 2018

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Ficin extract has been immobilized on different 4% aminated-agarose beads. Using just ion exchange, immobilization yield was poor and expressed activity did not surpass 10% of the offered enzyme, with no significant effects on enzyme stability. The treatment with glutaraldehyde of this ionically exchanged enzyme produced an almost full enzyme inactivation. Using aminated supports activated with glutaraldehyde, immobilization was optimal at pH 7 (at pH 5 immobilization yield was 80%, while at pH 9, the immobilized enzyme became inactivated). At pH 7, full immobilization was accomplished maintaining 40% activity versus a small synthetic substrate and 30% versus casein. Ficin stabilization upon immobilization could be observed but it depended on the inactivation pH and the substrate employed, suggesting the complexity of the mechanism of inactivation of the immobilized enzyme. The maximum enzyme loading on the support was determined to be around 70 mg/g. The loading has no significant effect on the enzyme stability or enzyme activity using the synthetic substrate but it had a significant effect on the activity using casein; the biocatalysts activity greatly decreased using more than 30 mg/g, suggesting that the near presence of other immobilized enzyme molecules may generate some steric hindrances for the casein hydrolysis.

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Section: Introductionmentioning

confidence: 99%

Immobilization/Stabilization of Ficin Extract on Glutaraldehyde-Activated Agarose Beads. Variables That Control the Final Stability and Activity in Protein Hydrolyses

et al. 2018

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“…Although many Bacillus strains were tested previously, it is clear that numerous species remain undiscovered and not officially described [ 55 , 56 ]. B. nealsonii was already isolated from potentially hostile environments, including a spacecraft assembly and the rumen of a buffalo [ 57 , 58 ], and it is not well characterized or shown great usefulness except for its very limited application to the production of proteases and as an additive detergent [ 59 , 60 ]. The secondary metabolites of the strain Sen132 of B. nealsonii that we isolated were screened for mosquitocidal activity and exhibited high activity against A. albopictus larvae.…”

Section: Discussionmentioning

confidence: 99%

Biological Control of Aedes albopictus: Obtained from the New Bacterial Candidates with Insecticidal Activity

Dahmana

Sambou

Raoult

et al. 2020

Insects

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Vector-borne deadly pathogens cause more than 700,000 deaths annually. They are transmitted by several vectors, among which the mosquito is the most important. Chemical compounds often have devastating side effects, leading to the abandonment of the majority of them. Biological control has been performed by using formulations of Bacillus sphaericus and Bacillus thuringiensis, but their intensive use has led to the emergence of resistance. Currently, the development of new alternative molecules is urgently needed, in order to use them in mosaics or in rotation with already known insecticides for the control of vectors, especially mosquitoes. Here, we attempted to identify bacterial species with potential anti-mosquito actions. Among bacterial strains isolated from dry sandy soil from Senegal, eleven strains from the Bacillales and Actinomycetales orders were chosen for the entomopathogenic activity experiments. Then, we tested their secondary metabolites, which were obtained from the supernatant fraction, and their cell wall and cytoplasmic compounds, which were found in the pellet fraction, in Aedes albopictus larvae, and compared the larval mortality rate with that obtained by using a commercial product. A total of 4/11 (36.36%) of the isolated species exhibited insecticidal activity. B. nealsonii, which is not a well-known bacterium, had the highest larvicidal effect with 70% of the larval mortality, which is highlighted for the first time. The Streptomyces species we isolated seem to be potential new species, and 3/5 (60%) of them exhibited insecticidal activity. Our study reports provide potential candidates for the identification of active molecules to be developed for strengthening the biological control of infectious diseases agents transmitted by mosquitoes.

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“…Although many Bacillus strains have already been tested, it is clear that numerous species have not yet been discovered and officially described 48,49 . B. nealsonii has already been isolated from potentially hostile environments, including a spacecraft assembly and the rumen of a buffalo 50,51 , and it has not been well characterised or shown great usefulness except for its very limited application to the production of proteases and as an additive detergent 52,53 . The secondary metabolites of the strain Sen132 of B.…”

Section: Discussionmentioning

confidence: 99%

Insecticidal activity of the metabolites of sand soil bacteria in Northern Senegal

Dahmana¹,

Sambou²,

Raoult³

et al. 2020

Preprint

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BackgroundVector-borne deadly diseases cause more than 700,000 deaths annually. They are transmitted by several vectors, among which the mosquito is the most important. Chemical compounds often have devastating side effects, which has led to the abandonment of the majority of them. Biological control has been performed by using formulations of Bacillus sphaericus and Bacillus thuringiensis, but their intensive use has led to the emergence of resistance. Currently, there is an urgent need to develop new alterative molecules from bacteria to use in mosaics or in rotation with already known insecticides and bacteria for the control of vectors, especially mosquitos. Here, we attempted to identify bacterial species with potential anti-mosquito actions.ResultsAmong 208 strains isolated from dry sandy soil from Senegal, eleven bacterial strains from the Bacillus genus (5) and Actinomycetales order (6) were chosen for the following experiments. Then, we tested their secondary metabolites, which were obtained from the supernatant fraction, and their cell wall and cytoplasmic compounds, which were found in the pellet fraction, in 3rd and early 4th Aedes albopictus larvae instars and compared the larval mortality rate with that obtained by using a commercial product. A total of 4/11 (36.4%) of the isolated species exhibited insecticidal activity. B. nealsonii, which is not a well-known bacterium, had the highest larvicidal effect with 70% larval mortality, which highlighted for the first time. The Streptomyces spp. we isolated seem to be potential new species, and 3/5 (60%) of them exhibited insecticidal activity.ConclusionThe bacterial strains isolated in this study may be used to identify the molecular mechanisms of their insecticidal activity.

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Coproduction of protease and mannanase from Bacillus nealsonii PN-11 in solid state fermentation and their combined application as detergent additives

Cited by 56 publications

References 23 publications

Immobilization/Stabilization of Ficin Extract on Glutaraldehyde-Activated Agarose Beads. Variables That Control the Final Stability and Activity in Protein Hydrolyses

Immobilization/Stabilization of Ficin Extract on Glutaraldehyde-Activated Agarose Beads. Variables That Control the Final Stability and Activity in Protein Hydrolyses

Biological Control of Aedes albopictus: Obtained from the New Bacterial Candidates with Insecticidal Activity

Insecticidal activity of the metabolites of sand soil bacteria in Northern Senegal

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