Copurification of chicken liver soluble thiamine monophosphatase and low molecular weight acid phosphatase

Kolas, I. K.; Makarchikov, Alexander F

doi:10.15407/ubj89.06.013

Cited by 3 publications

(4 citation statements)

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“…Thus, AThTP hydrolase has a quite high apparent affinity for substrate, like that of the specific soluble mammalian ThTPase (k m = 20-50 µМ [23]). For comparison, the k m values of non-specific phosphatases which might be involved in thiamine metabolism were reported to be 0.9 mM for ThMPase from chicken liver [24], 17-20.7 mM for ThDPase (NDPase) from rat and bovine liver [19,20], 2 mM for ThDPase (NDPase) from rat brain [20], 1.5-2.2 mM for a membrane-associated ThTPase in various animal species [25][26][27].…”

Section: Resultsmentioning

confidence: 99%

Adenosine thiamine triphosphate and adenosine thiamine triphosphate hydrolase activity in animal tissues

Makarchikov¹,

Saroka²,

Kudyrka³

2018

Ukr.Biochem.J.

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Adenosine thiamine triphosphate (AThTP), a vitamin B 1 containing nucleotide with unknown biochemical role, was found previously to be present in various biological objects including bacteria, yeast, some human, rat and mouse tissues, as well as plant roots. In this study we quantify AThTP in mouse, rat, bovine and chicken tissues. We also show that in animal tissues the hydrolysis of AThTP is catalyzed by a membranebound enzyme seemingly of microsomal origin as established for rat liver, which exhibits an alkaline ph optimum of 8.0-8.5 and requires no Mg 2+ ions for activity. In liver homogenates, AThTP hydrolase obeys Michaelis-Menten kinetics with apparent k m values of 84.4 ± 9.4 and 54.6 ± 13.1 µМ as estimated from the hanes plots for rat and chicken enzymes, respectively. The hydrolysis of AThTP has been found to occur in all samples examined from rat, chicken and bovine tissues, with liver and kidney being the most abundant in enzyme activity. In rat liver, the activity of AThTP hydrolase depends on the age of animals. k e y w o r d s: vitamin B 1 , adenosine thiamine triphosphate, adenosine thiamine triphosphate hydrolase, animal tissues.

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Section: Resultsmentioning

confidence: 99%

Adenosine thiamine triphosphate and adenosine thiamine triphosphate hydrolase activity in animal tissues

Makarchikov¹,

Saroka²,

Kudyrka³

2018

Ukr.Biochem.J.

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“…Активность тиаминмоно-(ТМФ-аза), ди-(ТДФ-аза) и три (ТТФ-аза) фосфатаз -по высвобождению неорганического фосфата (РН) [5,6]. Концентрацию РН измеряли колориметрически [7]. Проявляющиеся при дефосфоролизе ТМФ активности кислой и щелочной гидролаз, помимо РН, параллельно оценивали по накоплению п-нитрофенола, фиксируя оптическую плотность при 405 нм.…”

Section: материал и методыunclassified

“…Регистрируемая скорость гидролитической ТМФ-азной реакции Гепатология и гастроэнтерология № 2, 2021 при этом невысока и на порядок уступает скоростям дефосфорилирования тиаминди-и трифосфатов. Характерно, что из производных тиамина ТМФ единственная фосфорилированная форма, которая образуется исключительно при дефосфоролизе ТДФ: никаких специализированных путей или ферментов биосинтеза монофосфата в клетках животных и человека не обнаружено [7]. Во время операции, по мере нарастания стрессорной составляющей (на этапах максимальной травматичности операционного воздействия и экстубации), концентрация монофосфорного эфира быстро снижается при параллельной активации монофосфатазы.…”

Section: результаты и обсуждениеunclassified

“…Таким образом, ТМФ служит физиологическим субстратом фермента, биохимические функции которого состоят в дефосфорилировании флавинмононуклеотида и нескольких белков, участвующих в процессах роста, дифферен-цировки и адгезии клеток [7,13] того, что β-клетки инсулоцитов испытывают хронический дефицит SH-групп, расходуемых на биосинтез инсулина, а витамин В1 in vivo, благодаря антиоксидантным свойствам, повышает тканевый уровень восстановленных тиолов, то именно в поджелудочной железе эти свойства могут наиболее полно реализовываться через SH-зависимое увеличение гормоносинтеза [14].…”

Section: примечание: активность т-киназы и тдф-киназы выражали в нмоль•ч-1•мг-1 и в пмоль•ч-1•мг-1 соответственно тдф -эффект -в тех же еunclassified

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Vitamin B1 Biological Function Implementation in the Blood of Patients With Stomach Cancer Under Surgical Intervention

Kostenevich¹,

Черникевич²,

Baum³

et al. 2021

Hepatol and Gastroenterol

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Background. The probability of undergoing surgery always predetermines the state of stress in a person; therefore, it is advisable to search for ways to optimize and (or) reduce this unfavourable effect. Objective. To find out the mechanism of vitamin B1 antistress activity during surgery. Material and methods. Metabolism intensity was investigated on donors’ blood lysates (n = 19) and those of patients with stage III stomach cancer (n = 64), referred to an elective surgery, aged 51-70. The blood was taken from the cubital vein three days before the operation, after premedication, during the most traumatic moment of the operation, after extubation, as well as on the first and third days of the postoperative period. The surgery was performed under combined multicomponent anesthesia using nitric oxide, sodium hydroxybutyrate, and epidural block. Thiamine and thiamine diphosphate kinase activities were assessed by the concentration of the formed thiamine di- and triphosphates of the vitamin. The activities of thiamine mono-, di- and triphosphatases were determined by the release of inorganic phosphate. The concentration of inorganic phosphate was recorded colorimetrically. The content of B1 and its derivatives in the blood was determined by the method of ion-pair reversed-phase HPLC. Results. There has been observed an increased content of thiamine monophosphate and that of free thiamine in the blood of donors and patients with stomach cancer. The registered rate of the hydrolytic thiamine monophosphatase reaction is not high. At the stages of premedication and maximum trauma of surgical exposure, the concentration of monophosphoric ester rapidly decreases alongside with monophosphatase activation. Therefore, the thiamine monophosphate hydrolysis is the rate-limiting link of vitamin B1 metabolism. The level of free thiamine remains persistently increased at all stages of surgical treatment. Thiamine monophosphatase activity is manifested at two pH optima – of 6.0 and 9.0. Thiamine monophosphate hydrolysis at pH of 9.0 is catalyzed by alkaline phosphatase. At pH of 6.0, in addition to thiamine monophosphoric ester, the enzyme hydrolyzes only p-nitrophenyl phosphate, flavin mononucleotide and phosphotyrosine, that allows it to be classified as hepatic acid phosphatase. The noted changes in B1 metabolism under stress concern mainly non-coenzyme forms - thiamine mono-, triphosphate, and free thiamine, which are used at the stages of thiol reduction as important components of insulin synthesis. Conclusions. The use of vitamin B1 allows to optimize the development of the stress response at all stages of surgical treatment. Its protective effect is achieved through the activation of the insulin-synthetic function of the pancreas, which increases the level of immunoreactive insulin in the blood. The formation of the most favorable physiological conditions for insulin synthesis provides an increased background of free thiamine, which is created due to the hydrolysis of noncoenzyme forms of the vitamin. The relationship between thiamine metabolism and B2 exchange and regulation of intracellular signaling pathways has been traced.

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Synthesis, physico-chemical properties and effect of adenosine thiamine triphosphate on vitamin B1 metabolism in the liver of alloxan diabetic rats

Makarchikov

Kudyrka

Luchko

et al. 2022

Biochimica et Biophysica Acta (BBA) - General Subjects

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Copurification of chicken liver soluble thiamine monophosphatase and low molecular weight acid phosphatase

Cited by 3 publications

References 34 publications

Adenosine thiamine triphosphate and adenosine thiamine triphosphate hydrolase activity in animal tissues

Adenosine thiamine triphosphate and adenosine thiamine triphosphate hydrolase activity in animal tissues

Vitamin B1 Biological Function Implementation in the Blood of Patients With Stomach Cancer Under Surgical Intervention

Synthesis, physico-chemical properties and effect of adenosine thiamine triphosphate on vitamin B1 metabolism in the liver of alloxan diabetic rats

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