2018
DOI: 10.3389/fgene.2018.00306
|View full text |Cite
|
Sign up to set email alerts
|

Copy Number of Human Ribosomal Genes With Aging: Unchanged Mean, but Narrowed Range and Decreased Variance in Elderly Group

Abstract: Introduction: The multi-copied genes coding for the human 18, 5.8, and 28S ribosomal RNA (rRNA) are located in five pairs of acrocentric chromosomes forming so-called rDNA. Human genome contains unmethylated, slightly methylated, and hypermethylated copies of rDNA. The major research question: What is the rDNA copy number (rDNA CN) and the content of hypermethylated rDNA as a function of age?Materials and Methods: We determined the rDNA CN in the blood leukocyte genomes of 651 subjects aged 17 to 91 years. The… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

5
55
0
2

Year Published

2018
2018
2023
2023

Publication Types

Select...
6
2
1

Relationship

0
9

Authors

Journals

citations
Cited by 55 publications
(62 citation statements)
references
References 50 publications
5
55
0
2
Order By: Relevance
“…Aging has been linked to rDNA loss in yeast, and the application of that phenomenon to humans has been proposed and, in some cases, supported (Malinovskaya et al 2018). In contrast to those studies, we could detect no correlation between individual rDNA copy number or C.V. with age at time of blood collection ( Figure 7C).…”
Section: Rdna Copy Numbers Do Not Correlate With Age Sex or Ethnicitymentioning
confidence: 61%
See 1 more Smart Citation
“…Aging has been linked to rDNA loss in yeast, and the application of that phenomenon to humans has been proposed and, in some cases, supported (Malinovskaya et al 2018). In contrast to those studies, we could detect no correlation between individual rDNA copy number or C.V. with age at time of blood collection ( Figure 7C).…”
Section: Rdna Copy Numbers Do Not Correlate With Age Sex or Ethnicitymentioning
confidence: 61%
“…Our collection of blood from breast cancer patients, and from patients with non-cancer diagnoses, allowed us to ascertain deviations in average rDNA copy number as a consequence of age, sex, and ethnicity. These data could prove useful in evaluating hypotheses linking rDNA copy number to age (Malinovskaya et al 2018;Wang and Lemos 2019), disease status, or involvement in lifestyle choices that lead to greater disease risk (e.g., alcohol consumption, cigarette smoking, overeating (Holland et al 2016)). We re-analyzed the rDNA copy number from the non-cancer-diagnosis individuals from the Tucson area and this time looked for correlations between average rDNA copy number and sex and ethnicity as categorical conditions ( Figure 7A-B).…”
Section: Rdna Copy Numbers Do Not Correlate With Age Sex or Ethnicitymentioning
confidence: 99%
“…Blocking of replication forks in the region of ribosomal RNAs genes leads to the activation of repair processes in which rDNA has a major initiator role, and the disruption of the mechanism of rDNA-dependent conducting of DNA repair leads to the inducement of repeat instability [43]. Altogether, ribosomal RNA coding regions in the human genome tend to be extremely unstable: the copy number of 45S rRNA genes may change with aging [44] and under different conditions [45], it differs in healthy individuals and patients with a disease [46], and can be both increased and decreased in cancers [25]. Induced by improper DNA repair, rDNA copy number variation is associated with global reorganization of gene expression and may contribute to cell's adaptation abilities [47].…”
Section: Discussionmentioning
confidence: 99%
“…The DNA from the spermatozoa was then extracted by a modified guanidinium thiocyanate method (Gazquez et al, 2008). Because of interindividual variation of rDNA, as well as variation with aging (Malinovskaya et al, 2018), genotyping of rDNA promoter region underwent targeted PCR analysis with Sanger sequencing. PCR primers were: Forward, TTCGGTCCCTCGTGTGTC; Reverse, GAGAGAACAGCAGGCCCG.…”
Section: Dna Isolation Genotyping and Pyrosequencingmentioning
confidence: 99%