2018
DOI: 10.1080/15592294.2017.1417710
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Cord blood buffy coat DNA methylation is comparable to whole cord blood methylation

Abstract: Cord blood DNA methylation is associated with numerous health outcomes and environmental exposures. Whole cord blood DNA reflects all nucleated blood cell types, while centrifuging whole blood separates red blood cells, generating a white blood cell buffy coat. Both sample types are used in DNA methylation studies. Cell types have unique methylation patterns and processing can impact cell distributions, which may influence comparability. We evaluated differences in cell composition and DNA methylation between … Show more

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Cited by 7 publications
(4 citation statements)
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“…The upper limit for the number of outlier CpGs in our dataset was identified to be 80,233; all three OMP individuals were significantly above that limit (189,873; 160,935; and 171,662, respectively). The OMP was confirmed for all three children when compared to upper and lower limits calculated from one external healthy cord blood dataset derived from 7 children (6 White, 1 Asian Indian, 1 Mixed-race) (GSE103189, [ 14 ]) and for two of three children (Children S and U) in another (GSE122288, unpublished data, race/ethnicity data not available). Furthermore, all three children appear to be outliers in hierarchical clustering analysis after excluding 74,747 XY-linked and SNP-linked CpGs (to prevent genetic sex and ancestry impacts on clustering) (Fig.…”
Section: Resultsmentioning
confidence: 67%
“…The upper limit for the number of outlier CpGs in our dataset was identified to be 80,233; all three OMP individuals were significantly above that limit (189,873; 160,935; and 171,662, respectively). The OMP was confirmed for all three children when compared to upper and lower limits calculated from one external healthy cord blood dataset derived from 7 children (6 White, 1 Asian Indian, 1 Mixed-race) (GSE103189, [ 14 ]) and for two of three children (Children S and U) in another (GSE122288, unpublished data, race/ethnicity data not available). Furthermore, all three children appear to be outliers in hierarchical clustering analysis after excluding 74,747 XY-linked and SNP-linked CpGs (to prevent genetic sex and ancestry impacts on clustering) (Fig.…”
Section: Resultsmentioning
confidence: 67%
“…To date, few studies have provided comparative analyses of different epigenetic profiles across different blood fractions in pregnancy. One notable example is a study of the cord blood, which showed that methylation in whole blood is reasonably comparable to buffy coat in a small number ( n = 8) of paired samples ( Dou et al, 2018 ). However, the concordance between special cell populations (such as lymphocytes or monocytes) with either whole blood or buffy coat was not studied.…”
Section: Discussionmentioning
confidence: 99%
“…As recommended by the calculator tutorial, we checked normalize data and the advanced analysis options to obtain the epigenetic ages. We used a previously verified method of using buffy coat methylation profile-type proportions as opposed to blood cell proportions for the calculations [37,38]. The variable used in the analyses, DNAmGrimAge-difference, was calculated by subtracting each individual's chronological age from their DNAmGrimAge.…”
Section: Dna Methylation Age Calculation For Grimagementioning
confidence: 99%