Cord Blood DNA Methylation of Treg Cytokine Genes Differs with Parity

Nilormee, Orpita; Lockett, Gabrielle A.; Iqbal, Sabrina; Holloway, John W.; Arshad, Syed Hasan; Karmaus, Wilfried

doi:10.1016/j.jaci.2014.12.1257

Cited by 1 publication

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“…All the analyses were adjusted for birthweight, 40 parity, 41 gestational age 40 , 42 and sex of the newborn, 43 in addition to technical variables: bead array row and bisulphite conversion batch.…”

Section: Methodsmentioning

confidence: 99%

Socioeconomic position during pregnancy and DNA methylation signatures at three stages across early life: epigenome-wide association studies in the ALSPAC birth cohort

Alfano

Guida

Galobardes

et al. 2018

International Journal of Epidemiology

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Background Socioeconomic experiences are recognized determinants of health, and recent work has shown that social disadvantages in early life may induce sustained biological changes at molecular level that are detectable later in life. However, the dynamics and persistence of biological embedding of socioeconomic position (SEP) remains vastly unexplored. Methods Using the data from the ALSPAC birth cohort, we performed epigenome-wide association studies of DNA methylation changes at three life stages (birth, n = 914; childhood at mean age 7.5 years, n = 973; and adolescence at mean age 15.5 years, n = 974), measured using the Illumina HumanMethylation450 Beadchip, in relation to pregnancy SEP indicators (maternal and paternal education and occupation). Results Across the four early life SEP metrics investigated, only maternal education was associated with methylation levels at birth, and four CpGs mapped to SULF1, GLB1L2 and RPUSD1 genes were identified [false discovery rate (FDR)-corrected P -value <0.05]. No epigenetic signature was found associated with maternal education in child samples, but methylation levels at 20 CpG loci were found significantly associated with maternal education in adolescence. Although no overlap was found between the differentially methylated CpG sites at different ages, we identified two CpG sites at birth and during adolescence which are 219 bp apart in the SULF1 gene that encodes an heparan sulphatase involved in modulation of signalling pathways. Using data from an independent birth cohort, the ENVIR ON AGE cohort, we were not able to replicate these findings. Conclusions Taken together, our results suggest that parental SEP, and particularly maternal education, may influence the offspring’s methylome at birth and adolescence.

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