Cord blood-hematopoietic stem cell expansion in 3D fibrin scaffolds with stromal support

Ferreira, Mónica S. Ventura; Jahnen-Dechent, Willi; Labude, Norina; Bovi, Manfred; Hieronymus, Thomas; Zenke, Martin; Schneider, Rebekka K.; Neurs, Sabine

doi:10.1016/j.biomaterials.2012.06.029

Cited by 152 publications

(61 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Analysis of the absolute percentage growth of CD34+ cells during the time in culture demonstrated that the 3D architecture did not significantly impact this outcome, which is in agreement with what has been reported with other types of 3D culture systems 51, 52.…”

Section: Discussionsupporting

confidence: 89%

“…The importance of the composition and architecture of the microenvironment in inducing cell division/differentiation or expansion of CB‐HSPC has been demonstrated by the use of mixed feeder layers within the same 2D culture 47, acellular matrices 48, and 3D culture systems 49, 50, 51, 52. Nevertheless, the challenge of expanding long‐term repopulating HSC from a single unit, in such a way that sufficient numbers of HSC will be obtained to engraft an adult, while simultaneously increasing progenitor populations that are able to shorten time to engraftment, has yet to be solved 1, 2.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluating Interaction of Cord Blood Hematopoietic Stem/Progenitor Cells with Functionally Integrated Three-Dimensional Microenvironments

Mokhtari

Baptista

Vyas

et al. 2018

Stem Cells Translational Medicine

View full text Add to dashboard Cite

Despite advances in ex vivo expansion of cord blood‐derived hematopoietic stem/progenitor cells (CB‐HSPC), challenges still remain regarding the ability to obtain, from a single unit, sufficient numbers of cells to treat an adolescent or adult patient. We and others have shown that CB‐HSPC can be expanded ex vivo in two‐dimensional (2D) cultures, but the absolute percentage of the more primitive stem cells decreases with time. During development, the fetal liver is the main site of HSPC expansion. Therefore, here we investigated, in vitro, the outcome of interactions of primitive HSPC with surrogate fetal liver environments. We compared bioengineered liver constructs made from a natural three‐dimensional‐liver‐extracellular‐matrix (3D‐ECM) seeded with hepatoblasts, fetal liver‐derived (LvSt), or bone marrow‐derived stromal cells, to their respective 2D culture counterparts. We showed that the inclusion of cellular components within the 3D‐ECM scaffolds was necessary for maintenance of HSPC viability in culture, and that irrespective of the microenvironment used, the 3D‐ECM structures led to the maintenance of a more primitive subpopulation of HSPC, as determined by flow cytometry and colony forming assays. In addition, we showed that the timing and extent of expansion depends upon the biological component used, with LvSt providing the optimal balance between preservation of primitive CB HSPC and cellular differentiation. Stem Cells Translational Medicine 2018;7:271–282

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Evaluating Interaction of Cord Blood Hematopoietic Stem/Progenitor Cells with Functionally Integrated Three-Dimensional Microenvironments

Mokhtari

Baptista

Vyas

et al. 2018

Stem Cells Translational Medicine

View full text Add to dashboard Cite

show abstract

“…Indeed, fibrin has been clinically employed as a safe and versatile biomaterial for stimulating and accelerating wound healing and tissue regeneration in numerous medical conditions [593,611,617]. Interestingly, recent evidence suggests that a 3D fibrin scaffold may mimic the main components of the hematopoietic niche, thus meeting the optimal requirements of clinical protocols for cord blood-hematopoietic stem cell expansion and transplantation [618]. …”

Section: Formulations Enriched With Endogenous Growth Factorsmentioning

confidence: 99%

Advancing biomaterials of human origin for tissue engineering

Chen

Liu

2016

Progress in Polymer Science

959

513

View full text Add to dashboard Cite

Biomaterials have played an increasingly prominent role in the success of biomedical devices and in the development of tissue engineering, which seeks to unlock the regenerative potential innate to human tissues/organs in a state of deterioration and to restore or reestablish normal bodily function. Advances in our understanding of regenerative biomaterials and their roles in new tissue formation can potentially open a new frontier in the fast-growing field of regenerative medicine. Taking inspiration from the role and multi-component construction of native extracellular matrices (ECMs) for cell accommodation, the synthetic biomaterials produced today routinely incorporate biologically active components to define an artificial in vivo milieu with complex and dynamic interactions that foster and regulate stem cells, similar to the events occurring in a natural cellular microenvironment. The range and degree of biomaterial sophistication have also dramatically increased as more knowledge has accumulated through materials science, matrix biology and tissue engineering. However, achieving clinical translation and commercial success requires regenerative biomaterials to be not only efficacious and safe but also cost-effective and convenient for use and production. Utilizing biomaterials of human origin as building blocks for therapeutic purposes has provided a facilitated approach that closely mimics the critical aspects of natural tissue with regard to its physical and chemical properties for the orchestration of wound healing and tissue regeneration. In addition to directly using tissue transfers and transplants for repair, new applications of human-derived biomaterials are now focusing on the use of naturally occurring biomacromolecules, decellularized ECM scaffolds and autologous preparations rich in growth factors/non-expanded stem cells to either target acceleration/magnification of the body's own repair capacity or use nature's paradigms to create new tissues for restoration. In particular, there is increasing interest in separating ECMs into simplified functional domains and/or biopolymeric assemblies so that these components/constituents can be discretely exploited and manipulated for the production of bioscaffolds and new biomimetic biomaterials. Here, following an overview of tissue auto-/allo-transplantation, we discuss the recent trends and advances as well as the challenges and future directions in the evolution and application of human-derived biomaterials for reconstructive surgery and tissue engineering. In particular, we focus on an exploration of the structural, mechanical, biochemical and biological information present in native human tissue for bioengineering applications and to provide inspiration for the design of future biomaterials.

show abstract

“…Several different approaches to culture and investigate HSCs in vitro using 3D systems have been tested up until now: HSCs †, § have been encapsulated in hydrogels made of natural (e.g., collagen, hyaluronic acid) or synthetic (e.g., polyacrylates) monomers; 48,50,52 Other approaches included seeding of HSCs † onto scaffolds made of fibres or fibre meshworks, 51 as well as culturing HSCs †, ‡ ‡ in macroporous scaffolds that resemble the spongy architecture of trabecular bones 49,[181][182][183] (these host the red, blood-forming bone marrow); Furthermore, microwell-arrays have been used as quasi-or pseudo-3D environments for HSCs § §, ¶ ¶ in order to delineate signals and parameters that are present in the HSC niche. [184][185][186][187] All of these studies showed that HSCs respond and act differently in 3D materials than in standard 2D culture, backing the assumption that conventional 2D culture is a highly artificial situation for HSCs, which may even induce 'unnatural' cell behaviour.…”

Section: D Versus 2dmentioning

confidence: 99%