Cord blood maternal microchimerism following unrelated cord blood transplantation

Kanaan, Sami B; Delaney, Colleen; Milano, Filippo; Scaradavou, Andromachi; Besien, Koen van; Allen, Judith; Lambert, Nathalie; Cousin, Emma; Hill, Joshua A.; Kahn, Elena; Forsyth, Alexandra M.; Sensoy, Oyku; Nelson, J. Lee

doi:10.1038/s41409-020-01149-x

Cited by 12 publications

(11 citation statements)

References 31 publications

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“…Detection of MMc at birth was evaluated by logistic regression with adjustment for the total number of gEq tested for each subject. Level of MMc at birth was evaluated with negative binomial regression, accounting for the number of microchimeric gEq detected as well as the total number of gEq assessed in each sample (7,28,71,72,74,75). This approach accounts for the non-normal distribution of MMc data as well as the large number of zeros (74).…”

Section: Methodsmentioning

confidence: 99%

Factors influencing maternal microchimerism throughout infancy and its impact on infant T cell immunity

Balle¹,

Armistead²,

Kiravu³

et al. 2022

Journal of Clinical Investigation

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Section: Methodsmentioning

confidence: 99%

Factors influencing maternal microchimerism throughout infancy and its impact on infant T cell immunity

Balle¹,

Armistead²,

Kiravu³

et al. 2022

Journal of Clinical Investigation

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“…Similarly, maternal cells can be found in different immune cell subsets from CB samples and in sorted hematopoietic progenitor cells (CD34+) with levels reaching up to 1.5% [4,5,13]. Allogeneic hematopoietic stem cells from CB samples are increasingly used as a curative treatment for many cancers and inherited non-malignant diseases [14], and the beneficial role of maternal cells in the fate of the CB transplant is increasingly evidenced [15].…”

Section: Introductionmentioning

confidence: 99%

Grandmaternal cells in cord blood

et al. 2021

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Background: During pregnancy a feto-maternal exchange of cells through the placenta conducts to maternal microchimerism (Mc) in the child and fetal Mc in the mother. Because of this bidirectional traffic of cells, pregnant women have also acquired maternal cells in utero from their mother and could transfer grandmaternal (GdM) cells to their child through the maternal bloodstream during pregnancy. Thus, cord blood (CB) samples could theoretically carry GdMMc. Nevertheless this has never been demonstrated. Methods: Using Human Leukocyte Antigen (HLA)-specific quantitative PCR assays on three-generation families, we were able to test 28 CB samples from healthy primigravid women for GdMMc in whole blood (WB) and isolated cells (PBMC, T, B, granulocytes, stem cells). Findings: Five CB samples (18%) had GdMMc which could not be confounded with maternal source, with quantities 100 fold lower than maternal Mc in WB and PBMC. Risk of aneuploidies and/or related invasive prenatal procedures significantly correlated with the presence of GdMMc in CB (p=0.024). Significantly decreased HLA compatibility was observed in three-generation families from CB samples carrying GdMMc (p=0.019). Interpretation: Transgenerational transfer of cells could have implications in immunology and evolution. Further analyses will be necessary to evaluate whether GdMMc in CB is a passive or immunologically active transfer and whether invasive prenatal procedures could trigger GdMMc.

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“…The current consensus is that maternal cells are commonly detected in CB samples and amounts are significant (12). Moreover, maternal cells of the CB graft have been recently detected in 19% of 27 unrelated recipients post-CB transplantation (15). Maternal cells may be beneficial as recipients positive for MMc-CB tended to have lower relapse, mortality, and treatment failure than patients negative (15).…”

Section: Introductionmentioning

confidence: 99%

Factors Predicting the Presence of Maternal Cells in Cord Blood and Associated Changes in Immune Cell Composition

et al. 2021

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BackgroundCord blood (CB) samples are increasingly used as a source of hematopoietic stem cells in transplantation settings. Maternal cells have been detected in CB samples and their presence is associated with a better graft outcome. However, we still do not know what influences the presence of maternal microchimerism (MMc) in CB samples and whether their presence influences CB hematopoietic cell composition.Patients and MethodsHere we test whether genetic, biological, anthropometric and/or obstetrical parameters influence the frequency and/or quantity of maternal Mc in CB samples from 55 healthy primigravid women. Mc was evaluated by targeting non-shared, non-inherited Human Leukocyte Antigen (HLA)-specific real-time quantitative PCR in whole blood and four cell subsets (T, B lymphocytes, granulocytes and/or hematopoietic progenitor cells). Furthermore CB samples were analyzed for their cell composition by flow cytometry and categorized according to their microchimeric status.ResultsMMc was present in 55% of CB samples in at least one cell subset or whole blood, with levels reaching up to 0.3% of hematopoietic progenitor cells. Two factors were predictive of the presence of MMc in CB samples: high concentrations of maternal serological Pregnancy-Associated-Protein-A at first trimester of pregnancy (p=0.018) and feto-maternal HLA-A and/or –DR compatibility (p=0.009 and p=0.01 respectively). Finally, CB samples positive for MMc were significantly enriched in CD56+ cells compared to CB negative for MMc.ConclusionsWe have identified two factors, measurable at early pregnancy, predicting the presence of maternal cells in CB samples at delivery. We have shown that MMc in CB samples could have an influence on the hematopoietic composition of fetal cells. CD56 is the phenotypic marker of natural killer cells (NK) and NK cells are known to be the main effector for graft versus leukemia reactions early after hematopoietic stem cell transplantation. These results emphasize the importance of MMc investigation for CB banking strategies.

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Cord blood maternal microchimerism following unrelated cord blood transplantation

Cited by 12 publications

References 31 publications

Factors influencing maternal microchimerism throughout infancy and its impact on infant T cell immunity

Factors influencing maternal microchimerism throughout infancy and its impact on infant T cell immunity

Grandmaternal cells in cord blood

Factors Predicting the Presence of Maternal Cells in Cord Blood and Associated Changes in Immune Cell Composition

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