Cordycepin increases sensitivity of Hep3B human hepatocellular carcinoma cells to TRAIL-mediated apoptosis by inactivating the JNK signaling pathway

Lee, Hye Hyeon; Jeong, Jin‐Woo; Lee, Jun Hyuk; Kim, Gi‐Young; Cheong, JaeHun; Jeong, Yong Kee; Yoo, Young Hyun; Choi, Yung Hyun

doi:10.3892/or.2013.2589

Cited by 38 publications

(15 citation statements)

References 39 publications

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“…Traditional Chinese medicine is one of them for complementary and alternative therapy. Cordycepin, an active compound extracted from one Traditional Chinese medical herb, Cordyceps militaris, has been studied to treat many cancers including lung cancer (50), ovarian cancer (46), leukemia (30), breast cancer (47), brain cancer (19), colon cancer (27), liver cancer (25), and so on (33). Recently, some efforts have been done to investigate the po- PTEN/PI3K/Akt signaling pathway is an important pathway regulated by microRNA-21 (7).…”

Section: Discussionmentioning

confidence: 99%

Cordycepin induces apoptotic cell death and inhibits cell migration in renal cell carcinoma via regulation of microRNA-21 and PTEN phosphatase

et al. 2017

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Cordycepin is an active component extracted from Traditional Chinese medical herb Cordyceps militaris. Many reports demonstrated that cordycepin harbors antitumor activity in a broad spectrum of cancer types. In this study the actions and the underneath molecular mechanisms of cordycepin were investigated in renal cell carcinoma Caki-1 cell line. Results showed that cordycepin induced apoptotic cell death and inhibited cell migration in Caki-1 cells. Quantitative real-time PCR results and western blot analyses indicated cordycepin dose-dependently decreased microRNA-21 expression and Akt phosphorylation levels in Caki-1 cells, but increased PTEN phosphatase levels. Block of cordycepin-induced microRNA-21 decrease or PTEN increase in Caki-1 cells by transfection of microRNA-21 mimic or PTEN siRNA significantly attenuated cordycepin-induced cell death and inhibition of cell migration. Taken together, findings in present study suggested that cordycepin induced apoptotic cell death in renal cell carcinoma through regulation of microRNA-21 and PTEN phosphatase. Furthermore, present study also firstly illustrated that cordycepin inhibited cell migration of renal cell carcinoma, which also involved micro-RNA-21 and PTEN phosphatase.Renal cell carcinoma (RCC) is the most lethal urological cancer, accounting for 87% of all renal malignancies and 2-3% of all cancers (4). In the past two decades, the global incidence of RCC has increased by 2% per year (4). Worldwide, the agestandardized incidence and mortality of RCC were 6 per 100,000 and 2.1 per 100,000 respectively (43). Despite advance in diagnostic techniques, up to 20-30% of patients with RCC present with metastasis (4). The prognosis of RCC has historically been poor, with 5-year survival rate of 8% among patients with metastatic RCC (9). Although recent progress about understanding of the pathogenesis of RCC has led to novel immune-based and targeted treatments for this chemoresistant cancer, no one drug obtains overall response rate of 100% and durable complete responses (4, 9). Development of neo-adjuvant therapy is still in a demand at present. Cordycepin is a main active composition extracted from Cordyceps militaris and has been reported to harbor anti-tumor activity in a broad spectrum of cancer types, including lung cancer (50), ovarian cancer (46), leukemia (30), breast cancer (47), and so on (33). Two recent reports firstly demonstrated that cordycepin could induce apoptosis in renal cell carcinoma through Erk-JNK signaling pathway (20,48) and indicated the potential application of cordycepin in treating renal cell carcinoma. However, the actions and the underneath molecular mechanisms of cordycepin in renal cell carcinoma are not definitely clear and warrant extensive and further investigations. Recently, a comprehensive molecular analysis

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Section: Discussionmentioning

confidence: 99%

Cordycepin induces apoptotic cell death and inhibits cell migration in renal cell carcinoma via regulation of microRNA-21 and PTEN phosphatase

et al. 2017

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“…In general, those drugs often anti-metabolites that interfere with nucleic acid or protein synthesis can suppress the immune response. The greatest problem facing the physician is to effectively balance at the same time the dosages of drugs or irradiation so that the immune system is suppressed against the graft, but is still capable of defense against environmental pathogens [32][33][34][35][36][37][38][39][40][41][42].…”

Section: Acquired Immuno-suppressionmentioning

confidence: 99%

A Constitution Dependent QOL Assessment

Yamaguchi¹

2017

J Tradi Med Clin Natur

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According to the reports from Ministry of Health in each country, the average life spans were expanded over the past century. This trend is expected to be extended further in the future and at the same time continuous efforts should be made. That indicates that the quantitative objective, as one of the objectives people have aimed at since ancient times to attain the perpetual youth and longevity, are almost achieved in this way. However, as the next objective, it is necessary for us to be concerned about improving the more advanced quality of life.In an effort of improving the quality of life, other than the western medicine, we have attempted to bring many traditional medical practices, including the Oriental medicine, from various parts of the world into the medical field as alternative medicine. Judging both positive and negative aspects by the evaluation standard of the Western medicine, we try to obtain numerical values of QOL, and after that, set a numerical target for improvement of QOL. Some methods of alternative medicine taken in this review are acupuncture moxibustion, Chinese medicine and other traditional medicine. Above all, WHO suggests selecting the suzerain nation of acupuncture moxibustion, which has been developed in the Oriental countries and realized as a specialized cure, by raising good & bad points and ascertaining which nation advocating the best modeling method could be. At the moment for evaluating CAM, for example, what kind of methods is/are suitable for evaluating each CAM. We have been trying to propose the peripheral leukocyte is one of the best ways for evaluating CAM. Our trials are hot spring hydro therapy, acupuncture and moxibution, light exercise and undle heating. In these evaluations, we found the common results from leukocyte effects which exhibited strong correlation for the regulation. The contents are the result from the data showing the much in number tend to downly regulate, on the other hand lower number one is up-regulated. This kind of regulation showed within a 24 hrs, for the leukocyte subsets, granulocyte and lymphocyte are changed under circadian rhythm. So for purpose of evaluating leukocyte deviation, we set the point for evaluation at the same-time zone from the first set of evaluation. Under these condition, we got a same results reproductively that the whole number of leukocyte, and its subset, granulocyte and lymphocyte also regulated within a 24 hrs. This kind of phenomenon is the case that we confirm a lot of kind of CAM and repetition. However, the life span of leukocyte are at lease 3-4 days and no such a drastic apoptosis was induced such a stimulation. For the purpose of scientific explanation, we proposed that the emotional hormone are concern this change of leukocytes population. For the results of this hypothesis, there were reasonable change were seen in the peripheral blood about emotional hormone, adrenaline and dopamine. Other hormone concern thyroid grand was not changed significantly. In this review, I plan to collect evidence and...

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“…This result indicated that cordycepin induces tumor cell death through PKC/MAPK signaling pathways. Lee et al (38) performed Western blot analysis, which demonstrated that JNK-inactivating phosphatase was upregulated in response to treatment with cordycepin in human hepatocellular carcinoma Hep3B cells. Thus, according to the results of Pao et al (7) and Lee et al (38), we hypothesize that cordycepin induces cell apoptosis via the activation of PLC/PKC and subsequent inactivation of JNK, which is an important component of the MAPK and downstream PLC/PKC signaling pathways.…”

Section: Cell Apoptosis Induced By Cordycepinmentioning

confidence: 99%

“…Lee et al (38) performed Western blot analysis, which demonstrated that JNK-inactivating phosphatase was upregulated in response to treatment with cordycepin in human hepatocellular carcinoma Hep3B cells. Thus, according to the results of Pao et al (7) and Lee et al (38), we hypothesize that cordycepin induces cell apoptosis via the activation of PLC/PKC and subsequent inactivation of JNK, which is an important component of the MAPK and downstream PLC/PKC signaling pathways. Western blot analysis performed by Chen et al (39,40) also found that 24 h treatment with 10 or 100 µmol/l cordycepin exhibited a synergistically apoptotic effect through the activation of JNK/caspase-7/PARP pathway in human OC3 oral cancer cells.…”

Section: Cell Apoptosis Induced By Cordycepinmentioning

confidence: 99%

Apoptosis and inhibition of proliferation of cancer cells induced by cordycepin

Tian

Li²,

Shen³

et al. 2015

Oncology Letters

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Abstract. Cordycepin, a 3-deoxyadenosine, is the predominant functional component of the fungus Cordyceps militaris, a traditional Chinese medicine. Previous studies investigating the inhibition of cancer cells by cordycepin identified that it not only promotes cell apoptosis, but also controls cell proliferation. Furthermore, studies have elucidated the molecular mechanisms of inhibiting cell proliferation by cordycepin binding the A3 adenosine receptor, activating G protein, inhibiting cAMP formation, decreasing glycogen synthase kinase-3β/β-catenin activation and suppressing cyclin D1 and c-myc expression. The most significant signaling pathway in which cell apoptosis is induced by cordycepin is the caspase pathway. Cordycepin induces cell apoptosis via binding the DR3 receptor and consequently activating caspase-8/-3. Taken together, these studies demonstrate that cordycepin may be used as a natural medicine, as it can not only control tumor cell proliferation, but also induce cancer cell apoptosis.

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Cordycepin increases sensitivity of Hep3B human hepatocellular carcinoma cells to TRAIL-mediated apoptosis by inactivating the JNK signaling pathway

Cited by 38 publications

References 39 publications

<b>Cordycepin induces apoptotic cell death and inhibits cell migration in renal cell carcinoma via regulation of microRNA-21 and PTEN </b><b>phosphatase </b>

<b>Cordycepin induces apoptotic cell death and inhibits cell migration in renal cell carcinoma via regulation of microRNA-21 and PTEN </b><b>phosphatase </b>

A Constitution Dependent QOL Assessment

Apoptosis and inhibition of proliferation of cancer cells induced by cordycepin

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