Cordycepin induces apoptosis of C6 glioma cells through the adenosine 2A receptor-p53-caspase-7-PARP pathway

Chen, Ying; Yang, Shih‐Hung; Hueng, Dueng‐Yuan; Syu, Jhih-Pu; Liao, Chih-Chen; Wu, Ya‐Chieh

doi:10.1016/j.cbi.2014.03.010

Cited by 59 publications

(44 citation statements)

References 31 publications

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“…Lee and co-workers [20] suggested that cordycepin could inhibit the migration and invasion of LNCap human prostate carcinoma cells by inactivation of AKT, resulting in the down-regulation of the TJs (Tight Junctions) and MMPs (Matix Metalloproteinases). Chen and co-workers [21] investigated the effect of cordycepin on C6 glioma cells and showed that cordycepin could induce the apoptosis of C6 glioma cells via the adenosine 2A receptor-p53-caspase-7-PARP pathway. Though almost all the studies reported that cordycepin could inhibit cell proliferation and induce apoptosis, the concrete mechanisms were not identical.…”

Section: Introductionmentioning

confidence: 99%

Cordycepin Induces Apoptosis and Inhibits Proliferation of Human Lung Cancer Cell Line H1975 via Inhibiting the Phosphorylation of EGFR

Wang

Liang

et al. 2016

Molecules

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Abstract:Cordycepin is an active component of the traditional Chinese medicine Cordyceps sinensis and Cordyceps militaris with notable anticancer activity. Though the prominent inhibitory activity was reported in different kinds of cancer cell lines, the concrete mechanisms remain elusive. It was reported that cordycepin could be converted into tri-phosphates in vivo to confuse a number of enzymes and interfere the normal cell function. For the inhibitory mechanism of EGFR inhibitors and the structure similarity of ATP and tri-phosphated cordycepin, human lung cancer cell line H1975 was employed to investigate the inhibitory effect of cordycepin. The results showed that cordycepin could inhibit cell proliferation and induce apoptosis in a dose-dependent manner. Cell cycle analysis revealed that H1975 cells could be arrested at the G 0 /G 1 phase after cordycepin treatment. The expression levels of apoptosis-related protein Caspase-3 and Bcl-2 and phosphorylated expression levels of EGFR, AKT and ERK1/2 were all decreased compared with the control group stimulated with EGF. However, the protein expression levels of proapoptotic protein Bax and cleaved caspase-3 were increased. These results implied that cordycepin could inhibit cell proliferation and induce apoptosis via the EGFR signaling pathway. Our results indicated that there was potential to seek a novel EGFR inhibitor from cordycepin and its chemical derivatives.

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Section: Introductionmentioning

confidence: 99%

Cordycepin Induces Apoptosis and Inhibits Proliferation of Human Lung Cancer Cell Line H1975 via Inhibiting the Phosphorylation of EGFR

Wang

Liang

et al. 2016

Molecules

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“…PARP plays a pivotal role particularly in the maintenance of genomic DNA stability, apoptosis and in the response to oxidative stress (Decker and Muller, 2002). Although the role of PARP and caspase-3 in cellular apoptotic signaling pathway have been recognized for some time and gradually known to contribute to lethal effect of chemo-radiotherapy, the exact upstream triggering events leading to activation of this pathway remain unclear (Siegel and McCullough, 2011;Chen et al, 2014). Bcl-2 family members are regulated on the transcriptional level: several pro-survival genes (such as Bcl-2 and Bcl-XL) are induced transcriptionally by certain cytokines, and Bax expression is controlled by several transcription factors including the tumor suppressor proteins P53 and P73 (Adams and Cory, 1998;Muscolini et al, 2008;De et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Bortezomib inhibits gastric carcinoma HGC-27 cells through the phospho-Jun N-terminal kinase (p-JNK) pathway in vitro

Zhang

2015

Gene

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“…The cytochrome c along with apoptotic protease activating factor-1 (Apaf-1), procaspase-9 and ATP could form multiprotein complex (apotosome) in cytoplasm (Zhang, 2007). Then the caspase recruitment domain (CARD) of procaspase-9 and Apaf-1 was activated, and effective caspases, such as caspase-3, caspase-6, and caspase-7, were thus activated (Chen et al, 2014;Licht et al, 2014). Finally, cancer cells apoptosis took place.…”

Section: Discussionmentioning

confidence: 99%

Anticancer effect of 2,7-dihydroxy-3-methylanthraquinone on human gastric cancer SGC-7901 cellsin vitroandin vivo

Zhu

Zheng

Zhang

et al. 2015

Pharmaceutical Biology

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Objective: The objective of this study is to study the anticancer effect and mechanism of DDMN on SGC-7901 cells. Materials and methods: The MTT assay was used to determine the effect of DDMN on cell viability of SGC-7901 cells, and the cytotoxic effect was evaluated by the IC 50 value. After treatment with different doses of DDMN (10, 20, and 40 mM) for 48 h, flow cytometry was used to investigate the apoptosis of SGC-7901 cells induced by DDMN. Further, western blotting was performed to study anticancer mechanism by assaying apoptosis-related proteins containing Mcl-1, Bcl-xl, Bcl-2, Bax, Bak, Bad, cytochrome c, caspase-3, and caspase-9. Finally, xenograft assay was used to further evaluate the effect of DDMN on SGC-7901 cells by determining body weight of nude mice, tumor volumes, and apoptosis-related proteins. Results: These results suggest that DDMN can significantly inhibit (IC 50 value ¼ 20.92 mM) the proliferation of SGC-7901 cells and induce apoptosis of SGC-7901 cells demonstrated by flow cytometry analysis. Additionally, the results of western blotting indicated that DDMN can suppress the expression of anti-apoptotic proteins Bcl-xl and Bcl-2, increase the expression of pro-apoptotic proteins Bax, Bad (40 mM), caspase-3 and caspase-9, and evidently promote the release of cytochrome c from the mitochondria to the cytoplasm. The xenograft assay further confirmed that DDMN had significant anticancer effects on SGC-7901 cells. Conclusion: DDMN had significant anticancer effect on SGC-7901 cells in vitro and in vivo related to mitochondria-mediated apoptosis.

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Cordycepin induces apoptosis of C6 glioma cells through the adenosine 2A receptor-p53-caspase-7-PARP pathway

Cited by 59 publications

References 31 publications

Cordycepin Induces Apoptosis and Inhibits Proliferation of Human Lung Cancer Cell Line H1975 via Inhibiting the Phosphorylation of EGFR

Cordycepin Induces Apoptosis and Inhibits Proliferation of Human Lung Cancer Cell Line H1975 via Inhibiting the Phosphorylation of EGFR

Bortezomib inhibits gastric carcinoma HGC-27 cells through the phospho-Jun N-terminal kinase (p-JNK) pathway in vitro

Anticancer effect of 2,7-dihydroxy-3-methylanthraquinone on human gastric cancer SGC-7901 cellsin vitroandin vivo

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