Core imprint cytology of screen-detected breast lesions is predictive of the histologic results

Abstract

Objective To study antibody‐independent contributions of B cells to inflammatory disease activity, and the immune consequences of B‐cell depletion with rituximab, in patients with multiple sclerosis (MS). Methods B‐Cell effector‐cytokine responses were compared between MS patients and matched controls using a 3‐signal model of activation. The effects of B‐cell depletion on Th1/Th17 CD4 and CD8 T‐cell responses in MS patients were assessed both ex vivo and in vivo, together with pharmacokinetic/pharmacodynamic studies as part of 2 rituximab clinical trials in relapsing–remitting MS. Results B Cells of MS patients exhibited aberrant proinflammatory cytokine responses, including increased lymphotoxin (LT):interleukin‐10 ratios and exaggerated LT and tumor necrosis factor (TNF)‐α secretion, when activated in the context of the pathogen‐associated TLR9‐ligand CpG‐DNA, or the Th1 cytokine interferon‐γ, respectively. B‐Cell depletion, both ex vivo and in vivo, resulted in significantly diminished proinflammatory (Th1 and Th17) responses of both CD4 and CD8 T cells. Soluble products from activated B cells of untreated MS patients reconstituted the diminished T‐cell responses observed following in vivo B‐cell depletion in the same patients, and this effect appeared to be largely mediated by B‐cell LT and TNFα. Interpretation We propose that episodic triggering of abnormal B‐cell cytokine responses mediates ‘bystander activation’ of disease‐relevant proinflammatory T cells, resulting in new relapsing MS disease activity. Our findings point to a plausible mechanism for the long‐recognized association between infections and new MS relapses, and provide novel insights into B‐cell roles in both health and disease, and into mechanisms contributing to therapeutic effects of B‐cell depletion in human autoimmune diseases, including MS. ANN NEUROL 2010;67:452–461

One-day core needle biopsy in a breast clinic: 4 years experience

“…Willems et al calculated a conclusive rate between 60-75% [8]. When compared to CNBplus techniques, all test characteristics (as described in the introduction section) including inconclusive rate are similar and/or better in our study [12,20,21,15,13,19,17,16,18].…”

“…TIC has been associated with a sensitivity ranging from 42 to 98% [15,16]. Specificity has ranged from 44 to 100% [17,18].…”

Modified core wash cytology (CWC), an asset in the diagnostic work-up of breast lesions

“…In studies on CWC the sensitivity and specificity varied from 85% to 89% and 72% to 98%, respectively, while the inadequate rate varied from 7% to 42% [1,2]. Studies on TIC showed variable results as well [3][4][5][6][7][8][9][10].…”

“…The percentage of cases with a CWC diagnosis inadequate was comparable in the clinical and preclinical study (4.0% vs. 6,6%). As we stated in the preclinical study, it is important to note that the quality of the results on CWC are for a major part determined by factors such as the experience of the pathologists, the nature and the quality of the collecting medium, the standardised handling of the material by the technicians, and the cytospin monolayer preparation [3,11,13].…”

Preoperative cytological and histological diagnosis of breast lesions: A critical review

“…[80][81][82][83][84][85][86][87][88][89] Unfortunately, only 2 studies used the final M A N U S C R I P T…”