Background. Office workers often have musculoskeletal disorders (MSDs) such as back pain, neck pain, and shoulder pain. Muscular imbalance is a risk factor associated with MSDs. Lower crossed syndrome (LCS) is also referred to as muscle imbalance of the lower extremity and caused by tightness and weakness of muscles in the lumbopelvic area, which affects the normal movement patterns of sitting position. It leads to low back pain and might affect upper body posture differently.This research investigated the effects of lower crossed syndrome on a craniovertebral angle, sagittal shoulder angle, and trunk flexion angle during sitting in female office workers. Methods. Forty-eight office workers (healthy:18, LCS type A:18, LCS type B:18) who work in computer use for at least 4 hours/day, office work experience at least five years were recruited. Testing posture was a sitting at computer workstation that adjust to each participant. One picture was taken from the lateral view during sitting. One-way ANOVA and post-hoc Turkey test were used to analyze the data. Results. The people with lower crossed syndrome type B have less craniovertebral angles, sagittal shoulder angles, and trunk flexion angle than healthy people and people with lower crossed syndrome type A significantly during sitting. Conclusions. Different types of the lower crossed syndrome affect upper body posture during sitting position differently. The muscle imbalance contributes bad posture which may lead to MSDs.