Core region of <i>Citrobacter</i> lipopolysaccharide from strain PCM 1487

Romanowska, Elżbieta; Gamian, Andrzej; Dąbrowski, Janusz

doi:10.1111/j.1432-1033.1986.tb10478.x

Cited by 32 publications

(12 citation statements)

References 25 publications

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“…The same sugar composition and the same substitution pattern have been previously demonstrated in the LPS core of Citrobacter PCM 1487 [27]. Therefore, the LPS of C. braakii PCM 1531 (O6) and PCM 1487 (O5) may have an identical core region, a suggestion which remains to be proved unambiguously.…”

Section: Elucidation Of the Structure Of The O-specific Polysaccharidsupporting

confidence: 64%

Structural and serological studies on a new 4‐deoxy‐d‐arabino‐hexose‐containing O‐specific polysaccharide from the lipopolysaccharide of Citrobacter braakii PCM 1531 (serogroup O6)

Katzenellenbogen

Kocharova

Zatonsky

et al. 2003

European Journal of Biochemistry

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The O-specific polysaccharide of Citrobacter braakii PCM 1531 (serogroup O6) was isolated by mild acid hydrolysis of the lipopolysaccharide (LPS) and found to contain D-fucose, L-rhamnose, 4-deoxy-D-arabino-hexose and O-acetyl groups in molar ratios 2 : 1 : 1 : 1. On the basis of methylation analysis and 1 H and 13 C NMR spectroscopy data, the structure of the branched tetrasaccharide repeating unit of the O-specific polysaccharide was established. Using various serological assays, it was demonstrated that the LPS of strain PCM 1531 is not related serologically to other known 4-deoxy-D-arabino-hexose-containing LPS from Citrobacter PCM 1487 (serogroup O5) or C. youngae PCM 1488 (serogroup O36). Two other strains of Citrobacter, PCM 1504 and PCM 1505, which, together with strain PCM 1531, have been classified in serogroup O6, were shown to be serologically distinct from strain PCM 1531 and should be reclassified into another serogroup.Keywords: Citrobacter braakii; lipopolysaccharide; O-antigen structure; serological specificity; 4-deoxy-D-arabinohexose.Certain strains of the genus Citrobacter often cause serious opportunistic infections. Most frequently, these bacteria are the aetiological factor of enteric diseases but they are also associated with extraintestinal disorders, among which the most significant are neonatal meningitis and brain abscesses [1,2]. On the basis of genetic studies, the genus Citrobacter has been recently classified into 11 species [3]. Based on their lipopolysaccharides (LPS), strains of Citrobacter are divided into 43 O-serogroups [4] and 20 chemotypes [5].The structures of about 30 different Citrobacter O-antigens (polysaccharide chains of the LPS) have been established and chemical data employed to improve the serological classification of Citrobacter strains and to substantiate multiple cross-reactions between Citrobacter and other genera of the family Enterobacteriaceae, such as Hafnia, Escherichia, Klebsiella and Salmonella [6]. Now we report on the structure of the O-specific polysaccharide (OPS) of Citrobacter braakii PCM 1531, which belongs to serogroup O6 (O6,4b,5b:72) and chemotype VI [5], and on the occurrence in this OPS of 4-deoxy-D-arabino-hexose (ara4dHex). Serological studies were undertaken to determine whether there is any serological relatedness between the LPS of the strain studied and those of Citrobacter strains PCM 1487 and PCM 1488, which also contain the same rarely occurring monosaccharide, as well as of two other Citrobacter strains, PCM 1504 and 1505, which, together with strain PCM 1531, are included in serogroup O6. Materials and methods Gel chromatography and GLC-MSGel-permeation chromatography was carried out on a column (2 · 100 cm) of Sephadex G-50 in 0.05 M pyridinium acetate buffer, pH 5.6, and monitored by the phenol-H 2 SO 4 reaction. GLC-MS was performed with a Hewlett-Packard 5971 instrument (Palo Alto, CA, USA), equipped with an HP-1 glass capillary column (12 m · 0.2 mm), using a temperature program of 150-270°C at 8°CAEmin NMR spectroscopySam...

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Section: Elucidation Of the Structure Of The O-specific Polysaccharidsupporting

confidence: 64%

Structural and serological studies on a new 4‐deoxy‐d‐arabino‐hexose‐containing O‐specific polysaccharide from the lipopolysaccharide of Citrobacter braakii PCM 1531 (serogroup O6)

Katzenellenbogen

Kocharova

Zatonsky

et al. 2003

European Journal of Biochemistry

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“…Weaker but distinct reactions of anti‐conjugate serum were observed with K. pneumoniae , H. alvei , P. shigelloides , S. typhimurium LPSs. Weak or negligible reactions occurred between anti‐conjugate serum and LPS possessing the R3 type of core OS ( E. coli O111, O127, S. flexneri 2b, 3b, 4b) and LPS isolated from C. freundii PCM 1487 with a core OS structure similar to the R3 type [26].…”

Section: Resultsmentioning

confidence: 99%

Serological characterization of anti-endotoxin serum directed against the conjugate of oligosaccharide core ofEscherichia colitype R4 with tetanus toxoid

Łukasiewicz

Jachymek

Niedziela

et al. 2003

FEMS Immunology &Amp; Medical Microbiology

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The covalent conjugate of oligosaccharide core of Escherichia coli type R4 with tetanus toxoid was prepared using reaction of reductive amination. The neoglycoconjugate was a good immunogen in rabbits yielding a high level of anti-lipopolysaccharide (LPS) antibodies of the IgG class. It was found that antiserum was able to react with the smooth LPS molecules of identical (R4) or related (R1) core type. The reactions were shown in the enzyme-linked immunosorbent assay and the immunoblotting test. Flow cytometry showed that anti-core antibodies reacted with LPS present on intact, live, smooth bacteria labelling more than 90% of cells. The anti-OS R4-TT serum used for in vitro studies showed high endotoxin neutralization activity. The serum inhibited endotoxin-induced tumor necrosis factor alpha and nitric oxide synthesis by the J-774A.1 cell line and attenuated pulmonary retention of YAC-1 cells.

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“…The preparation of lipopolysaccharides, Ospecific polysaccharides and core oligosaccharides were performed as previously described [1,2,5].…”

Section: Methodsmentioning

confidence: 99%

“…In the studies on carbohydrate moiety of Citrobacter PCM 1487 strain lipopolysaccharide (LPS) the structures of O-specific polysaccharide (PS) and of core oligosaccharide (OS) were estab-202 lished [1][2][3]. The O-specific polysaccharide consists of the trisaccharide repeating units: The formula of the core oligosaccharide is the following: Rabbit antiserum obtained by immunization with Citrobacter microorganisms is directed against the O-specific polysaccharide chains.…”

Section: Introductionmentioning

confidence: 99%

Immunochemical characterization of Citrobacter strain PCM 1487 O-specific polysaccharide- and core oligossaccharide-protein conjugates

Lugowski¹

1992

FEMS Microbiology Letters

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Core region of Citrobacter lipopolysaccharide from strain PCM 1487

Cited by 32 publications

References 25 publications

Structural and serological studies on a new 4‐deoxy‐d‐arabino‐hexose‐containing O‐specific polysaccharide from the lipopolysaccharide of Citrobacter braakii PCM 1531 (serogroup O6)

Structural and serological studies on a new 4‐deoxy‐d‐arabino‐hexose‐containing O‐specific polysaccharide from the lipopolysaccharide of Citrobacter braakii PCM 1531 (serogroup O6)

Serological characterization of anti-endotoxin serum directed against the conjugate of oligosaccharide core ofEscherichia colitype R4 with tetanus toxoid

Immunochemical characterization of Citrobacter strain PCM 1487 O-specific polysaccharide- and core oligossaccharide-protein conjugates

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