Core-shell Au@PdNPs based colorimetric enhanced lateral flow immunoassay for C-reactive protein detection

Yao, Naizhi; Li, Xiaoting; Tian, Yonghui; Huang, Zhijun; Duan, Yun

doi:10.1016/j.snb.2022.133247

Cited by 14 publications

(6 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The immunosensor parameters were investigated and optimized to enhance its electrochemical performance, which could be affected by the electrolyte pH, incubation time, temperature, and antibody concentration. 27,54,55 As shown in Fig. 3A, the peak currents increased with increasing pH values from 3 to 7, then started to decrease at a pH value greater than 7.…”

Section: Resultsmentioning

confidence: 76%

See 1 more Smart Citation

Ultrasensitive electrochemical immunosensor for the detection of C-reactive protein antigen

Ozoemena,

Boateng,

Chen

2024

Analyst

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 76%

“…Several immunoassays have been developed for the detection of CRP ( e.g. , colorimetric, 23 fluorescence, 24 radioimmunoassay, 25 enzyme, 26 lateral flow 27 ); however, they have some drawbacks ( e.g. , time-consuming, entirely lab-based, etc .…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive electrochemical immunosensor for the detection of C-reactive protein antigen

Ozoemena,

Boateng,

Chen

2024

Analyst

View full text Add to dashboard Cite

show abstract

“…The zeta potential of the AFP antibody and GCA antibody after coupling changed from −18.6 mV to −12.2 mV and −17.2 mV, respectively, indicating the successful synthesis of AgPd@mAb. 34,38…”

Section: Resultsmentioning

confidence: 99%

“…The zeta potential of the AFP antibody and GCA antibody aer coupling changed from −18.6 mV to −12.2 mV and −17.2 mV, respectively, indicating the successful synthesis of AgPd@mAb. 34,38 To obtain the optimal performance of the AgPd@mAb, the pH of the solution and the amount of antibody were optimized, respectively. The pH of the solution was changed by changing the amount of K 2 CO 3 .…”

Section: Optimization Of the Agpd@mabmentioning

confidence: 99%

An AgPd NP-based lateral flow immunoassay for simultaneous detection of glycocholic acid and alpha-fetoprotein

Jiang,

Chen,

Liang

et al. 2024

Anal. Methods

View full text Add to dashboard Cite

show abstract

“…The most commonly used techniques for CRP detection in clinical laboratories include enzyme-linked immunosorbent assays (ELISAs) [ 6 , 7 , 8 ], immunoturbidimetric assays [ 9 , 10 , 11 ], latex immunoagglutination assays [ 12 , 13 ], fluorescence assays [ 14 , 15 , 16 ], chemiluminescence assays [ 17 , 18 ], surface plasmon resonance-based assays [ 19 ], and electrochemical assays [ 20 , 21 , 22 , 23 , 24 , 25 ]. However, these methods are time-consuming and require skilled operators.…”

Section: Introductionmentioning

confidence: 99%

Rapid Microfluidic Immuno-Biosensor Detection System for the Point-of-Care Determination of High-Sensitivity Urinary C-Reactive Protein

Chen,

Lu,

Tseng

et al. 2024

Biosensors

View full text Add to dashboard Cite

A microfluidic immuno-biosensor detection system consisting of a microfluidic spectrum chip and a micro-spectrometer detection device is presented for the rapid point-of-care (POC) detection and quantification of high-sensitivity C-reactive protein (hs-CRP) in urine. The detection process utilizes a highly specific enzyme-linked immunosorbent assay (ELISA) method, in which capture antibodies and detection antibodies are pre-deposited on the substrate of the microchip and used to form an immune complex with the target antigen. Horseradish peroxidase (HRP) is added as a marker enzyme, followed by a colorimetric reaction using 3,3′,5,5′-tetramethylbenzidine (TMB). The absorbance values (a.u.) of the colorimetric reaction compounds are measured using a micro-spectrometer device and used to measure the corresponding hs-CRP concentration according to the pre-established calibration curve. It is shown that the hs-CRP concentration can be determined within 50 min. In addition, the system achieves recovery rates of 93.8–106.2% in blind water samples and 94.5–104.6% in artificial urine. The results showed that the CRP detection results of 41 urine samples from patients with chronic kidney disease (CKD) were highly consistent with the conventional homogeneous particle-enhanced turbidimetric immunoassay (PETIA) method’s detection results (R2 = 0.9910). The experimental results showed its applicability in the detection of CRP in both urine and serum. Overall, the results indicate that the current microfluidic ELISA detection system provides an accurate and reliable method for monitoring the hs-CRP concentration in point-of-care applications.

show abstract

Core-shell Au@PdNPs based colorimetric enhanced lateral flow immunoassay for C-reactive protein detection

Cited by 14 publications

References 30 publications

Ultrasensitive electrochemical immunosensor for the detection of C-reactive protein antigen

Ultrasensitive electrochemical immunosensor for the detection of C-reactive protein antigen

An AgPd NP-based lateral flow immunoassay for simultaneous detection of glycocholic acid and alpha-fetoprotein

Rapid Microfluidic Immuno-Biosensor Detection System for the Point-of-Care Determination of High-Sensitivity Urinary C-Reactive Protein

Contact Info

Product

Resources

About