Core-shell gel wires for the construction of large area heterogeneous structures with biomaterials

Onoe, Hiroaki; Gojo, Riho; Tsuda, Yukiko; Kiriya, Daisuke; Takeuchi, Shoji

doi:10.1109/memsys.2010.5442518

Cited by 13 publications

(10 citation statements)

References 6 publications

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“…Various bottom-up approaches have been developed to overcome the shortcomings associated with the top-down methods such as lack of control over cell distribution in the area or volume of scaffolds. The building blocks in the bottom-up techniques are cell-laden fibers and the assembling methods include random fiber deposition (Sugimoto et al, 2011), weaving (Onoe et al, 2011, Onoe et al, 2010), direct plotting (writing) (Cohen et al, 2006, Fedorovich et al, 2008, Fedorovich, Moroni, 2010, Gaetani, Doevendans, 2012, Ghorbanian, Qasaimeh, 2009), and winding (Liberski et al, 2011). Liberski et al used a core-sheath setup where a core material with high mechanical strength was coated with cell-laden alginate (Liberski, Delaney, 2011).…”

Section: Fiber-based Techniques For Fabricating Cell-laden Constructsmentioning

confidence: 99%

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Fiber-based tissue engineering: Progress, challenges, and opportunities

Tamayol

Akbari

Annabi

et al. 2013

Biotechnology Advances

408

376

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Tissue engineering aims to improve the function of diseased or damaged organs by creating biological substitutes. To fabricate a functional tissue, the engineered construct should mimic the physiological environment including its structural, topographical, and mechanical properties. Moreover, the construct should facilitate nutrients and oxygen diffusion as well as removal of metabolic waste during tissue regeneration. In the last decade, fiber-based techniques such as weaving, knitting, braiding, as well as electrospinning, and direct writing have emerged as promising platforms for making 3D tissue constructs that can address the above mentioned challenges. Here, we critically review the techniques used to form cell-free and cell-laden fibers and to assemble them into scaffolds. We compare their mechanical properties, morphological features and biological activity. We discuss current challenges and future opportunities of fiber-based tissue engineering (FBTE) for use in research and clinical practice.

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Section: Fiber-based Techniques For Fabricating Cell-laden Constructsmentioning

confidence: 99%

“…(a) Schematic of the setup; (b) image of a woven cell fabric formed by fibers made of a core collagen-cell mixture and a calcium alginate sheath (Onoe, Gojo et al 2010). …”

Section: Figurementioning

confidence: 99%

Fiber-based tissue engineering: Progress, challenges, and opportunities

Tamayol

Akbari

Annabi

et al. 2013

Biotechnology Advances

408

376

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“…NSC fibers were produced in a double coaxial laminar flow microfluidic device as a cell-encapsulating core-shell hydrogel fiber as previously reported [6,7]. The core stream of cortical cells suspended (1-2×10 8 cells/ml) in atelocollagen sol and the shell stream of sodium alginate (Na-alginate) sol formed a core-shell laminar co-flow in the device.…”

Section: Nscs Fiber Formationmentioning

confidence: 99%

Specially patterned and aligned neural bundle formed by neural stem cell microfibers

Kato-Negishi

Onoe

Takeuchi

2013

2013 IEEE 26th International Conference on Micro Electro Mechanical Systems (MEMS)

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This paper describes preliminary result of a specially patterned and aligned neural bundle formed by neural stem cell (NSC) microfibers for neural transplantation. The NSC microfibers are made by core-shell hydrogel microfiber encapsulating mouse NSCs. We developed a technique to align microfibers in a straight line and arrange the fibers in special patterns. Using this technique, we formed a neural bundle covered with neural-supporting cells (glial cell fibers). We believe that our neural bundle would be an extremely effective tool for tissue engineering to fabricate macroscopic neural tissue constructs and medical transplantation in neurodegenerative diseases: cerebral infarction, stroke, spinal cord injury and peripheral nerve injury.

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“…The double co-axial microfluidic device was fabricated from two pulled glass capillary tubes assembled in tandem and joined by three-way connectors as previously described [3,4]. Briefly, a glass capillary tube (outer diameter = 1mm, inner diameter = 0.6 mm) was pulled to generate sharp tips with 300 μm diameters.…”

Section: Double Co-axial Microfluidic Device Fabricationmentioning

confidence: 99%

“…We first used a microfluidic co-axial device [3] to encapsulate DFAT cells suspended in various ECM within tubular alginate microfibers to optimize the condition to culture the cells as 3D fiber-shaped micro-constructs. DFAT cell efficiently formed into cell fibers within 1-2 days in all conditions, but only the fibrin/collagen mixture was able to maintain the cell fiber construct for more than 5 days.…”

Section: Introductionmentioning

confidence: 99%

Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells

Hsiao

Okitsu

Onoe

et al. 2013

2013 IEEE 26th International Conference on Micro Electro Mechanical Systems (MEMS)

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This paper describes the self-assembly of "cell springs" using smooth muscle-like cells differentiated from multipotent dedifferentiated fat (DFAT) cells. Using a microfluidic co-axial device, DFAT cells suspended in extracellular matrix (ECM) mixture of fibrin and collagen were encapsulated within tubular alginate microfibers and formed into 3D fiber-shaped micro-constructs. Such DFAT cell fibers were then induced to differentiate into smooth muscle cells (SMCs) under various conditions. Upon differentiation induction, DFAT cell fibers spontaneously contracted into coiled spring structures within 3 days. After 3 weeks of differentiation, the differentiated DFAT cell fibers showed synchronized contraction upon electrical stimulation.

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Core-shell gel wires for the construction of large area heterogeneous structures with biomaterials

Cited by 13 publications

References 6 publications

Fiber-based tissue engineering: Progress, challenges, and opportunities

Fiber-based tissue engineering: Progress, challenges, and opportunities

Specially patterned and aligned neural bundle formed by neural stem cell microfibers

Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells

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