Core transcription restores in vitro inhibition of protein synthesis induced by vaccinia virus

Lobo, Denise S.; Rebello, M. A.; Moussatché, Nissin

doi:10.1016/0167-4781(86)90053-9

Cited by 1 publication

(2 citation statements)

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“…In the presence of transcription inhibitor vaccinia virus produces small polyadenylated transcripts [26]. In addition, the in vitro inhibition of host protein synthesis, induced by vaccinia cores in the absence of viral gene expression [2], does not occur when viral transcription proceeds [7,15]. The latter observation also indicates that viral gene expression is necessary for shutoff of host protein synthesis.…”

Section: Discussionmentioning

confidence: 98%

“…However, using a coupled transcriptiontranslation system we have shown that the inhibition of endogenous protein synthesis does not occur [7,15]. The data presented above mainly reveal that the studies are not complete enough to evaluate if the VV-mediated shutoff requires or not viral gene expression, and whether this inhibitory process depends upon early or/and late viral events.…”

Section: Introductionmentioning

confidence: 93%

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Protein synthesis in vaccinia virus-infected cells

Damaso

Moussatché

1992

Archives of Virology

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Human Hep-2 cells were submitted to hypertonic shock (210 mM NaCl) to block host protein synthesis before infection with vaccinia virus. With the start of infection, the medium isotonicity (116 mM NaCl) was restored, and the effect of viral infection on the recovery of host polyribosomes and protein synthesis was studied. Although host translation blockage was released together with infection, vaccinia virus did not affect immediately host protein synthesis. During the first hour of recovery, infected cells could perfectly rebuild the polyribosome profile and recuperate the rate of protein synthesis. Also, during recovery, formation of the initiation complex for protein synthesis was not affected by viral infection. In this period, viral mRNA and proteins were detected by slot blot and SDS-polyacrylamide gel electrophoresis. The inhibitory effect of vaccinia virus on host translation was observed after the second hour of infection. These findings suggest that vaccinia virus-mediated shutoff occurs in a later period during infection, in parallel with viral mRNA accumulation in the polyribosomes and after the on-set of viral DNA replication.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 93%