CORK1, a LRR-Malectin Receptor Kinase for Cellooligomer Perception in <i>Arabidopsis thaliana</i>

Tseng, Yu-Heng; Scholz, Sandra S.; Fliegmann, Judith; Krüger, Thomas; Gandhi, Akanksha; Kniemeyer, Olaf; Brakhage, Axel A.; Oelmüller, Ralf

doi:10.1101/2022.04.29.490029

Cited by 5 publications

(2 citation statements)

References 104 publications

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“…4). The function of AT1G56145 RLKs as a receptor for cellulose derived oligosaccharides has also been recently proposed by Tseng et al ., (2022; bioRxiv preprint (60)), that identified the Cellulose Oligosaccharide Receptor Kinase 1 (CORK1) in a similar genetic screening to the one performed here; but without reporting any binding of ECD-CORK1 to cellulose-derived oligosaccharides. We now demonstrate that AT1G56145 RLKs is a true PRR receptor for CEL3/CEL5 oligosaccharides further corroborating its function in Arabidopsis thaliana immunity.…”

Section: Discussionmentioning

confidence: 80%

“…We hypothesize that the LRR domain of ECDs in IGP1/AT1G56145 might be essential for the formation of the structural pocket involved in CEL3 and CEL5 binding, since glycoligands for ANX1/ANX2 MAL domains have not been identified (65), though this hypothesis deserve experimental confirmation. In the recent article by Tseng et al , (60), two Phe residues conserved in all Arabidopsis thaliana MAL domains, but not in MAL from Xenopus sp. (53) has been suggested to be essential for CEL3 perception and PTI activation, but this hypothesis has not been validated through binding experiments.…”

Section: Discussionmentioning

confidence: 99%

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Arabidopsis Leucine Rich Repeat-Malectin Receptor Kinases in immunity triggered by cellulose and mixed-linked glucan oligosaccharides

Martín-Dacal

Fernández-Calvo

Jiménez‐Sandoval

et al. 2022

Preprint

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Plant immune system perceives through the extracellular ectodomains (ECDs) of Pattern Recognition Receptors (PRRs) a diversity of carbohydrate ligands from plant and microbial cell walls, which activate Pattern-Triggered Immunity (PTI). Among these ligands are oligosaccharides derived from mixed-linked β-1,3/β-1,4-glucans (MLGs, e.g., β-1,4-D-(Glc)2-β-1,3-D-Glc, MLG43) and cellulose (e.g., β-1,4-D-(Glc)3, CEL3). The mechanisms of perception of carbohydrates by plants are poorly characterized, with the exception of that determining recognition of fungal chitin oligosaccharides (e.g., β-1,4-D(GlcNAc)6, CHI6) that involves several PRRs with LysM-ECDs that function as receptor or co-receptors. Here, we describe the isolation and characterization of Arabidopsis thaliana mutants impaired in glycan perception (igp), which are defective in PTI activation mediated by MLG43 and CEL3, but not CHI6. igp1-igp4 are altered in receptor-like kinases [RLKs: AT1G56145 (IGP1), AT1G56130 (IGP2/3), and AT1G56140 (IGP4)] with Leucine-Rich-Repeat (LRR) and Malectin (MAL) domains in their ECDs. igp4 is a T-DNA insertional, loss of function mutant whereas igp1 and the allelic igp2/igp3 harbour point mutations (E906K and G773E, respectively) in their kinase domains, which impact their structure and surface electrostatic potential as revealed by in silico structural analyses. Notably, Isothermal Titration Calorimetry assays with purified ECD-RLKs showed that AT1G56145 binds with high affinity CEL3 (Kd = 1.19 ± 0.03 μM) and cellopentaose (Kd = 1.40 ± 0.01 μM), but not MLG43, supporting AT1G56145 function as a plant PRR for cellulose oligosaccharides. Our data suggest that these LRR-MAL RLKs are receptor/co-receptors of a novel mechanism of perception of cellulose and MLG-derived oligosaccharides and PTI activation in Arabidopsis thaliana.

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Arabidopsis Leucine Rich Repeat-Malectin Receptor Kinases in immunity triggered by cellulose and mixed-linked glucan oligosaccharides

Martín-Dacal

Fernández-Calvo

Jiménez‐Sandoval

et al. 2022

Preprint

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Arabidopsis LRR-MAL Receptor-like Kinasesregulate intraspecific and interspecific pollen-stigma interactions

Lee,

Sanchez,

Bordeleau

et al. 2023

Preprint

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Flowering plants contain tightly controlled pollen-pistil interactions required for promoting intraspecies fertilization and preventing interspecies hybridizations. In Arabidopsis, several receptor kinases (RKs) are known to regulate the later stages of intraspecies pollen tube growth and ovular reception in the pistil, but less is known about RK regulation of the earlier stages. The Arabidopsis RKF1 cluster of Leucine-Rich Repeat Malectin (LRR-MAL) RKs was previously found to function in the stigma to promote intraspecies pollen hydration. Here, we tested additional combinations of up to seven Arabidopsis LRR-MAL RK knockout mutants for the RKF1 cluster, LIK1, RIR1 and NILR2. These LRR-MAL RKs were discovered to function in the female stigma to support intraspecies Arabidopsis pollen tube growth and to establish a pre-zygotic interspecies barrier against Capsella rubella pollen. Thus this study uncovered new biological functions for these poorly understood group of RKs in regulating the early stages of Arabidopsis sexual reproduction.

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Signals and Their Perception for Remodelling, Adjustment and Repair of the Plant Cell Wall

Oelmüller

Tseng

Gandhi

2023

IJMS

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The integrity of the cell wall is important for plant cells. Mechanical or chemical distortions, tension, pH changes in the apoplast, disturbance of the ion homeostasis, leakage of cell compounds into the apoplastic space or breakdown of cell wall polysaccharides activate cellular responses which often occur via plasma membrane-localized receptors. Breakdown products of the cell wall polysaccharides function as damage-associated molecular patterns and derive from cellulose (cello-oligomers), hemicelluloses (mainly xyloglucans and mixed-linkage glucans as well as glucuronoarabinoglucans in Poaceae) and pectins (oligogalacturonides). In addition, several types of channels participate in mechanosensing and convert physical into chemical signals. To establish a proper response, the cell has to integrate information about apoplastic alterations and disturbance of its wall with cell-internal programs which require modifications in the wall architecture due to growth, differentiation or cell division. We summarize recent progress in pattern recognition receptors for plant-derived oligosaccharides, with a focus on malectin domain-containing receptor kinases and their crosstalk with other perception systems and intracellular signaling events.

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CORK1, a LRR-Malectin Receptor Kinase for Cellooligomer Perception in Arabidopsis thaliana

Cited by 5 publications

References 104 publications

Arabidopsis Leucine Rich Repeat-Malectin Receptor Kinases in immunity triggered by cellulose and mixed-linked glucan oligosaccharides

Arabidopsis Leucine Rich Repeat-Malectin Receptor Kinases in immunity triggered by cellulose and mixed-linked glucan oligosaccharides

Arabidopsis LRR-MAL Receptor-like Kinasesregulate intraspecific and interspecific pollen-stigma interactions

Signals and Their Perception for Remodelling, Adjustment and Repair of the Plant Cell Wall

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