Corn Steep Liquor as a Nutrition Adjunct for the Production of Aspergillus niger Lipase and Hydrolysis of Oils Thereof

Abstract

Corn steep liquor (CSL) has been used as a nutrition adjunct for the production of an extracellular lipase from Aspergillus niger, which has immense importance as an additive in laundry detergent formulations. A five-level four-factorial central composite design was chosen to determine the optimal medium components with four critical variables, namely, CSL, NH4H2PO4, Na2HPO4, and sesame oil, that were found to be influential for lipase production by the classical one-factor-at-a-time method. The model suggested that all of the factors chosen had a significant impact on lipase production, and the optimum values of the influential parameters were CSL, 2.0%, w/v; NH4H2PO4, 0.05%, w/v; Na2HPO4, 0.75%, w/v; and sesame oil, 2.0%, w/v, with an activity of 26.7 U/mL at 48 h and 30 degrees C, which was 2.16-fold higher than the initial activity (12 U/mL) obtained by the conventional one-factor-at-a-time method. Furthermore, the enzyme has good potential for the hydrolysis of vegetable oils and fish oils, and a hydrolytic ratio of 88.73% was obtained with palm oil at 48 h. The utilization of CSL and sesame oil for lipase production from A. niger makes the process green, because both are renewable substrates and economically viable at an industrial scale.

Heterologous expression of lipases YLIP4, YLIP5, YLIP7, YLIP13, and YLIP15 fromYarrowia lipolyticaMSR80 inEscherichia coli: Substrate specificity, kinetic comparison, and enantioselectivity

“…In case of castor oil that is rich in hydroxyl fatty acids, YLIP15 showed an unusually high hydrolysis. This is in contrast to most reports that suggest that generally lipases from fungi, such as Aspergillus niger , Rhizopus delemar , Humicola lanigunose , and bacterial origin are able to hydrolyze castor oil .…”

Green Conversion of Agroindustrial Wastes into Chitin and Chitosan by Rhizopus arrhizus and Cunninghamella elegans Strains

“…Santos et al [10] reported that the increase in biomass production by C. elegans is due to the presence of considerable amounts of amino acids (alanine, arginine, histidine, leucine, lysine, tyrosine, phenylalanine) and vitamins (biotin, choline, inositol, niacin, pyridoxine, thiamine) that are essential for the growth of the microorganism. Edwinoliver et al [29] also suggest that the use of corn steep liquor for lipase production from Aspergillus niger makes the process green, because this substrate is renewable and economically viable on an industrial scale.…”

Optimization of Corn Steep Liquor Dosage and Other Fermentation Parameters for Ethanol Production by Saccharomyces cerevisiae Type 1 and Anchor Instant Yeast

“…It is also a cheap source of proteins, amino acids, minerals, vitamins, and trace elements and can be used as a rich and effective nutritional substitute for expensive complex media like beef extract, meat extract, yeast extract, and peptone in fermentation studies [12][13][14][15]. Studies have shown that CSL can be used as a nutrition supplement and a cheap nitrogen source for cellular growth in the production of some fermentation products [12,14,16]. Edwinoliver et al [14] showed that the utilization of CSL led to a reduction in the overall cost of lipase production medium by 55-60% of the total medium cost, thereby making the process economically attractive.…”

“…Studies have shown that CSL can be used as a nutrition supplement and a cheap nitrogen source for cellular growth in the production of some fermentation products [12,14,16]. Edwinoliver et al [14] showed that the utilization of CSL led to a reduction in the overall cost of lipase production medium by 55-60% of the total medium cost, thereby making the process economically attractive. CSL was also shown to be significant out of the ten low-cost media formulated for Clostridium ragsdalei in ethanol production [17].…”