Corn Steep Liquor: Green Biological Resources for Bioindustry

Zhou, Kemeng; Yu, Jian Qiang; Ma, Yaohong; Cai, Lei; Li, Zheng; Gong, Weili; Liu, Qing-Ai

doi:10.1007/s12010-022-03904-w

Cited by 34 publications

(26 citation statements)

References 100 publications

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“…Although YE favored γ-PGA production, high cost of YE is the main obstacle to the industrial production of γ-PGA. Corn steep liquor is a by-product of the wet milling process and contains crude proteins, amino acids, vitamins, and other nutrients [ 29 ]. It has been employed as an inexpensive source of nitrogen for microorganisms in the production of enzymes, antibiotics, and other fermentation products [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

Efficient molasses utilization for low-molecular-weight poly-γ-glutamic acid production using a novel Bacillus subtilis strain

Chen

et al. 2022

Microb Cell Fact

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Background Poly-γ-glutamic acid (γ-PGA) is a biopolymer and has various applications based on its biocompatibility, non-toxicity, and edibility. Low-molecular-weight (Mw)-γ-PGA has promising applications in agriculture and pharmaceuticals. It is traditionally produced by enzymatic hydrolysis. Cost-effective bioproduction of low-Mw-γ-PGA is essential for commercial application of γ-PGA. Results Bacillus subtilis 242 is a newly isolated low-Mw-γ-PGA-producing strain. To develop cost-effective production of γ-PGA using this newly isolated strain, cane molasses and corn steep liquor were used to produce γ-PGA. The concentration of cane molasses was optimized and 100 g/L cane molasses resulted in high γ-PGA production. The effects of yeast extract and corn steep liquor on γ-PGA yield were investigated. High concentration of γ-PGA was obtained in the medium with corn steep liquor. A concentration of 32.14 g/L γ-PGA was achieved in fed-batch fermentation, with a productivity of 0.67 g/L/h and a percentage yield (gγ-PGA/gglutamate) of 106.39%. The Mw of γ-PGA was 27.99 kDa. Conclusion This study demonstrated the potential application of B. subtilis 242 for cost-effective production of low-Mw-γ-PGA from cane molasses.

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Section: Resultsmentioning

confidence: 99%

Efficient molasses utilization for low-molecular-weight poly-γ-glutamic acid production using a novel Bacillus subtilis strain

Chen

et al. 2022

Microb Cell Fact

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“…Also, the lactic acid yield and productivity also achieved the highest value of 0.89 ± 0.01 g/g and 0.48 ± 0.00 g/L.h, respectively. The previous results described the acidity of CSW and its contents of total sugars, total sulfites, total free amino acids, total reducing sugars, and total nitrogen, showing the value of CSW as a substrate for fermentation [39]. CSW has been utilized as a source of additional nutrients in fermentation processes and growth media [40].…”

Section: Effect Of Different Concentrations Of Ye On La Productionmentioning

confidence: 95%

Statistical optimization of the fermentation factors for increased biorefinery of corn-steep water effluent to lactic acid by Enterococcus faecium strain WH51-1

Selim

Fouda

Salem

et al. 2022

Biomass Conv. Bioref.

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Organic waste bioprocessing for production of useful products are increasing due to fossil fuel depletion, problems with waste management, and the requirement for biodegradable plastics. Lactic acid (LA) is important in foods, polymer beverages, cosmetics, and medicine applications. However, the main challenge in the large-scale processing of LA is the enhanced production and productivity using low-cost resources. In this study, Enterococcus faecium WH51-1, a newly obtained thermo-alkali tolerant LA bacterium, was used for LA production from corn-steep water (CSW) as raw material. The optimal culture conditions and medium components were established. Different concentrations of yeast extract (YE) and pH values were investigated with highest LA production of 48.2 ± 2.7 g/L at a yield of 0.89 ± 0.04 g/g-consumed sugars and a productivity of 0.50 ± 0.03 g/L.h at pH 8.5 without YE supplementation. A central composite design (CCD) was utilized to determine the high LA concentrations at optimal values for the process variables (sugar concentrations, temperature, inocula sizes, pH value, and YE concentration). The model was realized with an acceptable fit. The optimization of the factors was carried out by normal probability plots, interaction-plots, incorporating effect plots, analysis of variance (ANOVA), surface plots, Pareto charts, and contour plots. All the parameters were influenced at a 5% significance level. Additionally, some of the possible interactions between these parameters also influenced the production process. A regression model was suggested and fitted the experimental data very well. The results of this work investigated that LA synthesized of the optimized-fermentation conditions was 10% greater than classical optimization methods. This study presented a green “free nutrient” and cost-effective utilization of corn steep effluent as a cheap alternative substrate for biorefinery to lactic acid. Graphical Abstract

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“…16,17 Corn steep liquor (CSL) is a low-cost substance that can be used in fermentation technology as a precursor, nitrogen source, vitamin, protein and mineral. 18 CSL has been shown in several studies to be an effective replacement for expensive media components such as extract of malt and yeast, and urea in fermentation studies of bioethanol. 18,19 For instance, when compared to yeast extract, CSL can produce nutrient-enhanced ethanol in a short period of the fermentation process.…”

Section: Introductionmentioning

confidence: 99%

“…18 CSL has been shown in several studies to be an effective replacement for expensive media components such as extract of malt and yeast, and urea in fermentation studies of bioethanol. 18,19 For instance, when compared to yeast extract, CSL can produce nutrient-enhanced ethanol in a short period of the fermentation process. 11 Risso et al 20 reported that CSL is a good replacement for vitamin solution in butanol production.…”

Section: Introductionmentioning

confidence: 99%

“…Although numerous studies on the utilization of CSL have been conducted, significant effort has also reportedly been made to increase the output of bioethanol utilizing yeast with high tolerance. 11,18,22,23 Yet there is a continuous effort to establish and model optimal process conditions for the production of bioethanol on a large scale. [24][25][26] In addition, there is a limited amount of literature on modeling studies on bioethanol production that have considered the use of CSL as a nutrient supplement.…”

Section: Introductionmentioning

confidence: 99%

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Comparative evaluation of bioethanol fermentation process parameters using RSM, ANN and ANFIS

Taiwo

Musonge

2023

Biofuels Bioprod Bioref

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The drive for renewable energy as an alternative to fossil fuel is on the increase globally. Modeling a renewable energy process is crucial for increasing process monitoring and control of plant efficiency for the ultimate objective of optimal biorefinery operations. This study aims to develop and compare models that best predict the fermentation process parameters of bioethanol production using corn-steep liquor (CSL) as a media supplement. The response surface method (RSM), artificial neural networks (ANNs) and adaptive neuro-fuzzy inference system (ANFIS) were tested in the modeling of bioethanol fermentation processes. Box-Behnken design was used to investigate fermentation process parameters considering the effect of CSL (0.5-5.5 w/v), pH (4, 5), time (12-36 h), temperature (25-35°C) and inoculum size (0.5-5.5 v/v). The results from the kinetics study show media formulation with corn steep liquor results in a comparable yield with that substituted with yeast extract. The study shows that, for CSL, a maximum ethanol concentration of 17.40 g L −1 was obtained after 48 h of fermentation while 21.53 g L −1 was attained after 6 h for the media formulation with yeast extract. Based on the model evaluation using statistical error indices, ANN predictability was better at R 2 = 0.90; R = 0.95; SEP = 1.73. The ANN models described the process better than ANFIS and RSM. This study shows the intelligent predictive ability of ANN that could be useful for the scale-up process of ethanol production in industry.

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Corn Steep Liquor: Green Biological Resources for Bioindustry

Cited by 34 publications

References 100 publications

Efficient molasses utilization for low-molecular-weight poly-γ-glutamic acid production using a novel Bacillus subtilis strain

Efficient molasses utilization for low-molecular-weight poly-γ-glutamic acid production using a novel Bacillus subtilis strain

Statistical optimization of the fermentation factors for increased biorefinery of corn-steep water effluent to lactic acid by Enterococcus faecium strain WH51-1

Comparative evaluation of bioethanol fermentation process parameters using RSM, ANN and ANFIS

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