Corneal Antifibrotic Switch Identified in Genetic and Pharmacological Deficiency of Vimentin

Bargagna‐Mohan, Paola; Paranthan, Riya R.; Hamza, Adel; Zhan, Chengjun; Lee, Do Min; Kim, Kyung Bo; Lau, Daniel L.; Srinivasan, Cidambi; Nakayama, Keiko; Nakayama, Keiichi I.; Herrmann, Harald; Mohan, Royce

doi:10.1074/jbc.m111.297150

Cited by 39 publications

(90 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, studies are now being carried out to better understand and treat the corneal pathology seen in response to nerve gas treatment (Gordon et al, 2010; McNutt et al, 2012). The most frequently used model to study the corneas response to chemical injury involves soaking circular filters in 0.15M NaOH (Bargagna-Mohan et al, 2012; Lee et al, 2013). Alkali wounds induce a rapid and intense immune response.…”

Section: Variations In Wound Typesmentioning

confidence: 99%

Wounding the cornea to learn how it heals

Stepp

Zieske

Trinkaus‐Randall

et al. 2014

Experimental Eye Research

134

128

View full text Add to dashboard Cite

Corneal wound healing studies have a long history and rich literature that describes the data obtained over the past 70 years using many different species of animals and methods of injury. These studies have lead to reduced suffering and provided clues to treatments that are now helping patients live more productive lives. In spite of the progress made, further research is required since blindness and reduced quality of life due to corneal scarring still happens. The purpose of this review is to summarize what is known about different types of wound and animal models used to study corneal wound healing. The subject of corneal wound healing is broad and includes chemical and mechanical wound models. This review focuses on mechanical injury models involving debridement and keratectomy wounds to reflect the authors’ expertise.

show abstract

Section: Variations In Wound Typesmentioning

confidence: 99%

Wounding the cornea to learn how it heals

Stepp

Zieske

Trinkaus‐Randall

et al. 2014

Experimental Eye Research

134

128

View full text Add to dashboard Cite

show abstract

“…Vimentin filaments in the mesenchymal cells are involved in motility, maintenance of cell shape, and endurance to mechanical stress [16]. In particular, vimentin-deficient mice exhibit delayed wound healing [17]. Therefore, vimentin filaments play a role in wound healing and the development of tissue fibrosis.…”

Section: Introductionmentioning

confidence: 99%

Needle-free jet injection of hyaluronic acid improves skin remodeling in a mouse model

Kwon

Seok

Jang

et al. 2016

European Journal of Pharmaceutics and Biopharmaceutics

View full text Add to dashboard Cite

“…Vimentin is one of the many intermediate filaments of mesenchymal cells such as fibroblasts and endothelial cells (8). Vimentin is needed during tissue repair and its overexpression plays an unappreciated pathogenic role in corneal fibrotic conditions (9). Fibroblasts and myofibroblasts synthesize the ECM components, including collagen types I and III, and fibronectin (10).…”

Section: Introductionmentioning

confidence: 99%

Effects of different sutures on fibrosis and wound healing in a rabbit model of corneal wounds

Liu

Chen

Zhang

et al. 2016

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

The aim of the study was to investigate wound healing and scar formation in rabbit corneal lamellar wounds repaired with simple interrupted sutures (SIS) or horizontal mattress sutures (HMS). Two parallel ‘I’-shaped lamellar cornea wounds were created in one eye of 40 white New Zealand rabbits, while 5 uninjured rabbits were sacrificed to serve as normal controls. One side of the wounds, in the test rabbits, was closed with SIS, while the other side was treated with HMS. Ten days later, the stitches were removed under anesthesia. The animals were sacrificed on days 14 and 21, and months 3 and 6 after the suturing surgery, and corneal samples were subjected to histological and immunofluorescent studies: α-smooth muscle actin (α-SMA) and vimentin were used to detect myofibroblasts and fibroblasts, respectively, and collagen type I and III was used to detect extracellular matrix (ECM) deposition. Relevant mRNA levels were assessed by quantitative polymerase chain reaction (qPCR) to elucidate the differences in wound healing and formation of fibrosis. Macroscopic and hematoxylin and eosin staining observations showed that the two sides of the wounds developed the most prominent fibrotic tissue on day 21. The immunofluorescence and qPCR results showed that HMS wounds produced increased α-SMA, vimentin and collagen type III compared to the SIS wounds on day 14 or 21. The collagen type I expression showed no distinctive difference in SIS and HMS wounds. In conclusion, corneal lamellar wounds treated with SIS developed less fibrotic-related proteins and related mRNA in the early stages of wound healing than wounds treated with HMS. Although differences were not distinct after 3 months, the results of the present study suggest a benefit in choosing SIS over HMS, as at least the initial fibrotic process seems more benign with SIS. Corneal wounds should be carefully sutured, ensuring the tissue is well aligned.

show abstract

Corneal Antifibrotic Switch Identified in Genetic and Pharmacological Deficiency of Vimentin

Cited by 39 publications

References 80 publications

Wounding the cornea to learn how it heals

Wounding the cornea to learn how it heals

Needle-free jet injection of hyaluronic acid improves skin remodeling in a mouse model

Effects of different sutures on fibrosis and wound healing in a rabbit model of corneal wounds

Contact Info

Product

Resources

About