Corneal endothelial cell density and morphology in ophthalmologically healthy young individuals in Japan: An observational study of 16842 eyes

Ono, Takashi; Mori, Yosai; Nejima, Ryohei; Iwasaki, Takuya; Miyai, Takashi

doi:10.1038/s41598-021-97776-5

Cited by 12 publications

(7 citation statements)

References 28 publications

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“…They found that regardless of age, CD in the posterior layer was inversely and significantly correlated with ECD, CV%, and HEX% [18,26]. The strong and negative correlation between age and ECD, which has already been proven in the literature, could explain the aforementioned results [26][27][28]. Similar to their results, Simsek et al found that the posterior CD in patients with Fuchs uveitis syndrome (FUS), either all concentric annuli or the 0-2 mm zone, was strongly and negatively correlated with ECD (r s = -0.789, p < 0.001 in all concentric annuli; r s = -0.545, p = 0.005 in the 0-2 mm zone) and HEX% (r s = -0.616, p = 0.001 in all concentric annuli; r s = -0.589, p = 0.001 in the 0-2 mm zone) [13].…”

Section: Discussionmentioning

confidence: 72%

Corneal densitometry: an innovative method to quantitatively evaluate corneal changes after phacovitrectomy

et al. 2023

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Background To quantitatively investigate corneal changes and the correlation between corneal densitometry (CD) and endothelial parameters after phacovitrectomy. Methods Thirty-eight eyes with idiopathic full-thickness macular holes (iFTMHs) and cataracts underwent phacovitrectomy. Examinations were conducted at baseline and Day 1, Day 7, Month 1, and Month 3 postoperatively. CD and central corneal thickness (CCT) were measured using Pentacam. Corneal endothelial cell density (ECD), coefficient of variation (CV), and hexagonality (HEX) were measured using specular microscopy. Results ECD and HEX significantly decreased after surgery and the change in HEX occurred prior to CV. CCT increased immediately after surgery and recovered 3 months postoperatively. CD values increased significantly 1 day after surgery and then gradually decreased. For CD in the 0–2 mm zone, it took 1 month to recover in the central and posterior layers and 3 months in the anterior and total layers. For CD in the 2–6 mm zone, the central layer recovered at Day 7, the anterior and total layers recovered at 1 month, and the posterior layer did not recover until 3 months postoperatively. The CD within all layers in the 0–2 mm zone was positively correlated with CCT. Posterior CD in the 0–2 mm zone was negatively correlated with ECD and HEX. Conclusions CD is not only correlated with CCT, ECD, and HEX but also reflects the state of the whole cornea and each layer. CD can be an objective, rapid, and noninvasive tool that reflects corneal health and undetectable edema and monitors the process of lesion repair. Trial registration This study was registered with the Chinese Clinical Trial Registry (31/10/2021, ChiCTR2100052554).

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Section: Discussionmentioning

confidence: 72%

Corneal densitometry: an innovative method to quantitatively evaluate corneal changes after phacovitrectomy

et al. 2023

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“…Figure 1(a) shows a schematic to highlight the range of p 6 values obtained from experiment. The data are subdivided into those obtained as a function of patient age [16][17][18][19][20][21][22][23][24][25][26] , contact lens usage [32][33][34][35] , onset of diabetes [27][28][29][30] , and "other" (here, covering smoking behaviour, non-human species (here, dogs, rabbits and mice) and the behaviour of transplanted tissue 22,31,[57][58][59][60][61] ). A large spread of p 6 values are obtained, ranging from p 6 ∼ 0.36 − 0.88.…”

Section: Trends In P6mentioning

confidence: 99%

“…Experimental data obtained as a function of patient age are shown in figure 2(a). The data are subdivided by the nation of origin of the patient with the published standard deviations shown as error bars [16][17][18][19][20][21][22]25,62,63 . In the majority of cases the fraction of six-membered rings decreases as a function of time (and this is the over-arching trend in all cases).…”

Section: Trends In P6mentioning

confidence: 99%

“…Figure 5(a) shows the variation of the cell area coefficient of variation, CV , with human patient age (data from refs. [16][17][18][19][20][21][22][23]25,26,67,68 ). CV rises gradually, and near-linearly, with age with mean gradient d(CV )/dt ∼ 0.001year −1 .…”

Section: Cell Areasmentioning

confidence: 99%

“…In addition, the areas of the rings can be measured and their deviation from ideal regular polygons can be quantified, for example, by determining the coefficient of variation of the area. Experimental evidence suggests that the structure of the corneal endothelium is affected not only by aging [16][17][18][19][20][21][22][23][24][25][26] , but by other factors such as the onset of diabetes [27][28][29][30] , smoking status 31 or the prolonged wearing of contact lenses [32][33][34][35] . The most common network properties which are tracked experimentally are the cell density, the distribution of ring sizes (cellular polymegethism) and the distribution of the areas of those rings, in particular distortions away from their ideal (symmetric) shapes (cellular pleomorphism).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The network structure of the corneal endothelium

Xuan

Whitaker

Wilson

2023

The Journal of Chemical Physics

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A generic network model is applied to study the structure of the mammalian corneal endothelium. The model has been shown to reproduce the network properties of a wide range of systems, from low-dimensional inorganic glasses to colloidal nanoparticles deposited on a surface. Available extensive experimental microscopy results are analysed and combined to highlight the behaviour of two key metrics, the fraction of hexagonal rings ($p_6$) and the coefficient of variation of the area (CV). Their behaviour is analysed as a function of patient age, the onset of diabetes and contact lens wearing status. Wearing contact lenses for $\sim$10years is shown to change the endothelium structure by the equivalent of $\sim$30years contact lens-free. Model network configurations are obtained using a Monte Carlo bond switching algorithm with the resulting topologies controlled by two potential model parameters (the bond and angular force constants) and the Monte Carlo temperature. The effect of systematically varying these parameters is investigated. In addition, the effect of constraining the ring size distribution is investigated. The networks generated with relatively weak bond force constants are shown to correlate best with the experimental information. The importance of extracting the full ring size distribution (rather than simply the fraction of hexagons) is discussed.

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Multi‐Functional Gelatin‐Dithiolane Hydrogels for Tissue Engineering

Asim,

Tuftee,

Qureshi

et al. 2024

Adv Funct Materials

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Biomaterials that integrate multiple functionalities, mimic the extracellular matrix (ECM) microenvironment to support cellular growth, and adhere robustly to damaged tissues are highly needed to advance tissue engineering. Protein‐based biomaterials are promising due to their inherent biocompatibility, biomimicry, biodegradation, and cell‐supportive properties. Herein, by leveraging the unique ability of dithiolanes to generate on‐demand in situ thiols, a new class of dithiolane‐modified, protein‐based biomaterial that combines unique, seemingly opposing functions for tissue engineering is developed. Dithiolane‐modified gelatin, a model protein used herein, enabled photoinitiator‐free photo‐crosslinking to form multi‐functional gelatin‐dithiolane (GelDT) hydrogels, which displayed exceptional long‐term stability in cell culture media (>28 days) to support the growth of both surface‐seeded and encapsulated cells. GelDT hydrogels allowed pre‐gelation tuning of biomechanical properties and biodegradation via introducing physical crosslinks, and post‐gelation tuning of matrix stress‐relaxation rate, via responding to exogenous thiols, independently of other parameters. Furthermore, GelDT enabled covalent immobilization of bio‐active molecules, glutathione‐responsive drug release, supported efficient 3D bioprinting due to its shear‐thinning ability, and demonstrated robust tissue adhesion in various contexts (bare skin, ex‐vivo, in‐vivo) due to covalent disulfide coupling with endogenous tissue thiols. Together, this study presents a novel multi‐responsive and multi‐functional protein‐based biomaterial, anticipated to advance tissue engineering and regeneration.

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Corneal endothelial cell density and morphology in ophthalmologically healthy young individuals in Japan: An observational study of 16842 eyes

Cited by 12 publications

References 28 publications

Corneal densitometry: an innovative method to quantitatively evaluate corneal changes after phacovitrectomy

Corneal densitometry: an innovative method to quantitatively evaluate corneal changes after phacovitrectomy

The network structure of the corneal endothelium

Multi‐Functional Gelatin‐Dithiolane Hydrogels for Tissue Engineering

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