Corneal Epithelial Wound Healing and Bactericidal Effect of Conditioned Medium From Human Uterine Cervical Stem Cells

Bermúdez, María A.; Sendón-Lago, Juan; Eiró, Noemí; Treviño, Mercedes; González, Francisco; Yebra‐Pimentel, Eva; Giráldez, María Jesús; Macı́a, Manuel; Lamelas, Marı́a L.; Saá, Jorge; Vizoso, F; Perez-Fernandez, Román

doi:10.1167/iovs.14-15859

Cited by 84 publications

(69 citation statements)

References 48 publications

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“…Some studies described the presence of growth factors in the MSCs secretome that promoted tissue regeneration with a special focus on proliferation (Lee et al 2011;Zagoura et al 2012;Turner et al 2013;Bhang et al 2014;Bermudez et al 2015). The experimental myocardial infarction models showed that anti-fibrotic and angiogenic effects of the MSCs secretome inhibited scar formation process (Cargonini et al 2012;Preda et al 2014) and stimulated the synthesis and consequent secretion of bioactive molecules responsible for remodelling (Williams et al 2013;Vizoso et al 2017).…”

Section: Cutaneous Wound Healingmentioning

confidence: 99%

“…This new frontier of research provides several key advantages over cell based applications: (a) the administration of proteins instead of whole cells as a new therapeutic option in regenerative medicine; (b) CM can be stored without any toxic cryopreservatives, such as dimethyl sulfoxide (DMSO), for a relatively long period; (c) preparation of CM is more economical as it can be mass-produced from the available MSC populations under current good manufacturing practice (cGMP) conditions; (d) evaluation of CM for safety and efficacy will be much simpler and analogous to conventional pharmaceutical agents (Bermudez et al 2015;L et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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Biological activity of mesenchymal stem cells secretome as a basis for cell-free therapeutic approach

Pokrovskaya¹,

Zubareva²,

Nadezhdin³

et al. 2020

RRP

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Mesenchymal stem (stromal) cells (MSCs) are self-renewing, cultured adult stem cells which secrete a complex set of multiple soluble biologically active molecules such as chemokines, and cytokines, cell adhesion molecules, lipid mediators, interleukins (IL), growth factors (GFs), hormones, micro RNAs (miRNAs), long non-coding RNAs (lncRNAs), messenger RNAs (mRNAs), exosomes, as well as microvesicles, the secretome. MSCs of various origin, including adipose-derived stem cells (ASCs), bone marrow derived mesenchymal stem cells (BM-MSCs), human uterine cervical stem cells (hUCESCs), may be good candidates for obtaining secretome-derived products. Different population of MSCs can secret different factors which could have anti-inflammatory, anti-apoptotic, anti-fibrotic activities, a neuroprotective effect, could improve bone, muscle, liver regeneration and wound healing. Therefore, the paracrine activity of conditioned medium obtained when cultivating MSCs, due to a plethora of bioactive factors, was assumed to have the most prominent cell-free therapeutic impact and can serve as a better option in the field of regenerative medicine in future.

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Section: Cutaneous Wound Healingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biological activity of mesenchymal stem cells secretome as a basis for cell-free therapeutic approach

Pokrovskaya¹,

Zubareva²,

Nadezhdin³

et al. 2020

RRP

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show abstract

“…Extraction of the secretome from conditioned medium is more economical and practical than large-scale production and administration of stem cells [65]. Application of the secretome removes issues of immune compatibility, tumorigenicity, and infection transmission; it can be assessed similarly to current pharmaceutical agents; long-term storage without diminishing the potency can be achieved without common toxic cryopreservation agents, such as dimethyl sulfoxide (DMSO) [66][67][68]; and manufacturing schedules are significantly reduced in comparison to cell therapies. However, due to a cell's response to signalling affecting the composition of the secretome, producing an efficacious output is challenging [64,69].…”

Section: Innate Kidney Repair and The Influence Of Stem Cellsmentioning

confidence: 99%

A critical review of current progress in 3D kidney biomanufacturing: advances, challenges, and recommendations

2019

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The widening gap between organ availability and need is resulting in a worldwide crisis, particularly concerning kidney transplantation. Regenerative medicine options are becoming increasingly advanced and are taking advantage of progress in novel manufacturing techniques, including 3D bioprinting, to deliver potentially viable alternatives. Cell-integrated and wearable artificial kidneys aim to create convenient and efficient systems of filtration and restore elements of immunoregulatory function. Whilst preliminary clinical trials demonstrated promise, manufacturing and trial design issues and identification of suitable and sustainable cell sources have shown that more development is required for market progression. Tissue engineering and advances in biomanufacturing techniques offer potential solutions for organ shortages; however, due to the complex kidney structure, previous attempts have fallen short. With the recent development and progression of 3D bioprinting, cell positioning and resolution of material deposition in organ manufacture have never seen greater control. Cell sources for constructing kidney building blocks and populating both biologic and artificial scaffolds and matrices have been identified, but in vitro culturing and/or differentiation, in addition to maintaining phenotype and viability during and after lengthy and immature manufacturing processes, presents additional problems. For all techniques, significant process barriers, clinical pathway identification for translation of models to humans, scaffold material availability, and long-term biocompatibility need to be addressed prior to clinical realisation.

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“…Many reporters [25,33,34] clarified that alkali burns induced ulcers are accompanied with the existence of inflammatory cells infiltration in the corneal stroma, which are pointing to the inflammation. They lead to damage of the normal corneal architecture due to liberation of Matrix Metalloproteinases (MMPs) and several proteolytic enzymes that dissolve its collagen fibers.…”

Section: The Light and Electron Microscopic Examination Of Corneal Sementioning

confidence: 99%

Comparative Light and Electron Microscopic Study on the Therapeutic Efficacy of Adipose Derived Stem Cells Versus Exosomes for Experimentally Induced Acute Corneal Injuries in Rats

El-Mahalaway¹,

El-Azab²,

Abdrabbo³

et al. 2018

J Stem Cell Res Ther

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Background: Corneal alkali injuries are considered a common ophthalmologic emergency with many serious complications. Adipose Tissue-Derived Stem Cells (ADSCs) and Mesenchymal Stem Cells derived Exosomes (MSCs-EX) are promising approaches in regenerative therapies. Objective: The aim of this study is to evaluate the efficacy of ADSCs versus MSCs-EX on experimentally alkaliinduced corneal injuries in rats. Materials and Methods: 40 adult male albino rats were divided into four groups, that is, Group I: control rats, Group II: alkali burn rats, Group III: ADSCs treated rats after corneal alkali burn, Group IV: MSCs-EX treated rats after corneal alkali burn. Cornea specimens were taken and processed for histological examination. Results: Group II showed obvious changes such as erosion with focal loss of some superficial epithelial cells, while the basal and intermediate epithelial cell layers had vacuolated cytoplasm with pyknotic nuclei. The substantia propria contained irregularly arranged collagen fibers with wide spacing, keratocytes, and congested blood vessels. Descemet's membrane appeared thin and degenerated with focal loss of some endothelial cells. Ultrastructural examination showed degenerated apical squamous cells with loss of microvilli on their free surface. The basal cells had irregular contour with widening of intracellular space between cells with multiple cytoplasmic vacuoles. The substantia propria showed disorganized collagen fibers with degenerated keratocytes and mononuclear cellular infiltration. Groups III and IV showed improvement of the histological and electron microscopic changes described in group II. Conclusion: ADSCs and MSCs-EX can improve corneal alkali burn injuries and prevent their complications. Exosomes are a more promising therapeutic approach.

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Corneal Epithelial Wound Healing and Bactericidal Effect of Conditioned Medium From Human Uterine Cervical Stem Cells

Abstract: Treatment with CM-hUCESCs improved wound healing of alkali-injured corneas and showed a strong bactericidal effect on CLs. Patients using CLs and suffering from dry eye, allergies induced by commercial solutions, or small corneal injuries could benefit from this treatment.

Cited by 84 publications

References 48 publications

Biological activity of mesenchymal stem cells secretome as a basis for cell-free therapeutic approach

Biological activity of mesenchymal stem cells secretome as a basis for cell-free therapeutic approach

A critical review of current progress in 3D kidney biomanufacturing: advances, challenges, and recommendations

Comparative Light and Electron Microscopic Study on the Therapeutic Efficacy of Adipose Derived Stem Cells Versus Exosomes for Experimentally Induced Acute Corneal Injuries in Rats

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