Corneal Histology After Epidemic Keratoconjunctivitis

Lund, O. E.; Stefani, F. H.

doi:10.1001/archopht.1978.03910060465016

Cited by 51 publications

(31 citation statements)

References 12 publications

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“…standing. 31,32 The pathogenesis and formation of adenoviral subepithelial infiltrates has not been adequately investigated in vivo because of the lack of a suitable animal model.…”

Section: Discussionmentioning

confidence: 43%

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Adenovirus Type 37 Keratitis in the C57BL/6J Mouse

Chintakuntlawar

Astley

Chodosh

2007

Invest. Ophthalmol. Vis. Sci.

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PURPOSE.To develop a mouse model of adenoviral keratitis that will allow further study of viral and host pathogenic mechanisms. METHODS. Corneas of C57BL/6J mice were injected with adenovirus type 37 (Ad37) or virus-free dialysis buffer by a gaspowered microinjection system coupled to a glass micropipette needle. Mouse corneas were examined for signs of inflammation, by clinical examination, immunohistochemistry, and confocal microscopy; assayed for viral and chemokine mRNA expression by real-time PCR; titered to assess viral replication; and subjected to ELISA for chemokine and myeloperoxidase (MPO) protein expression. RESULTS. C57BL/6J mice corneas injected with 10 5 TCID (tissue culture infective dose) Ad37 showed stromal opacification and inflammation beginning from 1 day after injection and continuing for several months, while buffer-injected corneas showed no signs of inflammation. Ad37-injected corneas expressed adenoviral E1A 10S and E1B 19k mRNA but not IIIa, and viral titers had fallen two logs by day 4 after injection. When compared to untouched and buffer-injected corneas, Ad37-injected corneas expressed significantly higher levels of IL-6, KC, and MCP-1 mRNA at 4 hours after injection (P Ͻ 0.05). By ELISA, KC protein was significantly elevated in Ad37-injected corneas at 8 and 16 hours, and MCP-1 protein at 16 hours after injection (P Ͻ 0.05). Ad37-injected corneas showed elevated levels of MPO (P ϭ 0.0024) at 4 days after injection consistent with immunohistochemical evidence for a predominance of neutrophils in the corneal stroma. CONCLUSIONS. Ad37 induces an acute immunopathologic response in the C57BL/6J mouse cornea, despite an absence of viral replication. This new animal model of Ad37 keratitis will facilitate studies of the molecular pathogenesis of the disorder. (Invest Ophthalmol Vis Sci. 2007;48:781-788)

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“…standing. 31,32 The pathogenesis and formation of adenoviral subepithelial infiltrates has not been adequately investigated in vivo because of the lack of a suitable animal model.…”

Section: Discussionmentioning

confidence: 43%

“…31,32 In our study, Ad37 entry and early gene expression were shown to occur in corneal cells, but not viral replication. The absence of adenoviral replication was not surprising considering the relative nonpermissiveness of mouse cells to adenovirus infection seen by others both in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 47%

Adenovirus Type 37 Keratitis in the C57BL/6J Mouse

Chintakuntlawar

Astley

Chodosh

2007

Invest. Ophthalmol. Vis. Sci.

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“…The corneal replicas concerned only the superficial epithelial layers and couldn't therefore exclude a possible presence of leukocytes in the deeper ones. Lymphocytic infiltration of the superficial corneal stroma and the overlying epithelium, however, has been reported in corneal subepithelial infiltrates (Lund & Stefani 1978), a sequel of the infection considered to represent an immune phenomenon (Jones 1962). The present photographs concerned the whole epithelial thickness, and the superficial stroma, but the nature of the abnormal cells couldn't be decided: incipient cell swelling, dying cells with altered membranes (cf.…”

Section: Discussionmentioning

confidence: 45%

Corneal epithelial changes due to adenovirus type 8 infection

Tabery

2000

Acta Ophthalmologica Scandinavica

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“…3 Histopatolojik çalışmalarda, viral keratokonjonktivitler sonrası lenfosit ve fibroblast birikimlerinin ön yüzeyel stroma, Bowman tabakası ve epitelde olduğu gösterilmiştir. 6 Viral keratokonjonktivitlere bağlı oluşan subepitelyal infiltratların tedavisinde topikal steroid, siklosporin ve takrolimus tedavileri uygulanabilmektedir, ancak yanıt-sız veya yetersiz yanıt alınan hastalarda PTK başvurulabilmektedir. 4,5 Özellikle bir yıldan daha uzun süredir mevcut olan subepitelyal bölgedeki infiltratların tedavisinde PTK işleminin etkililiği gösterilmiştir.…”

Section: Cerrahi̇ Prosedürunclassified

“…6 PTK sonrası oluşan hipermetropik kaymanın stromal ablasyon derinliği ile ilişkili olduğu yapılan çalış-malarda gösterilmiştir. 10,11 Çalışmamızdaki stromal ablasyon derinliğinin diğer çalışmalara göre daha az olması, hipermetropik kaymanın daha az olmasına bağlı olabilir.…”

Section: Cerrahi̇ Prosedürunclassified

Phototherapeutic Keratectomy in Treatment of Viral Keratoconjunctivitis Related Corneal Opacities

Yıldırım¹,

Alagöz²,

Garip³

et al. 2016

Turkiye Klinikleri J Ophthalmol

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Ö ÖZ ZE ET T A Am ma aç ç: : Viral keratokonjonktivite bağlı oluşan yüzeyel kornea opasitelerinin tedavisinde fototerapötik keratektominin [phototerapeutic keratectomy (PTK)] uzun dönem sonuçlarının değer-lendirilmesidir. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Viral keratokonjonktivit sekeli yüzeyel kornea opasitelerinin tedavisinde PTK uygulanmış 21 hastanın 32 gözü geriye dönük değerlendirildi. Tüm hastaların görme keskinlikleri, refraktif durumları ve korneal yüksek sıralı aberasyonları PTK öncesinde ve sonrasında değerlendirildi. PTK öncesi ve sonrasındaki 12 ve 24. aylarda tüm hastalara ön segment optik koherens tomografi çekildi ve korneal topografik inceleme yapıldı. Korneal yüksek sıralı aberasyonlar 3 mm'lik santral zonda kombine Scheimpflug-Placido disk topografi cihazı ile değerlen-dirildi. B Bu ul lg gu ul la ar r: : Hastaların ortalama takip süresi 26,4±4,0 (23-32) ay ve ortalama yaşı 24,2±8,4 yıl idi. Görme keskinliğindeki bozulmanın süresi ortalama 13,2±4,2 (12-18) ay idi. Düzeltilmiş ve dü-zeltilmemiş görme keskinliklerinde PTK sonrası 12 ve 24. aylarda anlamlı artış saptandı. İşlem sonrası hipermetropik kayma 12. ayda 0,62±0,28 dioptri iken, 24. ayda 0,58±0,35 dioptri bulundu. Toplam korneal yüksek sıralı, koma, trefoil ve sferik aberasyonlarda anlamlı azalma saptandı. PTK sonrası iki hastanın üç gözünde uzayan epitel defekti (>4 gün) saptandı ve uygun medikal tedavi ile iyileşme sağlandı. S So on nu uç ç: : PTK viral keratokonjoktivit sekeli yüzeyel kornea opasitelerin tedavisinde uzun dönemde etkin ve güvenilirdir.A An na ah ht ta ar r K Ke el li im me el le er r: : Keratokonjonktivit; kornea; tomografi, optik koherens; keratektomi, subepitelyal, lazer yardımlı A AB BS ST TR RA AC CT T O Ob bj je ec ct ti iv ve e: : To report the long term results of phototherapeutic keratectomy (PTK) for the treatment of superficial corneal opacities after epidemic keratokonjunctivitis. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : Thirty-two eyes of 21 patients who were treated with PTK for the superficial corneal opacities induced by epidemic keratoconjunctivitis were retrospectively analyzed. Visual acuity, manifest refraction and high order aberrations were identified for all patients preoperatively and postoperatively. Corneal topography and anterior segment optical coherence tomography were also evaluated preoperatively and at 12 and 24 months postoperatively. High order corneal aberrations were assessed with the use of a Scheimpflug camera combined Placido disc topography in the central 3-mm corneal area. R Re es su ul lt ts s: : The mean follow-up period was 26.4±4.0 (23-32) months and the mean age was 24.2±8.4 years. The patients were suffering from the impaired vision related to corneal opacities for 13.2±4.2 (12-18) months. The uncorrected and corrected distance visual acuities were improved at 12 and 24 months postoperatively. The mean postoperative hypermetropic shifts were 0.62±0.28 and 0.58±0,35 diopters at 12 and 24 months, respectively. The total higher order, com...

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Corneal Histology After Epidemic Keratoconjunctivitis

Cited by 51 publications

References 12 publications

Adenovirus Type 37 Keratitis in the C57BL/6J Mouse

Adenovirus Type 37 Keratitis in the C57BL/6J Mouse

Corneal epithelial changes due to adenovirus type 8 infection

Phototherapeutic Keratectomy in Treatment of Viral Keratoconjunctivitis Related Corneal Opacities

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