Corneal Stem Cells and Their Origins: Significance in Developmental Biology

Davies, Shakiela Khanam; Girolamo, Nick Di

doi:10.1089/scd.2010.0201

Cited by 20 publications

(15 citation statements)

References 114 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the mammalian cornea, epithelial cells with strong proliferative capacity and stem cell-like properties are initially distributed across the entire cornea (Chung et al, 1992;Collinson et al, 2002;Davies et al, 2009). As the embryo develops, corneal stem cells become restricted to the peripheral edge of the cornea, the limbus (Davies and Di Girolamo, 2010). This shift in corneal stem cell location is reflected in the clonal composition of the cornea during the course of development.…”

Section: Long-term Clonal Analyses In the Corneamentioning

confidence: 99%

“…In the cornea, it has long been believed that epithelial stem cells are located exclusively in the limbus and that corneal clones are formed by centripetal growth from the limbus (Davies and Di Girolamo, 2010;Lavker et al, 2004). Interestingly, new evidence suggest that corneal stem cells might be scattered over the entire cornea and that corneal clones may be formed by centrifugal, rather than centripetal, growth (Majo et al, 2008).…”

Section: Clonal Analysesmentioning

confidence: 99%

See 1 more Smart Citation

Zebrabow: multispectral cell labeling for cell tracing and lineage analysis in zebrafish

et al. 2013

View full text Add to dashboard Cite

SUMMARYAdvances in imaging and cell-labeling techniques have greatly enhanced our understanding of developmental and neurobiological processes. Among vertebrates, zebrafish is uniquely suited for in vivo imaging owing to its small size and optical translucency. However, distinguishing and following cells over extended time periods remains difficult. Previous studies have demonstrated that Cre recombinase-mediated recombination can lead to combinatorial expression of spectrally distinct fluorescent proteins (RFP, YFP and CFP) in neighboring cells, creating a 'Brainbow' of colors. The random combination of fluorescent proteins provides a way to distinguish adjacent cells, visualize cellular interactions and perform lineage analyses. Here, we describe Zebrabow (Zebrafish Brainbow) tools for in vivo multicolor imaging in zebrafish. First, we show that the broadly expressed ubi:Zebrabow line provides diverse color profiles that can be optimized by modulating Cre activity. Second, we find that colors are inherited equally among daughter cells and remain stable throughout embryonic and larval stages. Third, we show that UAS:Zebrabow lines can be used in combination with Gal4 to generate broad or tissue-specific expression patterns and facilitate tracing of axonal processes. Fourth, we demonstrate that Zebrabow can be used for long-term lineage analysis. Using the cornea as a model system, we provide evidence that embryonic corneal epithelial clones are replaced by large, wedge-shaped clones formed by centripetal expansion of cells from the peripheral cornea. The Zebrabow tool set presented here provides a resource for next-generation color-based anatomical and lineage analyses in zebrafish.

show abstract

Section: Long-term Clonal Analyses In the Corneamentioning

confidence: 99%

Section: Clonal Analysesmentioning

confidence: 99%

Zebrabow: multispectral cell labeling for cell tracing and lineage analysis in zebrafish

et al. 2013

View full text Add to dashboard Cite

show abstract

“…This is somewhat peculiar in evolutionary sense especially when the directly adjacent conjunctiva is maintained in the same way as any other epithelia [134] . In fetal eyes, adult LSC markers are found in the basal layer across the cornea [135,136] and it is unknown how the markers become segregated in the limbus during development. Investigation of limbal organogenesis has raised a possibility that the limbal papillary structures are mere developmental remnants.…”

Section: Developmental Origin Of Limbusmentioning

confidence: 99%

“…Investigation of limbal organogenesis has raised a possibility that the limbal papillary structures are mere developmental remnants. The limbus does not develop until eyelids open and the ocular surface is exposed to amniotic fluid [135,136] . The papillary structures of the limbus do not form until post-natal life [137] .…”

Section: Developmental Origin Of Limbusmentioning

confidence: 99%

Limbal stem cells: Central concepts of corneal epithelial homeostasis

Yoon¹

2014

WJSC

103

View full text Add to dashboard Cite

A strong cohort of evidence exists that supports the localisation of corneal stem cells at the limbus. The distinguishing characteristics of limbal cells as stem cells include slow cycling properties, high proliferative potential when required, clonogenicity, absence of differentiation marker expression coupled with positive expression of progenitor markers, multipotency, centripetal migration, requirement for a distinct niche environment and the ability of transplanted limbal cells to regenerate the entire corneal epithelium. The existence of limbal stem cells supports the prevailing theory of corneal homeostasis, known as the XYZ hypothesis where X represents proliferation and stratification of limbal basal cells, Y centripetal migration of basal cells and Z desquamation of superficial cells. To maintain the mass of cornea, the sum of X and Y must equal Z and very elegant cell tracking experiments provide strong evidence in support of this theory. However, several recent studies have suggested the existence of oligopotent stem cells capable of corneal maintenance outside of the limbus. This review presents a summary of data which led to the current concepts of corneal epithelial homeostasis and discusses areas of controversy surrounding the existence of a secondary stem cell reservoir on the corneal surface

show abstract

“…Several recent studies showed that under appropriate culture conditions, ESCs can differentiate into corneal epithelial cell (CEC)-like cells, [24][25][26][27] which strongly expressed specific terminally differentiated CEC markers CK3, CK12, and Cx43/50. However, the proliferative capacity of these CEC-like cells was limited and could hardly subculture in vitro.…”

Section: Introductionmentioning

confidence: 99%

Reconstruction of Functional Ocular Surface by Acellular Porcine Cornea Matrix Scaffold and Limbal Stem Cells Derived from Human Embryonic Stem Cells

Zhu

Zhang

Sun

et al. 2013

Tissue Engineering Part A

View full text Add to dashboard Cite

Limbal stem cells (LSCs) derived from human embryonic stem cells (hESCs) hold great potential for cell-based therapies for ocular surface diseases. The cell-based therapies mainly depend on an appropriate differentiation proposal with a high efficiency and on a suitable scaffold. In this study, we aimed to establish a feasible and efficient strategy for inducing hESCs into LSC-like cells by the LSC conditioned medium. The induced cells possessed the similar morphologic characteristics and expression of normal LSCs and showed a strong clonogenic and proliferative capacity in vitro. To construct a tissue-engineering corneal graft, these differentiated cells were seeded on an acelluar porcine corneal matrix (APCM), which maintained the corneal basement membrane in vitro. After 14 days culture, these induced cells gave rise to stratified epithelial cell sheets on the APCM and the basal cells still kept LSC characteristics. In rabbit total limbal stem cell deficiency (LSCD) models, the tissue-engineering graft had the potential to reconstruct the damaged ocular surface and alleviated the invasion of corneal neovascularization. These findings indicated that the engraftment constructed with the APCM scaffold and hESC-derived LSCs might be a potential therapy option for ocular surface regeneration in LSCD cases.

show abstract

Corneal Stem Cells and Their Origins: Significance in Developmental Biology

Cited by 20 publications

References 114 publications

Zebrabow: multispectral cell labeling for cell tracing and lineage analysis in zebrafish

Zebrabow: multispectral cell labeling for cell tracing and lineage analysis in zebrafish

Limbal stem cells: Central concepts of corneal epithelial homeostasis

Reconstruction of Functional Ocular Surface by Acellular Porcine Cornea Matrix Scaffold and Limbal Stem Cells Derived from Human Embryonic Stem Cells

Contact Info

Product

Resources

About