Corneal tissue engineering

Huang, Yao‐Xiong

doi:10.1533/9781845699802.1.86

Cited by 3 publications

(2 citation statements)

References 87 publications

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“…OCT images show negligible bioadhesive degradation in PBS over the course of the experiment ( Figure 7 ). Corneal swelling and loss of corneal transparency was observed after 24 h due to ex vivo absorption of large amounts of water by the stromal proteoglycans [ 81 , 82 ]. Importantly, 15 and 20% GelMA bioadhesives remained optically clear throughout the experiment.…”

Section: Discussionmentioning

confidence: 99%

Photocurable GelMA Adhesives for Corneal Perforations

et al. 2022

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The current treatments for the management of corneal and scleral perforations include sutures and adhesives. While sutures are invasive, induce astigmatism and carry a risk of infection, cyanoacrylate glues are toxic, proinflammatory and form an opaque and rough surface that precludes vision. Consequently, the clinical need for a fast curing and strong tissue adhesive with minimised cytotoxicity and host inflammation remains unmet. In this paper, we engineer a gelatine methacryloyl (GelMA) adhesive that can be crosslinked in situ within 2 min using UV or visible light and a riboflavin (RF)/sodium persulfate (SPS) system. Optical coherence tomography (OCT) images demonstrated that the flowable GelMA adhesive could completely fill corneal wounds and restore the ocular curvature by forming a smooth contour on the ocular surface. Further, ex vivo studies in porcine eyes showed that GelMA bioadhesives exhibited burst pressures that were comparable to cyanoacrylates (49 ± 9 kPa), with the hydrogels exhibiting a transmittance (90%), water content (85%) and storage modulus (5 kPa) similar to the human cornea. Finally, using human dermal fibroblasts, we showed that our GelMA adhesive was non-toxic and could effectively support cell adhesion and proliferation. Taken together, the adhesive’s performance, injectability and ease of administration, together with gelatin’s availability and cost-effectiveness, make it a potential stromal filler or sealant for corneal and conjunctival applications.

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Section: Discussionmentioning

confidence: 99%

Photocurable GelMA Adhesives for Corneal Perforations

et al. 2022

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“…Cell sheet is a new technology that has been used in tissue engineering for regeneration and repair of damaged soft tissues. It uses the “thermo‐responsive culture dish” concept which makes the reversible adhesion and detachment of cells from the surface of the dish by changing the surface hydrophobicity of culture vessel . Using this technique, the tissue engineer is capable to produce MSC sheets and then transplant the engineered MSC sheets to the site of injuries such as large bone defects.…”

Section: Mscs Functioning Roles In Bone Tissue Regenerationmentioning

confidence: 99%

Osteogenesis and bone remodeling: A focus on growth factors and bioactive peptides

Toosi

Behravan

2019

BioFactors

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Bone is one of the most frequently transplanted tissues. The bone structure and its physiological function and stem cells biology were known to be closely related to each other for many years. Bone is considered a home to the well‐known systems of postnatal mesenchymal stem cells (MSCs). These bone resident MSCs provide a range of growth factors (GF) and cytokines to support cell growth following injury. These GFs include a group of proteins and peptides produced by different cells which are regulators of important cell functions such as division, migration, and differentiation. GF signaling controls the formation and development of the MSCs condensation and plays a critical role in regulating osteogenesis, chondrogenesis, and bone/mineral homeostasis. Thus, a combination of both MSCs and GFs receives high expectations in regenerative medicine, particularly in bone repair applications. It is known that the delivery of exogenous GFs to the non‐union bone fracture site remarkably improves healing results. Here we present updated information on bone tissue engineering with a specific focus on GF characteristics and their application in cellular functions and tissue healing. Moreover, the interrelation of GFs with the damaged bone microenvironment and their mechanistic functions are discussed.

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Hydrogels as Emerging Materials for Cornea Wound Healing

Khosravimelal

Mobaraki

Eftekhari

et al. 2021

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According to a World Health Organization (WHO) estimation, over 10 million patients suffer from visual impairments and vision loss annually due to corneal diseases or injuries. [6,8] The corneal response to injury involves a highly complicated cascade of events that comprises cell adhesion, migration, differentiation, proliferation, death, and extracellular matrix (ECM) remodeling mediated by immunomodulators and growth factors. [9,10] Conserving the corneal transparency is critical for visual perception. Therefore, any destruction that affects its integrity caused by diseases or trauma like bullous keratopathy, keratoconus, and scarring can lead to blurred eyesight and eventually blindness. [4,11,12] Among all the therapeutic modalities that contributed to corneal irreversible damages including drug and gene therapy and surgical techniques, the keratoplasty with a human donor has offered the best clinical outcome with an 80% rate of success. [2,11,13] However, despite a high success rate, increasing demands for donor tissue and graft rejection due to severe immune response of the hosts are considered as the major reasons for developing an alternative to this procedure. [6,11,13,14] Cell therapies in combination with 3D biocompatible scaffolds have the great potential to replace donor tissue for transplantation or improve wound healing. [15] The potential of cell therapy in corneal wound healing has previously been discussed. [16] 3D scaffolds can mimic the composition and structure of the cornea and

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Corneal tissue engineering

Cited by 3 publications

References 87 publications

Photocurable GelMA Adhesives for Corneal Perforations

Photocurable GelMA Adhesives for Corneal Perforations

Osteogenesis and bone remodeling: A focus on growth factors and bioactive peptides

Hydrogels as Emerging Materials for Cornea Wound Healing

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